Effects of a Dispersed and Undispersed Crude Oil on Mangroves, Seagrasses and Corals

The primary objective of this study was to evaluate the application of dispersant to spilled oil as a means of reducing adverse environmental effects of oil spills in nearshore, tropical waters. The results of numerous laboratory and field studies have suggested that dispersants may play a useful role in reducing adverse impacts on sensitive and valued environments such as mangroves, seagrasses, and corals. However, the use of dispersants has not been allowed thus far in most situations because of a lack of direct experimental data on the various effects of dispersants and the environmental trade-offs presumed to occur as a result of their application to crude oils. To accomplish this objective, a 21/2- year field experiment was designed in which detailed, synoptic measurements and assessments were made of representative intertidal and nearshore subtidal habitats and organisms (man-groves, seagrass beds, and coral reefs) before, during, and after exposure to untreated crude oil and chemically dispersed oil. The results were in-tended to give guidance in minimizing the ecological impacts of oil spills through evaluation of trade-offs in the relative impacts of chemical dispersion to tropical marine intertidal and subtidal habitats

Spectroscopic and Microscopic Characterization of Contaminant Uptake and Retention by Carbonates in the Soil and Vadose Zone

The research focus of this previous EMSP grant was assessment of the role that carbonate minerals play in the uptake and sequestration of metal and radionuclide contaminants in soils and the vadose zone for conditions relevant to the Hanford Site and other sites in the DOE Complex. The project was a collaboration among researchers at SUNY-Stony Brook and EMSL/PNNL. Carbonates, particularly calcite, are present in the Hanford subsurface as grain coatings, disseminated particles, and dense caliche layers. Calcite is also predicted to be forming beneath leaking tanks. A range of metal and radionuclide species that pose risks at Hanford and othe DOE istes were considered, including u(VI), Cr(CV), Cs, Pb(II), and selected lanthanides (as models for trivalent actinides). Batch sorption and co-precipitation experiments of these metals with pre-equilibrated calcite and selected uptake experiments on natural caliche formed the basis to determine the mechanisms of metal/radionuclide binding and to assess the effect on the stability of the sorbed species and the potential for remobilization. Our results provide ne information that can benefit DOE clean-up methodology and potentially provide new approaches for uptake of selected heavy metal

A budding yeast model for human disease mutations in the EXOSC2 cap subunit of the RNA exosome complex

RNA exosomopathies, a growing family of diseases, are linked to missense mutations in genes encoding structural subunits of the evolutionarily conserved, 10-subunit exoribonuclease complex, the RNA exosome. This complex consists of a three-subunit cap, a six-subunit, barrel-shaped core, and a catalytic base subunit. While a number of mutations in RNA exosome genes cause pontocerebellar hypoplasia, mutations in the cap subunit gene EXOSC2 cause an apparently distinct clinical presentation that has been defined as a novel syndrome SHRF (short stature, hearing loss, retinitis pigmentosa, and distinctive facies). We generated the first in vivo model of the SHRF pathogenic amino acid substitutions using budding yeast by modeling pathogenic EXOSC2 missense mutations (p.Gly30Val and p.Gly198Asp) in the orthologous S. cerevisiae gene RRP4 The resulting rrp4 mutant cells show defects in cell growth and RNA exosome function. Consistent with altered RNA exosome function, we detect significant transcriptomic changes in both coding and noncoding RNAs in rrp4-G226D cells that model EXOSC2 p.Gly198Asp, suggesting defects in nuclear surveillance. Biochemical and genetic analyses suggest that the Rrp4 G226D variant subunit shows impaired interactions with key RNA exosome cofactors that modulate the function of the complex. These results provide the first in vivo evidence that pathogenic missense mutations present in EXOSC2 impair the function of the RNA exosome. This study also sets the stage to compare exosomopathy models to understand how defects in RNA exosome function underlie distinct pathologies

Suction cup sampler bias in leaching characterization of an undisturbed field soil

An accurate assessment of leaching losses in the vadose zone requires measurement of both solute and water flux to compute flux concentrations (C[subscript F]). Leachate collected at a depth of 1.2 m in 32 passive capillary samplers (PCAPS), which sample soil-pore water continuously at tensions of 0–50 cm H₂O was compared to that collected in 32 suction cup samplers operated under a falling head vacuum of 530-cm H₂O over a 2-year period. There was evidence that PCAPS collected CF and suction cup samplers collected resident concentrations (C[subscript R]) as shown by the earlier breakthrough of a bromide tracer in the PCAPS as compared to the suction cup samplers. C[subscript R] was up to 100% lower than C[subscript F] during the rising branch of Br tracer breakthrough and up to 78% greater during the declining branch of breakthrough. Br content and water flux into PCAPS were correlated with correlation coefficients changing from positive to negative values with the advancement of the tracer breakthrough peak through the profile indicating the declining importance of preferential flow on Br transport. C[subscript R] and C[subscript F] differed significantly (P < 0.05) for 35% of the sampling events for NO₃, but seasonal means were mostly insignificantly different for this regularly applied and therefore more uniformly distributed anion. The early breakthrough of Rhodamine WT and Brilliant Blue FCF, which was applied with the Br, was very low with 0.15% and 0.08% of the initial concentration C₀, respectively, with C[subscript R] differing from C[subscript F] by up to −100%. For all tracers, mass leached using C[subscript R] is therefore prone to bias for short-term (<0.6 pore volumes) monitoring.Keywords: Hydrology: Unsaturated zone, Hydrology: Groundwater quality, Hydrology: Groundwater hydrology, Hydrology: Instruments and technique

