12,915 research outputs found

    Copyright Protection of Color Imaging Using Robust-Encoded Watermarking

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    In this paper we present a robust-encoded watermarking method applied to color images for copyright protection, which presents robustness against several geometric and signal processing distortions. Trade-off between payload, robustness and imperceptibility is a very important aspect which has to be considered when a watermark algorithm is designed. In our proposed scheme, previously to be embedded into the image, the watermark signal is encoded using a convolutional encoder, which can perform forward error correction achieving better robustness performance. Then, the embedding process is carried out through the discrete cosine transform domain (DCT) of an image using the image normalization technique to accomplish robustness against geometric and signal processing distortions. The embedded watermark coded bits are extracted and decoded using the Viterbi algorithm. In order to determine the presence or absence of the watermark into the image we compute the bit error rate (BER) between the recovered and the original watermark data sequence. The quality of the watermarked image is measured using the well-known indices: Peak Signal to Noise Ratio (PSNR), Visual Information Fidelity (VIF) and Structural Similarity Index (SSIM). The color difference between the watermarked and original images is obtained by using the Normalized Color Difference (NCD) measure. The experimental results show that the proposed method provides good performance in terms of imperceptibility and robustness. The comparison among the proposed and previously reported methods based on different techniques is also provided

    Robust Object-Based Watermarking Using SURF Feature Matching and DFT Domain

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    In this paper we propose a robust object-based watermarking method, in which the watermark is embedded into the middle frequencies band of the Discrete Fourier Transform (DFT) magnitude of the selected object region, altogether with the Speeded Up Robust Feature (SURF) algorithm to allow the correct watermark detection, even if the watermarked image has been distorted. To recognize the selected object region after geometric distortions, during the embedding process the SURF features are estimated and stored in advance to be used during the detection process. In the detection stage, the SURF features of the distorted image are estimated and match them with the stored ones. From the matching result, SURF features are used to compute the Affine-transformation parameters and the object region is recovered. The quality of the watermarked image is measured using the Peak Signal to Noise Ratio (PSNR), Structural Similarity Index (SSIM) and the Visual Information Fidelity (VIF). The experimental results show the proposed method provides robustness against several geometric distortions, signal processing operations and combined distortions. The receiver operating characteristics (ROC) curves also show the desirable detection performance of the proposed method. The comparison with a previously reported methods based on different techniques is also provided

    Improving a Satellite Mission System by means of a Semantic Grid Architecture

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    The use of a semantic grid architecture can make easier the deployment of complex applications, in which several organizations are involved and diverse resources are shared. This paper presents the application of the architecture defined in the Ontogrid project (S-OGSA) into a scenario for the analysis of the quality of the products of satellite missions

    Two modes of Gulf Stream variability revealed in the last two decades of satellite altimeter data

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    Author Posting. © American Meteorological Society, 2014. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 44 (2014): 149–163, doi:10.1175/JPO-D-13-0136.1.Monthly mapped sea level anomalies (MSLAs) of the NW Atlantic in the region immediately downstream of the Gulf Stream (GS) separation point reveal a leading mode in which the path shifts approximately 100 km meridionally about a nominal latitude of 39°N, producing coherent sea level anomaly (SLA) variability from 72° to 50°W. This mode can be captured by use of a simple 16-point index based on SLA data taken along the maximum of the observed variability in the region 33°–46°N and 45°–75°W. The GS shifts between 2010 and 2012 are the largest of the last decade and equal to the largest of the entire record. The second group of EOF modes of variability describes GS meanders, which propagate mainly westward interrupted by brief periods of eastward or stationary meanders. These meanders have wavelengths of approximately 400 km and can be seen in standard EOFs by spatial phase shifting of a standing meander pattern in the SLA data. The spectral properties of these modes indicate strong variability at interannual and longer periods for the first mode and periods of a few to several months for the meanders. While the former is quite similar to a previous use of the altimeter for GS path, the simple index is a useful measure of the large-scale shifts in the GS path that is quickly estimated and updated without changes in previous estimates. The time-scale separation allows a low-pass filtered 16-point index to be reflective of large-scale, coherent shifts in the GS path.Agencia Canaria de Investigación, Innovación y Sociedad de la Información (ACIISI) grant program of Apoyo al Personal Investigador en Formación and NSF Grant OCE-07267202014-07-0

