Report on the legal framework governing the use of nutrient rich side streams (NRSS) as biobased fertilisers (BBFs) - EU legislation

Dieser Bericht entstand als Teil des Projektleistung 1.2 des Arbeitspakets 1 im Projekt LEX4BIO, das im Rahmen des EU Forschungs- und Innovationsprogramm „Horizon 2020” gefördert wird (siehe acknowledgement). Er beschreibt das Europäische Recht über Düngeprodukte für die konventionelle und ökologische Landwirtschaft, sowie weitere Europäische Normen, die für die Nutzung nährstoffreicher Nebenströme zur Herstellung biobasierter Düngeprodukte relevant sind. Insbesondere enthält der Bericht Informationen zum Recht des Abfallmanagements, der Qualität von Grund- und Oberflächengewässern, zu Rechtsnormen über Klimaschutz/erneuerbare Energien sowie zu rechtlichen Regelungen aus dem Bereich der Gemeinsamen Agrarpolitik. Die Zusammenstellung soll Interessenvertretern, Wissenschaftlern und allen anderen, die im Bereich „Biobasierte Düngeprodukte“ arbeiten oder sich dafür interessieren, als Handbuch und Referenz dienen. Dieser Bericht ist Teil A der Projektleistung von WP 1, welche aus zwei Teilen bestehen wird. Er wird zu einem späteren Zeitpunkt im Projekt durch Teil B ergänzt werden, der sich mit der nationalen Rechtslage in den EU-Mitgliedsstaaten befasst.This report forms part of Deliverable 1.2 from Work Package 1 in the LEX4BIO project, which received funding from the European Union’s Horizon 2020 research and innovation programme (see acknowledgement). It describes European legislation on fertilising products for conventional and organic farming, as well as other legislation that is relevant in the context of using nutrient-rich sidestreams (NRSS) as biobased fertilisers (BBFs). In particular, legislation on waste management, ground and surface water quality, climate/renewable energy as well as legislation related to the Common Agricultural Policy (CAP) is covered in this report. The report is intended as a handbook and reference for stakeholders, researchers and all others working or interested in the field of biobased fertilisers. This report forms part A of a deliverable from WP1 that will consist of two parts. It will be complemented at a later stage of the project with part B, which will cover national legislation of the European Member States

Nitrous oxide in the surface layer of the tropical North Atlantic Ocean along a west to east transect

Nitrous oxide (N2O) was measured during the first German SOLAS (Surface Ocean – Lower Atmosphere Study) cruise in the tropical North Atlantic Ocean on board R/V Meteor during October/November 2002. About 900 atmospheric and dissolved N2O measurements were performed with a semi-continuous GC-ECD system equipped with a seawater-gas equilibrator. Surface waters along the main transect at 10°N showed no distinct longitudinal gradient. Instead, N2O saturations were highly variable ranging from 97% to 118% (in the Guinea Dome Area, 11°N, 24°W). When approaching the continental shelf of West Africa, N2O surface saturations went up to 113%. N2O saturations in the region of the equatorial upwelling (at 0–1.5°N, 23.5–26°W) were correlated with decreasing sea surface temperatures and showed saturations up to 109%. The overall mean N2O saturation was 104 ± 4% indicating that the tropical North Atlantic Ocean is a net source of atmospheric N2O

North Atlantic production of nitrous oxide in the context of changing atmospheric levels

We use transit time distributions calculated from tracer data together with in situ measurements of N(2)O to estimate the concentration of biologically produced N(2)O ([N(2)O](xs)) and N(2)O production rates in the central North Atlantic Ocean. Our approach to estimation of N(2)O production rates integrates the effects of potentially varying production and decomposition mechanisms along the transport path of a water mass. We find that previously used approaches overestimate the oceanic equilibrium N(2)O concentrations by 8-13% and thus underestimate the strength of N(2)O sources in large parts of the water column. Thus the quantitative characteristics of the [N(2)O](xs)/AOU relationship used as an indicator of nitrification are distorted. We developed a new parameterization of N(2)O production during nitrification depending linearly on AOU and exponentially on temperature and depth, which can be applied to calculate N(2)O production due to nitrification in the entire ocean including oxygen minimum zones

