Developing a measure of blind patients\u27 interactions with their healthcare providers

Background: People who are blind (PWB) are often perceived as being incompetent with common tasks and functions. When patients detect that their healthcare providers (HCPs) have negative perceptions of them, they often report less satisfaction with their healthcare and disengage in their own healthcare. Objective: A scale assessing the experiences of PWB interacting with HCPs was developed and validated across two studies. Methods: In Study 1, 144 participants completed the scale and provided feedback. In Study 2, 214 participants completed the scale and 4 additional scales to assess construct validity. Results: An exploratory factor analysis in Study 1 revealed a two-factor model consisting of General Quality of Health Care (30.5% variance explained) and Stereotype Content-related items (9.4% variance explained). Study 2 confirmed and validated this two-factor structure (RMSEA (90% CI) = 0.068 (0.057, 0.079), CFI = 0.898, SRMR = 0.066, AIC = 14568.902). Conclusions: This scale is one of the first tools developed from the perspectives of PWB. Results from these studies highlight and elaborate on how PWB perceive that they are viewed by their HCPs in terms of competence and how they perceive to be treated by these HCPs. This scale can be used in training HCPs to better serve their patients with disabilities

Study of effect of arbuscular mycorrhiza (Glomus intraradices) fungus on wheat under nickel stress

ArticleIn many regions of the world soils are contaminated with heavy metals and therefore restricted in their use. For instance, the absorption of nickel (Ni) in the tissue of plants increase the plant’s metabolism and cause physiological disorders or even death. Arbuscular mycorrhizal fungi are known to enhance the tolerance of host plants to abiotic and biotic stress. Thus, we investigated the potential of the arbuscular m ycorrhizal fungi Glomus intraradices to mitigate deleterious effects of Ni in wheat. The experiment was conducted using four levels of Ni (0, 60, 120 and 180 mg per kg of soil) and two levels of mycorrhizal fungi application (with and without Glomus intrar adices ). Nickel stress significantly decreased seed number per spike, thousand - seed weight, seed yield per plant, concentration of chlorophyll a and b. At the same time, we found increased catalase (CAT) enzyme activity and dityrosine (DT) treatments. Mycorrhizal fungi application attenuated Ni effects, i.e. fungal presence increased seed number per spike, thousand - seed weight, chlorophyll a and b. Furthermore mycorrhizal fungi application reduce CAT enzyme activity and DT. In general, our results sugge st that mycorrhizal fungi application reduces harmful effects of Ni stress in wheat

Effect of gallic acid on Alkaline phosphatase gene expression in vascular smooth muscle cells

Background and purpose: Vascular calcification is an important factor in pathogenesis of atherosclerosis. Studies have shown that alkaline phosphatase increases vascular calcification. Here we investigated the effect of gallic acid on alkaline phosphatase gene expression in vascular smooth muscle cells (VSMCs). Materials and methods: In this experimental study humans aorta VSMCs were incubated with beta glycerol phosphate as calcification-inducing media. Then these cells were treated with 160, 180 and 200 µMol concentration of gallic acid for 24h, 48h and 72h. The total RNA was extracted and cDNA was synthesized and then alkaline phosphatase expression was measured by real time PCR. Alkaline phosphatase specific activity was measured by spectrophotometry. Results: Overall, 160, 180 and 200 µMol concentration of gallic acid decreased alkaline phosphatase gene expression in vascular smooth muscle cell by 1.98, 2.03, and 3.16 folds, respectively after 72h compared with the control group. The alkaline phosphatase specific activity also decreased compared to that of the control group. Conclusion: Our results showed that gallic acid decreased the expression and activity of alkaline phosphatase suggesting that this antioxidant compound may attenuate vascular calcification

The underrepresentation of Latinx students in the professional dissemination of psychology research

