Genetic Transformation, Regeneration and Analysis of Transgenic Peanut

Crop Production/Industries,

Genetic transformation, regeneration and analysis of transgenic peanut

Conventional breeding has led to the improvement of some peanut traits such as seed yield and drought tolerance. However, many important agronomic traits have yet to be improved. The recent development of transformation and regeneration systems has allowed the introduction of useful genes into peanut germplasm. The understanding of disease resistance mechanisms is becoming increasingly advanced so that it should be possible to apply technologies that have been proven in other species to peanut.In this technical report we describe protocols for the routine regeration of transformed peanut plants of spanish and virginia market types based on microprojectile bombardment of embryogenic callus as well as Agrobacterium-mediated transformation of somatic embryos

Comprehensive and Integrated Genomic Characterization of Adult Soft Tissue Sarcomas

Sarcomas are a broad family of mesenchymal malignancies exhibiting remarkable histologic diversity. We describe the multi-platform molecular landscape of 206 adult soft tissue sarcomas representing 6 major types. Along with novel insights into the biology of individual sarcoma types, we report three overarching findings: (1) unlike most epithelial malignancies, these sarcomas (excepting synovial sarcoma) are characterized predominantly by copy-number changes, with low mutational loads and only a few genes (, , ) highly recurrently mutated across sarcoma types; (2) within sarcoma types, genomic and regulomic diversity of driver pathways defines molecular subtypes associated with patient outcome; and (3) the immune microenvironment, inferred from DNA methylation and mRNA profiles, associates with outcome and may inform clinical trials of immune checkpoint inhibitors. Overall, this large-scale analysis reveals previously unappreciated sarcoma-type-specific changes in copy number, methylation, RNA, and protein, providing insights into refining sarcoma therapy and relationships to other cancer types