    New Insights into the Nucleolar Localization of a Plant RNA Virus-Encoded Protein That Acts in Both RNA Packaging and RNA Silencing Suppression: Involvement of Importins Alpha and Relevance for Viral Infection

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    [EN] Despite the fact that replication of plus-strand RNA viruses takes place in the cytoplasm of host cells, different proteins encoded by these infectious agents have been shown to localize in the nucleus, with high accumulation at the nucleolus. In most cases, the molecular determinants or biological significance of such subcellular localization remains elusive. Recently, we reported that protein p37 encoded by Pelargonium line pattern virus (family Tombusviridae) acts in both RNA packaging and RNA silencing suppression. Consistently with these functions, p37 was detected in the cytoplasm of plant cells, although it was also present in the nucleus and, particularly, in the nucleolus. Here, we searched for further insights into factors influencing p37 nucleolar localization and into its potential relevance for viral infection. Besides mapping the protein region containing the nucleolar localization signal, we have found that p37 interacts with distinct members of the importin alpha familymain cellular transporters for nucleo-cytoplasmic traffic of proteins-and that these interactions are crucial for nucleolar targeting of p37. Impairment of p37 nucleolar localization through downregulation of importin alpha expression resulted in a reduction of viral accumulation, suggesting that sorting of the protein to the major subnuclear compartment is advantageous for the infection process.This work was supported by Ministerio de Economia y Competitividad (MINECO, Spain) Fundo Europeu de Desenvolvimento Regional (FEDER) (grant BFU2015-70261 to C. Hernandez). M. Perez-Canamas was the recipient of a predoctoral contract from MINECO-FEDER.Perez-Cañamas, M.; Hernandez Fort, C. (2018). New Insights into the Nucleolar Localization of a Plant RNA Virus-Encoded Protein That Acts in Both RNA Packaging and RNA Silencing Suppression: Involvement of Importins Alpha and Relevance for Viral Infection. Molecular Plant-Microbe Interactions. 31(11):1134-1144. https://doi.org/10.1094/MPMI-02-18-0050-RS11341144311

    Bacterial Active Community Cycling in Response to Solar Radiation and Their Influence on Nutrient Changes in a High-Altitude Wetland

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    IndexaciĂłn: Web of Science; Scopus.Microbial communities inhabiting high-altitude spring ecosystems are subjected to extreme changes in solar irradiance and temperature throughout the diel cycle. Here, using 16S rRNA gene tag pyrosequencing (cDNA) we determined the composition of actively transcribing bacteria from spring waters experimentally exposed through the day (morning, noon, and afternoon) to variable levels of solar radiation and light quality, and evaluated their influence on nutrient recycling. Solar irradiance, temperature, and changes in nutrient dynamics were associated with changes in the active bacterial community structure, predominantly by Cyanobacteria, Verrucomicrobia, Proteobacteria, and 35 other Phyla, including the recently described Candidate Phyla Radiation (e.g., Parcubacteria, Gracilibacteria, OP3, TM6, SR1). Diversity increased at noon, when the highest irradiances were measured (3.3-3.9 H', 1125 W m(-2)) compared to morning and afternoon (0.6-2.8 H'). This shift was associated with a decrease in the contribution to pyrolibraries by Cyanobacteria and an increase of Proteobacteria and other initially low frequently and rare bacteria phyla (< 0.5%) in the pyrolibraries. A potential increase in the activity of Cyanobacteria and other phototrophic groups, e.g., Rhodobacterales, was observed and associated with UVR, suggesting the presence of photo activated repair mechanisms to resist high levels of solar radiation. In addition, the percentage contribution of cyanobacterial sequences in the afternoon was similar to those recorded in the morning. The shifts in the contribution by Cyanobacteria also influenced the rate of change in nitrate, nitrite, and phosphate, highlighted by a high level of nitrate accumulation during hours of high radiation and temperature associated with nitrifying bacteria activity. We did not detect ammonia or nitrite oxidizing bacteria in situ, but both functional groups (Nitrosomona and Nitrospira) appeared mainly in pyrolibraries generated from dark incubations. In total, our results reveal that both the structure and the diversity of the active bacteria community was extremely dynamic through the day, and showed marked shifts in composition that influenced nutrient recycling, highlighting how abiotic variation affects potential ecosystem functioning.http://journal.frontiersin.org/article/10.3389/fmicb.2016.01823/ful
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