Latinx students are well represented among undergraduate psychology majors. However, there is an underrepresentation of Latinxs in psychology graduate programs, among faculty, and licensed practitioners. This underrepresentation is evidence of a leaky pipeline of attrition among Latinx psychology students from bachelor to postbachelor career tracks. The present research investigates one point of this leaky pipeline—research activity and professional dissemination of research. We used public data sets and surname matching to compare Latinx student representation at a regional psychology conference to Latinx enrollment and psychology degree completions at colleges and universities represented at the conference. We found consistent evidence of Latinx underrepresentation in the professional dissemination of research. This underrepresentation occurred at both federally designated Hispanic-serving institutions (HSIs) and non-HSIs. This research shows one point in the academic pipeline in psychological science at which Latinx students are underrepresented. Results provide evidence for a lack of recruitment of Latinx students into the research process. This disparity in research activity may lead to attrition from undergraduate to postbachelor education and career tracks in psychology. Strategies that faculty and departments can employ to address these disparities are discussed

Development and Validation of a Measure for Seeking Health Information in the Diabetes Online Community: Mixed Methods Study

Background Individuals with chronic diseases often search for health information online. The Diabetes Online Community (DOC) is an active community with members who exchange health information; however, few studies have examined health information brokering in the DOC. Objective The aim of this study was to develop and validate the Attitudes Toward Seeking Health Information Online (ATSHIO) scale in a sample of adults with type 1 diabetes (T1D). Methods People with T1D were recruited through the DOC, specifically Facebook and Twitter. They were provided with a Qualtrics link to complete the survey. This was a mixed methods study that used thematic analysis along with existing theory and formative research to design the quantitative ATSHIO scale. Results A total of 166 people with T1D participated in this study. Confirmatory factor analyses determined a 2-factor scale (Trusting and Evaluating Online Health Information in the DOC and Engaging With Online Health Information in the DOC) with good convergent validity and discriminant validity. Correlations were found between social support, online health information–seeking, diabetes distress, and disease management. Conclusions The ATSHIO scale can be used to investigate how people with diabetes are using the internet for obtaining health information, which is especially relevant in the age of telehealth and Health 2.0

“It’s embarrassing. I get angry. I get frustrated.”: Understanding severe hypoglycemia and glucagon usage from the perspectives of people with type 1 diabetes

Introduction This study characterized the emotional impact of severe hypoglycemia, views of glucagon, and barriers to glucagon use from the perspective of adults with type 1 diabetes (T1D). Methods Participants included individuals recruited from the T1D Exchange online community. The current study conducted 7 focus groups consisting of adults with T1D (N = 38, average age 49.4, SD = 16.11 years). Average duration of diabetes was 34.4 years (SD = 17.3) and average self-reported A1c was 6.8 % (SD = 0.7). Focus group interviews were recorded, transcribed, and thematically analyzed. Results A range of emotions was expressed about severe hypoglycemia including fear, anxiety, stress, frustration, shame, and embarrassment. Participants frequently identified prescription cost and insurance deductibles as barriers to glucagon use. Participants were also concerned about ease of administration—how difficult it is to prepare the glucagon in an emergency. Many participants expressed a preference for auto-injectables over nasal administration. Timing of glucagon action and time to recovery were high priorities. Some participants, while they had not self-administered glucagon, were interested in a mini-dose glucagon they could self-administer. They also identified desirable characteristics of glucagon treatment including reduced cost, long shelf-life, and quick activation. Conclusions These results highlight the attitudes about severe hypoglycemia and emergency treatment with glucagon. Healthcare professionals should assess glucagon training needs and knowledge when they meet with their patients with diabetes

Smoking Social Norms Among Spanish-Speaking Mexican-Origin Persons Who Smoke

In the United States, smoking rates increase with greater acculturation among Mexican-origin women, but not among men. Conversely, greater acculturation is associated with higher likelihood of quitting among Mexican-origin men who smoke, but not among women who smoke. Long-standing speculation is that adoption of smoking social norms in the U.S. that are less restrictive for women and more restrictive for men compared to smoking social norms in Mexico may account for these patterns. However, it is unknown whether persons who smoke actually perceive such differential norms. The current study characterized smoking social norms in the U.S. and Mexico among Spanish-speaking Mexican-origin persons who smoke. Two hundred and ninety Mexican-origin persons who smoke were surveyed on descriptive and injunctive norms for men and women in the U.S. and Mexico. Estimated means for smoking social norms in the U.S. and Mexico were compared separately among men and women. Among men, mean descriptive and injunctive norms in Mexico were significantly higher than those for the U.S. Among women, neither mean descriptive nor injunctive norms were significantly different between the U.S. and Mexico. Mexican-origin women who smoke perceive smoking among women as equally common and similarly unacceptable in the U.S. and Mexico. Findings do not support speculation that differential social norms may explain the acculturation-smoking relationship among Mexican-origin women. Mexican-origin men who smoke perceive smoking among men both less common and less acceptable in the U.S. compared to Mexico. Social norms should be investigated as a mechanism of the acculturation-cessation relationship among Mexican-origin men. Understanding direct and indirect influences of social norms on cessation among Mexican-origin men stands to inform tailoring of interventions

