Nanostructured Lipid Carriers Made of Ω-3 Polyunsaturated Fatty Acids: In Vitro Evaluation of Emerging Nanocarriers to Treat Neurodegenerative Diseases

Neurodegenerative diseases (ND) are one of the main problems of public health systems in the 21st century. The rise of nanotechnology-based drug delivery systems (DDS) has become in an emerging approach to target and treat these disorders related to the central nervous system (CNS). Among others, the use of nanostructured lipid carriers (NLCs) has increased in the last few years. Up to today, most of the developed NLCs have been made of a mixture of solid and liquid lipids without any active role in preventing or treating diseases. In this study, we successfully developed NLCs made of a functional lipid, such as the hydroxylated derivate of docohexaenoic acid (DHAH), named DHAH-NLCs. The newly developed nanocarriers were around 100 nm in size, with a polydispersity index (PDI) value of <0.3, and they exhibited positive zeta potential due to the successful chitosan (CS) and TAT coating. DHAH-NLCs were shown to be safe in both dopaminergic and microglia primary cell cultures. Moreover, they exhibited neuroprotective effects in dopaminergic neuron cell cultures after exposition to 6-hydroxydopamine hydrochloride (6-OHDA) neurotoxin and decreased the proinflammatory cytokine levels in microglia primary cell cultures after lipopolysaccharide (LPS) stimuli. The levels of the three tested cytokines, IL-6, IL-1β and TNF-α were decreased almost to control levels after the treatment with DHAH-NLCs. Taken together, these data suggest the suitability of DHAH-NLCs to attaining enhanced and synergistic effects for the treatment of NDs.This project was partially supported by the Spanish Ministry of Economy and Competitiveness (RTC-2015-3542-1) and the Basque Government (Consolidated Groups, IT 907-16)

Increased antiparkinson efficacy of the combined administration of VEGF- and GDNF-loaded nanospheres in a partial lesion model of Parkinson's disease

Current research efforts are focused on the application of growth factors, such as glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF), as neuroregenerative approaches that will prevent the neurodegenerative process in Parkinson's disease. Continuing a previous work published by our research group, and with the aim to overcome different limitations related to growth factor administration, VEGF and GDNF were encapsulated in poly(lactic-co-glycolic acid) nanospheres (NS). This strategy facilitates the combined administration of the VEGF and GDNF into the brain of 6-hydroxydopamine (6-OHDA) partially lesioned rats, resulting in a continuous and simultaneous drug release. The NS particle size was about 200 nm and the simultaneous addition of VEGF NS and GDNF NS resulted in significant protection of the PC-12 cell line against 6-OHDA in vitro. Once the poly(lactic-co-glycolic acid) NS were implanted into the striatum of 6-OHDA partially lesioned rats, the amphetamine rotation behavior test was carried out over 10 weeks, in order to check for in vivo efficacy. The results showed that VEGF NS and GDNF NS significantly decreased the number of amphetamine-induced rotations at the end of the study. In addition, tyrosine hydroxylase immunohistochemical analysis in the striatum and the external substantia nigra confirmed a significant enhancement of neurons in the VEGF NS and GDNF NS treatment group. The synergistic effect of VEGF NS and GDNF NS allows for a reduction of the dose by half, and may be a valuable neurogenerative/neuroreparative approach for treating Parkinson's disease.The "Ministerio de Ciencia e Innovacion" (SAF2010-20375), the University of the Basque Country (UPV/EHU) (UFI 11/32), the Basque Government (Saiotek SA-2010/00028), and FEDER funds partially supported this project. The authors are grateful for the cooperation of SGIker (UPV/EHU, MICINN, GV/EJ, ESF). E Herran appreciates the Basque Government fellowship subvention, and C Requejo and A Aristieta thank UPV/EHU for a fellowship subvention. We thank Dr A Krzyzanowska for the careful revision of the manuscript

Nanopartícula lipídica de tobramicina

[EN] The invention relates to: a lipid nanoparticle comprising at least one tobramycin antibiotic, a lipid fraction, and one or more surfactants; and the use of the nanoparticle in the prevention and/or treatment of infections of the respiratory tree.[ES] La presente invención se relaciona con una nanopartícula lipídica que comprende al menos un antibiótico tobramicina, una fracción lipídica y uno o más tensioactivos, y el uso de la nanopartícula en la prevención y/o tratamiento de infecciones del árbol respiratorio.Biopraxis Research AIE, Universidad del Pais Vasco, Universidad de Barcelona, Fundació D'investigació Sanitària De Les Illes Balears, Consejo Superior de Investigaciones Científicas (España)A1 Solicitud de patente con informe sobre el estado de la técnic

Lipid nanoparticle of tobramycin

[ES] La presente invención se relaciona con una nanopartícula lipídica que comprende al menos un antibiótico tobramicina, una fracción lipídica y uno o más tensioactivos, y el uso de la nanopartícula en la prevención y/o tratamiento de infecciones del árbol respiratorio.[EN] The invention relates to: a lipid nanoparticle comprising at least one tobramycin antibiotic, a lipid fraction, and one or more surfactants; and the use of the nanoparticle in the prevention and/or treatment of infections of the respiratory tree.Peer reviewedBiopraxis Research AIE, Universidad del Pais Vasco, Universidad de Barcelona, Fundació D'investigació Sanitària De Les Illes Balears, Consejo Superior de Investigaciones Científicas (España)A1 Solicitud de patente con informe sobre el estado de la técnic