Rotavirus and Adenovirus Frequency in Children with Acute Gastroenteritis

Introduction: The most common causes of acute gastroenteritis in children are rotavirus and adenovirus, and laboratory tests are required to make a definitive diagnosis. We aimed to evaluate retrospectively the frequency of rotavirus and adenovirus, detected by immunochromotographic method, in children with acute gastroenteritis referred to our hospital. Materials and Methods: Between January 2013 and September 2014, 3189 children (aged 0-18) with acute gastroenteritis were included into the study. In faecal samples, rotavirus and adenovirus were tested with a qualitative immunochromotographic test (Abon Biopharm Rota/Adeno, China). Patient records were retrospectively analyzed. Chi-square test was used for statistical analysis and the difference was accepted statistically significant when p< 0.05. Results: Viral antigens were determined in 706 (22.1%) of the stool samples. Rotavirus was identified in 17.5%, adenovirus in 3.3%, rotavirus and adenovirus together in 1.3%. There was no statistically significant difference in the frequency of detection of rotavirus and adenovirus among genders (p> 0.05). Rotavirus was most frequently seen between 7-24 months (22%) of ages and between 25 months-4 years (21.2%) of ages (p 0.05). Adenovirus was detected most frequently between 25 months-4 years (4.6%) of ages and lowest among 11 17 years (0.1%) of ages (p= 0.007 and p= 0.0006, respectively). Rotavirus was detected most frequently in spring (25.5%), winter (25.2%), and autumn (23.5%) seasons and the least (5.9%) in summer season (p< 0.005). Conclusion: In conclusion, rotavirus and adenovirus should be routinely investigated in children between 7 months and 4 years, especially in acute gastroenteritis between november and april. Identification of viral agents in children with acute gastroenteritis will help to prevent unnecessary antibiotic administration to provide effective treatment, to prevent heavy dehydration of children and to plan vaccination procedures. Mothers should also be encouraged to breast-feed their babies until they are two years old, as they may have protective effects against viral gastroenteriti

Investigation of the prevalence of visceral leishmaniasis by the microculture method and IFAT in dogs in Kırıkkale

Bu çalışma 2006-2008 yıllarında Kırıkkale'nin farklı yörelerindeki köpeklerde yeni bir yöntem olan Mikrokültür Yöntemi (MKY) ve İndirekt Fluoresan Antikor Testi (IFAT) ile karşılaştırmalı olarak visseral leishmaniosisin prevalansını belirlemek için yapılmıştır. Toplam 50 köpekten alınan kanların tamamı MKY ile negatif olarak saptanmıştır. IFAT ile anti-Leishmania infantum IgG antikorları yönünden incelenmesinde ise sadece 3 yaşındaki Beagle ırkı erkek bir köpekte 1/128 titrede (%2) seropozitiflik tespit edilmiştir.This study was carried out in order to compare the seroprevalence of visceral leishmaniasis in dogs from different areas of Kırıkkale between 2006-2008 using the microculture method (MCM) which is a new method, and the indirect fluorescent antigen test (IFAT). All of the blood collected from total of 50 dogs was found to be negative by MCM. Only one male Beagle strain dog (3 years old) was found to be seropositive at 1/128 titers (2%) for anti-Leishmania infantum IgG antibodies by IFAT