A randomized comparison of HBP versus RVP: Effect on left ventricular function and biomarkers of collagen metabolism

Background: Right ventricular pacing (RVP) can result in pacing-induced cardiomyopathy (PICM). It is unknown whether specific biomarkers reflect differences between His bundle pacing (HBP) and RVP and predict a decrease in left ventricular function during RVP. Aims: To compare the effect of HBP and RVP on the LV ejection fraction (LVEF) and to study how they affect serum markers of collagen metabolism. Methods: Ninety-two high-risk PICM patients were randomized to HBP or RVP. Their clinical characteristics, echocardiography, and serum levels of TGF-β1, MMP-9, ST2-IL, TIMP-1, and Gal-3 were studied before and six months after pacemaker implantation. Results: Fifty-three patients were randomized to HBP and 39 patients to RVP. HBP failed in 10 patients, which crossed over to the RVP group. Patients with RVP had significantly lower LVEF compared to HBP after six months of pacing (−5% and −4% in as-treated and intention-to-treat analysis, respectively). Levels of TGF-β1 after 6 months were lower in HBP than RVP (mean difference −6 ng/ml; P = 0.009) and preimplant Gal-3 and ST2-IL levels were higher in RVP patients with a decline in the LVEF ≥ 5% compared to those with a decline of < 5% (mean difference 3 ng/ml and 8 ng/ml; P = 0.02 for both). Conclusion: In high-risk PICM patients, HBP was superior to RVP in providing more physiological ventricular function, as reflected by higher LVEF and lower levels of TGF-β1. Among RVP patients, LVEF declined more in those with higher baseline Gal-3 and ST2-IL levels than those with lower levels

Protests against the Bata company: Contribution to the study of anti-industrial processes in the kingdom of serbs, croats and Slovenes/Yugoslavia

The paper presents an analysis of the anti-industrial processes in the Kingdom of Serbs, Croats and Slovenes, later the Kingdom of Yugoslavia. In this context, special attention was paid to the protests against the Bata Company, which by its arrival suppressed domestic industrial and artisan production in the leather industry, i.e. deepened the unfavorable social position of the population, existentially dependent on domestic footwear production. The Bata Company in Yugoslavia was founded in 1920 and until the end of the decade the retail network grew, but there was still no production, as the footwear and accessories were imported from Czechoslovakia. After the establishment of the factory in Borovo in 1931, Bata became the dominant shoe producer in the entire country and covered almost 90 per cent of domestic production and sales. However, during its expansion, the Company was exposed to growing protests from domestic producers and small shoemakers. Those protests lasted almost a decade, peaking at the turn of 1935/1936, by organizing mass demonstrations, which occasionally grew into violent incidents. These protests caused the adoption of several measures by the Yugoslav authorities, which limited the business of the company but did not adopt legislation that would take the form of a law, the so-called Lex Bata, which were adopted in Switzerland and France. After that peak, the protests slowly subsided, so that at the end of the decade, Bata completely took over the market of the Kingdom of Yugoslavia. © 2021 Institut za savremenu istoriju. All rights reserved

The early decades of the Bata Shoe Company in India: From establishment to economic and social integration

The study presents a historical and sociological interpretation of the events that marked the gradual integration of the Bata Company into the Indian economy and society from the mid-1920s to the early 1960s. Within this context, in addition to the general economic, political and cultural developments, particular attention has been devoted to the everyday life of Indian and Czech workers in the Bata company town of Batanagar. The study is based on a comparative-historical analysis of available archival sources and a secondary analysis of the relevant academic literature. The results of the research indicate that during this period, Bata was forced to adapt continuously to the cultural specifics of Indian society, that is, the process of its integration into the Indian economy and society had pronounced glocal characteristics

The social welfare system in Bata company towns (1920s-1950s): Between transnational vision and local settings

