99 research outputs found
Disialo–trisialo bridging of transferrin is due to increased branching and fucosylation of the carbohydrate moiety
Identification of Novel Membrane Structures in Plasmodium falciparum Infected Erythrocytes
Sequential enrichment of sulfated glycans by strong anion-exchange chromatography prior to mass spectrometric measurements
Unraveling the Glyco-Puzzle: Glycan Structure Identification by Capillary Electrophoresis
A Rapid and Simple Method of Purification of Toxoplasma gondii Trophozoites Originating from Tissue Culture for Use in the Indirect Immunofluorescent Antibody Test
The patterns of the complex- and oligomannose-type glycans of uromodulin (Tamm-Horsfall glycoprotein) in the course of pregnancy
Uromodulin was isolated from urine of three pregnant women. Urine of each donor was collected at subsequent stages of their pregnancy and at one month after gestation. Each batch of uromodulin was enzymatically N-deglycosylated and the released N-glycans were isolated, quantified and profiled by high-pH anion-exchange chromatography. In the course of pregnancy no significant changes were detected in the negative charge distribution stemming from sialic acid and sulfate residues on the complex-type carbohydrate chains of uromodulin. Furthermore, no significant changes in the molar ratio between Man6GlcNAc2 and Man7GlcNAc2 were found in the course of pregnancy, only uromodulin from non-pregnant periods showed small differences
The mapping by high-pH anion-exchange chromatography with pulsed amperometric detection and capillary electrophoresis of the carbohydrate moieties of human plasma α1-acid glycoprotein
A strategy for the mapping of N-glycans by high-pH anion-exchange chromatography with pulsed amperometric detection
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