Quantum repeaters and quantum key distribution: analysis of secret key rates

We analyze various prominent quantum repeater protocols in the context of long-distance quantum key distribution. These protocols are the original quantum repeater proposal by Briegel, D\"ur, Cirac and Zoller, the so-called hybrid quantum repeater using optical coherent states dispersively interacting with atomic spin qubits, and the Duan-Lukin-Cirac-Zoller-type repeater using atomic ensembles together with linear optics and, in its most recent extension, heralded qubit amplifiers. For our analysis, we investigate the most important experimental parameters of every repeater component and find their minimally required values for obtaining a nonzero secret key. Additionally, we examine in detail the impact of device imperfections on the final secret key rate and on the optimal number of rounds of distillation when the entangled states are purified right after their initial distribution.Comment: Published versio

Generation of anti-TLR2 intrabody mediating inhibition of macrophage surface TLR2 expression and TLR2-driven cell activation

<p>Abstract</p> <p>Background</p> <p>Toll-like receptor (TLR) 2 is a component of the innate immune system and senses specific pathogen associated molecular patterns (PAMPs) of both microbial and viral origin. Cell activation via TLR2 and other pattern recognition receptors (PRRs) contributes to sepsis pathology and chronic inflammation both relying on overamplification of an immune response. Intracellular antibodies expressed and retained inside the endoplasmatic reticulum (ER-intrabodies) are applied to block translocation of secreted and cell surface molecules from the ER to the cell surface resulting in functional inhibition of the target protein. Here we describe generation and application of a functional anti-TLR2 ER intrabody (αT2ib) which was generated from an antagonistic monoclonal antibody (mAb) towards human and murine TLR2 (T2.5) to inhibit the function of TLR2. αT2ib is a scFv fragment comprising the variable domain of the heavy chain and the variable domain of the light chain of mAb T2.5 linked together by a synthetic (Gly₄Ser)₃amino acid sequence.</p> <p>Results</p> <p>Coexpression of αT2ib and mouse TLR2 in HEK293 cells led to efficient retention and accumulation of TLR2 inside the ER compartment. Co-immunoprecipitation of human TLR2 with αT2ib indicated interaction of αT2ib with its cognate antigen within cells. αT2ib inhibited NF-κB driven reporter gene activation via TLR2 but not through TLR3, TLR4, or TLR9 if coexpressed in HEK293 cells. Co-transfection of human TLR2 with increasing amounts of the expression plasmid encoding αT2ib into HEK293 cells demonstrated high efficiency of the TLR2-αT2ib interaction. The αT2ib open reading frame was integrated into an adenoviral cosmid vector for production of recombinant adenovirus (AdV)-αT2ib. Transduction with AdVαT2ib specifically inhibited TLR2 surface expression of murine RAW264.7 and primary macrophages derived from bone marrow (BMM). Furthermore, TLR2 activation dependent TNFα mRNA accumulation, as well as TNFα translation and release by macrophages were largely abrogated upon transduction of αT2ib. αT2ib was expressed in BMM and splenocytes over 6 days upon systemic infection with AdVαT2ib. Systemic transduction applying AdVαT2ib rendered immune cells largely non-responsive to tripalmitoyl-peptide challenge. Our results show persistent paralysis of TLR2 activity and thus inhibition of immune activation.</p> <p>Conclusion</p> <p>The generated anti-TLR2 scFv intrabody inhibits specifically and very efficiently TLR2 ligand-driven cell activation <it>in vitro </it>and <it>ex vivo</it>. This indicates a therapeutic potential of αT2ib in microbial or viral infections.</p

Understanding European Regional Diversity - Lessons learned from Case Studies

The content of this report is a deliverable to the FP 7 project RUFUS (Rural future Networks) concerning the case studies made within the project. As a deliverable in a EU framework project it reports extensively on the methods and empirical data collected in the project’s case studies. The work has as an overarching motive to translate research findings into implications that are relevant for policy makers in the EU. The conclusions from the case studies are therefore of two types – the findings made and the implications they might give for policy making within the field of rural development