In the early twentieth century, the Bata company became one of the largest shoe manufacturers in the world, and an emblematic icon of family capitalism. This paper presents an overview of the social welfare system developed by the firm, first in its hometown of Zlin (Moravia) and then in more than thirty company towns founded in Czechoslovakia, Europe, and other continents from the 1920s to the 1950s. It shows how the initial model provided by the city of Zlin took different forms after being exported to other settlements, and aims to identify the causes of this divergence. Following a transnational perspective, this research contributes to a better understanding of how policies, models, and practices transferred around the world by multinational companies can be reshaped according to national and local contexts

Photoactive and non-hazardous kaolinite/ZnO nanocomposite: Characterization and reproducibility of the preparation process

Photoactive and non-hazardous kaolinite/ZnO nanocomposite with 50 wt.% of ZnO nanoparticles was prepared using simple and cheap hydrothermal method. The resulting solid phase was separated by decantation, and dried at 105 degrees C. Calcination of the nanocomposites at 600 degrees C led to the kaolinite-metakaolinite phase transformation, to further growth of ZnO crystallites, and to significant increase in photodegradation activity. Whereas, for the several applications, e.g., in brake industry, the larger amount of composites is needed, thus, the evaluation of the reproducibility of preparation process is one of the crucial parameter. Prepared nanocomposites were deeply characterized by using X-ray fluorescence spectroscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and Rietveld quantitative phase analysis. Photodegradation activity was evaluated by the discoloration of Acid Orange 7 aqueous solution under-UV irradiation. All used analytical techniques and methods confirm the reproducibility of the preparation process and as well that ZnO nanoparticles are anchored tightly on the clay surface which prevents the release to the environment.Web of Science1952868286

Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein

Phosducin (Pdc), a highly conserved phosphoprotein involved in the regulation of retinal phototransduction cascade, transcriptional control, and modulation of blood pressure, is controlled in a phosphorylation-dependent manner, including the binding to the 14-3-3 protein. However, the molecular mechanism of this regulation is largely unknown. Here, the solution structure of Pdc and its interaction with the 14-3-3 protein were investigated using small angle x-ray scattering, time-resolved fluorescence spectroscopy, and hydrogen-deuterium exchange coupled to mass spectrometry. The 14-3-3 protein dimer interacts with Pdc using surfaces both inside and outside its central channel. The N-terminal domain of Pdc, where both phosphorylation sites and the 14-3-3-binding motifs are located, is an intrinsically disordered protein that reduces its flexibility in several regions without undergoing dramatic disorder-to-order transition upon binding to 14-3-3. Our data also indicate that the C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer through the region involved in G$_t$βγ binding. In conclusion, we show that the 14-3-3 protein interacts with and sterically occludes both the N- and C-terminal Gtβγ binding interfaces of phosphorylated Pdc, thus providing a mechanistic explanation for the 14-3-3-dependent inhibition of Pdc function

Structural Insight into the 14-3-3 Protein-dependent Inhibition of Protein Kinase ASK1 (Apoptosis Signal-regulating kinase 1)

Apoptosis signal-regulating kinase 1 (ASK1, also known as MAP3K5), a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family, regulates diverse physiological processes. The activity of ASK1 is triggered by various stress stimuli and is involved in the pathogenesis of cancer, neurodegeneration, inflammation, and diabetes. ASK1 forms a high molecular mass complex whose activity is, under non-stress conditions, suppressed through interaction with thioredoxin and the scaffolding protein 14-3-3. The 14-3-3 protein binds to the phosphorylated Ser-966 motif downstream of the ASK1 kinase domain. The role of 14-3-3 in the inhibition of ASK1 has yet to be elucidated. In this study we performed structural analysis of the complex between the ASK1 kinase domain phosphorylated at Ser-966 (pASK1-CD) and the 14-3-3ζ protein. Small angle x-ray scattering (SAXS) measurements and chemical cross-linking revealed that the pASK1-CD·14-3-3ζ complex is dynamic and conformationally heterogeneous. In addition, structural analysis coupled with the results of phosphorus NMR and time-resolved tryptophan fluorescence measurements suggest that 14-3-3ζ interacts with the kinase domain of ASK1 in close proximity to its active site, thus indicating this interaction might block its accessibility and/or affect its conformation