Tear Deformation Time and optical quality in eyes wearing silicone hydrogel contact lenses

Purpose To evaluate tear film stability and optical aberrations in eyes wearing plano Lotrafilcon B contact lenses (CL). Methods Tear Deformation Time (TDT) and aberrometric parameters were assessed in 86 normal emmetropic eyes before and 6 h after wearing CL. Result A statistically significant decrease in TDT and increase in root mean square (RMS) values of higher order aberrations (HOA) were revealed 6 h after CL insertion (both P < 0.001). The low order aberrations (LOA) RMS values measured with CL were higher than those in naked eyes, but the difference was not statistically significant. None of individual Zernike polynomials showed any significant alteration. Conclusion Our findings indicated that the Lotrafilcon B contact lens affects HOA more than LOA. The tear film was less stable after wearing CL. © 2016 Iranian Society of Ophthalmolog

Associations between parenting strategies and BMI percentile among Latino children and youth with intellectual and developmental disabilities

Introduction: Maintaining healthy weight is a challenge for all children, and particularly for children with IDD compared to nondisabled children and for Latino children compared to non-Latino White children. Parenting practices related to food intake and physical activity have been found to be important in maintaining children\u27s weight. In this study, we describe the prevalence of overweight and obesity status among Latino children with IDD and their maternal caregivers and determine the relationship between food and physical activity parenting practices and childhood obesity among Latino children with IDD. Methods: We interviewed 94 Latino parent/child dyads and collected information about parenting practices, home environment, and parent and child height and weight using standardized measures. Parent body mass index (BMI) and child BMI percentile were calculated from height and weight. Results: The combined overweight/obesity status for children in our sample was high (60.3%) compared to national rates among nondisabled Latino children (56%) and non-Latino White children with autism (37%). Contrary to research on nondisabled children, we found that greater parental use of controlling dietary strategies was associated with lower BMI percentile in Latino children with IDD. These findings may be indicative of the fact that children with IDD tend to have unique dietary behaviors that warrant more disability and culturally sensitive strategies. Discussion: Our findings suggest that overweight and obesity is especially prevalent for Latino children with IDD and that more research is needed on family factors that promote health in Latino families of children with IDD

HSP70/IL-2 treated NK cells effectively cross the blood brain barrier and target tumor cells in a rat model of induced glioblastoma multiforme (GBM)

Natural killer (NK) cell therapy is one of the most promising treatments for Glioblastoma Multiforme (GBM). However, this emerging technology is limited by the availability of sufficient numbers of fully functional cells. Here, we investigated the efficacy of NK cells that were expanded and treated by interleukin-2 (IL-2) and heat shock protein 70 (HSP70), both in vitro and in vivo. Proliferation and cytotoxicity assays were used to assess the functionality of NK cells in vitro, after which treated and naïve NK cells were administrated intracranially and systemically to compare the potential antitumor activities in our in vivo rat GBM models. In vitro assays provided strong evidence of NK cell efficacy against C6 tumor cells. In vivo tracking of NK cells showed efficient homing around and within the tumor site. Furthermore, significant amelioration of the tumor in rats treated with HSP70/Il-2-treated NK cells as compared to those subjected to nontreated NK cells, as confirmed by MRI, proved the efficacy of adoptive NK cell therapy. Moreover, results obtained with systemic injection confirmed migration of activated NK cells over the blood brain barrier and subsequent targeting of GBM tumor cells. Our data suggest that administration of HSP70/Il-2-treated NK cells may be a promising therapeutic approach to be considered in the treatment of GBM. © 2020 by the authors. Licensee MDPI, Basel, Switzerland