A Type III Polyketide Synthase Specific for Sporulating Negativicutes is Responsible for Alkylpyrone Biosynthesis

Genomic analyses indicate that anaerobic bacteria represent a neglected source of natural products. Whereas a limited number of polyketides have been reported from anaerobes, products of type III polyketide synthases (PKSs) have remained unknown. We found a highly conserved biosynthetic gene cluster (BGC) comprising genes putatively encoding a type III PKS and a methyltransferase in genomes of the Negativicutes, strictly anaerobic, diderm bacteria. By in vivo and in vitro expression of a type III PKS gene, dquA from the oak‐associated Dendrosporobacter quercicolus in E. coli we show production of long‐chain alkylpyrones. Intriguingly, this BGC is specific for sporulating Sporomusaceae but absent in related Negativicutes that do not sporulate, thus suggesting a physiological role

Recyclable Architecture: Prefabricated and Recyclable Typologies

Buildings are being demolished without taking into the account the waste generated, and the housing shortage problem is getting more critical as cities are growing and the demand for built space and the use of resources are increasing. Architectural projects have been using prefabrication and modular systems to solve these problems. However, there is an absence of structures that can be disassembled and reused when the structure’s life ran its course. This paper presents three building prototypes of new recyclable architectural typologies: (i) a Slab prototype designed as a shelf structure where wooden housing modules can be plugged in and out, (ii) a Tower prototype allowing for an easy change of layout and use of different floors and (iii) a Demountable prototype characterized by the entire demountability of the building. These typologies combine modularity, flexibility, and disassembling to address the increasing demands for multi-use, re-usable and resource-efficient constructions. Design, drawings, plans, and 3D models are developed, tested and analyzed as a part of the research. The results show that the implementation of the recyclable architectural concept at the first design stage is feasible and realistic, and ensures the adaptation through time, increases life span, usability and the material reusability, while avoiding demolition, which in turn reduces the construction waste and, consequently, the CO2 emissions

Recyclable Architecture: Prefabricated and Recyclable Typologies

Buildings are being demolished without taking into the account the waste generated, and the housing shortage problem is getting more critical as cities are growing and the demand for built space and the use of resources are increasing. Architectural projects have been using prefabrication and modular systems to solve these problems. However, there is an absence of structures that can be disassembled and reused when the structure’s life ran its course. This paper presents three building prototypes of new recyclable architectural typologies: (i) a Slab prototype designed as a shelf structure where wooden housing modules can be plugged in and out, (ii) a Tower prototype allowing for an easy change of layout and use of different floors and (iii) a Demountable prototype characterized by the entire demountability of the building. These typologies combine modularity, flexibility, and disassembling to address the increasing demands for multi‐use, re‐usable and resource‐efficient constructions. Design, drawings, plans, and 3D models are developed, tested and analyzed as a part of the research. The results show that the implementation of the recyclable architectural concept at the first design stage is feasible and realistic, and ensures the adaptation through time, increases life span, usability and the material reusability, while avoiding demolition, which in turn reduces the construction waste and, consequently, the CO2 emissions

Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals

Secondary metabolites have a great potential as pharmaceuticals, but there are only a few examples where regulation of gene cluster expression has been correlated with ecological and physiological relevance for the producer. Here, signals, mediators, and biological effects of terrein production were studied in the fungus Aspergillus terreus to elucidate the contribution of terrein to ecological competition. Terrein causes fruit surface lesions and inhibits plant seed germination. Additionally, terrein is moderately antifungal and reduces ferric iron, thereby supporting growth of A. terreus under iron starvation. In accordance, the lack of nitrogen or iron or elevated methionine levels induced terrein production and was dependent on either the nitrogen response regulators AreA and AtfA or the iron response regulator HapX. Independent signal transduction allows complex sensing of the environment and, combined with its broad spectrum of biological activities, terrein provides a prominent example of adapted secondary metabolite production in response to environmental competition