593 research outputs found
Das HDW-System der Genaufertigung: Beherrschung der Schweißschrumpfungen – ein wesentlicher Faktor für Wirtschaftlichkeit und Qualität
Die Genaufertigung von Schweißkonstruktionen ist ein wichtiger Wettbewerbsfaktor in der Werftindustrie. In diesem Beitrag werden Ziele, Werkzeuge, Technologie und Meßtechnik der Genaufertigung erläutert
REACTION AND PERFORMANCE TIME OF TAEKWONDO TOP-ATHLETES DEMONSTRATING THE BALDUNG-CHAGI
The purpose of this study was to investigate two factors for success in taekwondo competition, the simple reaction time (RT) - beginning with an external signal and ending with a shoulder, hip or ankle movement – and the performance time (PT) – starting of the Baldung-chagi till hitting the target. Subjects were the top 9 athletes (6 ♂, 3 ♀) from the German National Team. Movements were recorded by 3 infrared cameras of LUKOtronic active marker Motion Capturing System. Seven markers were used, five on the subject (ankle, heel, hip, shoulder, wrist of the kicking side), one trigger marker at the floor, one at the hand-mitt. The average value for the ankle RT is 0.34 sec., 0.26 sec. for hip RT, 0.23 sec. for shoulder RT and 0.31 sec, for PT. A high ankle RT variation, in the range of 0.26 to 0.54 sec, indicates that reducing this variation though training arrangements could improves the probability for success
Recursive algorithm for the best approximate solution of linear equations with applications to system identification and state estimation.
http://www.archive.org/details/recursivealgorit00wer
Improved Tet-responsive promoters with minimized background expression
<p>Abstract</p> <p>Background</p> <p>The performance of the tetracycline controlled transcriptional activation system (Tet system) depends critically on the choice of minimal promoters. They are indispensable to warrant low expression levels with the system turned "off". On the other hand, they must support high level of gene expression in the "on"-state.</p> <p>Results</p> <p>In this study, we systematically modified the widely used Cytomegalovirus (CMV) minimal promoter to further minimize background expression, resulting in an improved dynamic expression range. Using both plasmid-based and retroviral gene delivery, our analysis revealed that especially background expression levels could be significantly reduced when compared to previously established "standard" promoter designs. Our results also demonstrate the possibility to fine-tune expression levels in non-clonal cell populations. They also imply differences regarding the requirements for tight regulation and high level induction between transient and stable gene transfer systems.</p> <p>Conclusions</p> <p>Until now, our understanding of mammalian transcriptional regulation including promoter architecture is limited. Nevertheless, the partly empirical modification of <it>cis</it>-elements as shown in this study can lead to the specific improvement of the performance of minimal promoters. The novel composite Ptet promoters introduced here will further expand the utility of the Tet system.</p
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