The Changing Nature of Poverty

Since the beginning of the War on Poverty, the poverty rate has fluctuated widely, and at the same time the poverty population has undergone many changes, some mirroring the changing stereotypes of the poor and others less pronounced than the changing stereotypes would lead us to believe. A feminization of poverty has occurred, with many more of the poor now in households headed by women. Interestingly, aging of the poverty population has not occurred despite growth in the elderly segment of the overall population. Concerning turnover in the poverty population, we find that despite poverty theories emphasizing persistence, recurrent poverty is relatively rare and poverty is not generally passed from one generation to the next. Poverty prevention has come from both economic growth and government transfers; however, inequality in economic growth has contributed to poverty. With the proportion of elderly and female- headed households likely to continue at a high level into the future, poverty rates are also likely to remain high unless government transfers are increased.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66979/2/10.1177_000271628547900103.pd

Pseudogap-less high Tc_{c} superconductivity in BaCox_{x}Fe2−x_{2-x}As2_{2}

The pseudogap state is one of the peculiarities of the cuprate high temperature superconductors. Here we investigate its presence in BaCo$_{x}$Fe$_{2-x}$As$_{2}$, a member of the pnictide family, with temperature dependent scanning tunneling spectroscopy. We observe that for under, optimally and overdoped systems the gap in the tunneling spectra always closes at the bulk T$_{c}$, ruling out the presence of a pseudogap state. For the underdoped case we observe superconducting gaps over large fields of view, setting a lower limit of tens of nanometers on the length scale of possible phase separated regions.Comment: 5 pages, 3 figure

Correction:Variability and cost implications of three generations of the Roche LightCycler® 480

[This corrects the article DOI: 10.1371/journal.pone.0190847.]

Potential Deaths Averted and Serious Adverse Events Incurred From Adoption of the SPRINT (Systolic Blood Pressure Intervention Trial) Intensive Blood Pressure Regimen in the United States: Projections From NHANES (National Health and Nutrition Examination Survey)

BACKGROUND: SPRINT (Systolic Blood Pressure Intervention Trial) demonstrated a 27% reduction in all-cause mortality with a systolic blood pressure (SBP) goal ofmellitus, stroke, or heart failure. To quantify the potential benefits and risks of SPRINT intensive goal implementation, we estimated the deaths prevented and excess serious adverse events incurred if the SPRINT intensive SBP treatment goal were implemented in all eligible US adults. METHODS: SPRINT eligibility criteria were applied to the 1999 to 2006 National Health and Nutrition Examination Survey and linked with the National Death Index through December 2011. SPRINT eligibility included age ≥50 years, SBP of 130 to 180 mm Hg (depending on the number of antihypertensive medications being taken), and high cardiovascular disease risk. Exclusion criteria were diabetes mellitus, history of stroke, \u3e1 g proteinuria, heart failure, estimated glomerular filtration ratemL·min RESULTS: The mean age was 68.6 years, and 83.2% and 7.4% were non-Hispanic white and non-Hispanic black, respectively. The annual mortality rate was 2.20% (95% confidence interval [CI], 1.91-2.48), and intensive SBP treatment was projected to prevent ≈107 500 deaths per year (95% CI, 93 300-121 200) and give rise to 56 100 (95% CI, 50 800-61 400) episodes of hypotension, 34 400 (95% CI, 31 200-37 600) episodes of syncope, 43 400 (95% CI, 39 400-47 500) serious electrolyte disorders, and 88 700 (95% CI, 80 400-97 000) cases of acute kidney injury per year. The analysis-of-extremes approach indicated that the range of estimated lower- and upper-bound number of deaths prevented per year with intensive SBP control was 34 600 to 179 600. Intensive SBP control was projected to prevent 46 100 (95% CI, 41 800-50 400) cases of heart failure annually. CONCLUSIONS: If fully implemented in eligible US adults, intensive SBP treatment could prevent ≈107 500 deaths per year. A consequence of this treatment strategy, however, could be an increase in serious adverse events