Comprehensive cross-platform comparison of methods for non-invasive EGFR mutation testing : results of the RING observational trial.

Abstract Several platforms for noninvasive EGFR testing are currently used in the clinical setting with sensitivities ranging from 30% to 100%. Prospective studies evaluating agreement and sources for discordant results remain lacking. Herein, seven methodologies including two next-generation sequencing (NGS)-based methods, three high-sensitivity PCR-based platforms, and two FDA-approved methods were compared using 72 plasma samples, from EGFR-mutant non-small-cell lung cancer (NSCLC) patients progressing on a first-line tyrosine kinase inhibitor (TKI). NGS platforms as well as high-sensitivity PCR-based methodologies showed excellent agreement for EGFR-sensitizing mutations (K = 0.80-0.89) and substantial agreement for T790M testing (K = 0.77 and 0.68, respectively). Mutant allele frequencies (MAFs) obtained by different quantitative methods showed an excellent reproducibility (intraclass correlation coefficients 0.86-0.98). Among other technical factors, discordant calls mostly occurred at mutant allele frequencies (MAFs) ≤ 0.5%. Agreement significantly improved when discarding samples with MAF ≤ 0.5%. EGFR mutations were detected at significantly lower MAFs in patients with brain metastases, suggesting that these patients risk for a false-positive result. Our results support the use of liquid biopsies for noninvasive EGFR testing and highlight the need to systematically report MAFs. Keywords: NGS; circulating free DNA; epidermal growth factor receptor; non-small-cell lung cancer; osimertinib; tyrosine kinase inhibitor

José Bergamín: entre literatura y política

International audienceEste libro es el resultado del Coloquio Internacional «Bergamín: entre literatura y política», organizado por las universidades Paris Ouest Nanterre La Défense y Autònoma de Barcelona. Participan destacados especialistas del escritor español José Bergamín, como Nigel Dennis, a quien rinde homenaje esta publicación. Dieciséis contribuciones conforman este volumen que propone, treinta años después de posarse sobre el poeta la mano de nieve, una reflexión sobre las dos grandes pasiones de su vida: la literatura y la política. A modo de anexo se incluyen las respuestas a la convocatoria «Un silencio por Bergamín», en la que han participado cerca de cuarenta escritores, artistas e investigadores.Cet ouvrage collectif est le résultat du Colloque International « Bergamín: entre literatura y política », organisé par les universités Paris Ouest Nanterre La Défense et Autònoma de Barcelona, avec la participation des meilleurs spécialistes de l’écrivain espagnol José Bergamín, parmi lesquels Nigel Dennis, à qui cette publication rend hommage. L’ensemble des seize contributions recueillies propose, trente ans après la mort de cet important intellectuel du XXe siècle, une réflexion sur les deux grandes passions de sa vie : la littérature et la politique. En annexe, l’hommage « Un silencio por Bergamín » rassemble les contributions d’une quarantaine d’écrivains, artistes et chercheurs