The Revised Systematics and Paleoecology of the Devonian Stemless Crinoid Genus \u3ci\u3eEdriocrinus\u3c/i\u3e Hall, 1858

New morphological observations of Edriocrinus Hall, 1858, enable a modern, holistic view of this unusual crinoid genus, previously included in the Superorder Flexibilia (Zittel, 1895) Wright et al., 2017. Re-analysis of Edriocrinus suggests that the genus should now be assigned to the Order ‘Dendrocrinida’ within the Magnorder Eucladida Wright, 2017 based on the five infrabasals, single radianal in the cup, absent anal sac, and non-pinnulate arms with rectangular uniserial brachials. Moreover, examination of the slight variations separating the current 14 Edriocrinus species indicates that these “species” are likely ecophenotypes. The current Edriocrinus species are revised based on firmly bound calyx plates; five high infrabasals; lack of patelloid processes; straight muscular radial articulations; brachials that are free above the radials; and muscular articulation between brachials and synonymized to four species, E. pocilliformis, E. sacculus, E. pyriformis, and E. dispansus. Edriocrinus is restricted to a ~25 Myr interval in the Early and Middle Devonian, a time of global eustatic and tectonic disruption, when its stemlessness provided an adaptive advantage throughout environments in the Old World and Eastern Americas realms. These realms were in subtropical to warm temperate climatic zones that encompassed the Rheic Ocean between 25°and 35⁰ south latitude. Edriocrinus is found in formerly adjacent parts of east-central North America, south-central Europe, southern England, and northern Africa. The genus persisted in south-central Europe until the Chotec Event in early Eifelian time and in North America until the Bakoven Event in mid-Eifelian time, when episodes of transgression and anoxia led to genus extinction

Proceedings of the 1997 Annual Meeting Association of American Law School Sections on Employment Discrimination Law and Alternative Dispute Resolution

The following is an edited transcript of the proceedings of the joint meeting of the Employment Discrimination Law and Alternative Dispute Resolution Sections at the AALS Annual Meeting, Washington, D.C., January 7, 1997

Methods for the evaluation of the Jamie Oliver Ministry of Food program, Australia.

BACKGROUND: Community-based programs aimed at improving cooking skills, cooking confidence and individual eating behaviours have grown in number over the past two decades. Whilst some evidence exists to support their effectiveness, only small behavioural changes have been reported and limitations in study design may have impacted on results.This paper describes the first evaluation of the Jamie Oliver Ministry of Food Program (JMoF) Australia, in Ipswich, Queensland. JMoF Australia is a community-based cooking skills program open to the general public consisting of 1.5 hour classes weekly over a 10 week period, based on the program of the same name originating in the United Kingdom. METHODS/DESIGN: A mixed methods study design is proposed. Given the programmatic implementation of JMoF in Ipswich, the quantitative study is a non-randomised, pre-post design comparing participants undergoing the program with a wait-list control group. There will be two primary outcome measures: (i) change in cooking confidence (self-efficacy) and (ii) change in self-reported mean vegetable intake (serves per day). Secondary outcome measures will include change in individual cooking and eating behaviours and psycho-social measures such as social connectedness and self-esteem. Repeated measures will be collected at baseline, program completion (10 weeks) and 6 months follow up from program completion. A sample of 250 participants per group will be recruited for the evaluation to detect a mean change of 0.5 serves a day of vegetables at 80% power (0.5% significance level). Data analysis will assess the magnitude of change of these variables both within and between groups and use sub group analysis to explore the relationships between socio-demographic characteristics and outcomes.The qualitative study will be a longitudinal design consisting of semi-structured interviews with approximately 10-15 participants conducted at successive time points. An inductive thematic analysis will be conducted to explore social, attitudinal and behavioural changes experienced by program participants. DISCUSSION: This evaluation will contribute to the evidence of whether cooking programs work in terms of improving health and wellbeing and the underlying mechanisms which may lead to positive behaviour change. TRIAL REGISTRATION: Australian and New Zealand Trial registration number: ACTRN12611001209987

Medical genetics and epigenetics of telomerase

Telomerase is a specialized reverse transcriptase that extends and maintains the terminal ends of chromosomes, or telomeres. Since its discovery in 1985 by Nobel Laureates Elizabeth Blackburn and Carol Greider, thousands of articles have emerged detailing its significance in telomere function and cell survival. This review provides a current assessment on the importance of telomerase regulation and relates it in terms of medical genetics. In this review, we discuss the recent findings on telomerase regulation, focusing on epigenetics and non-coding RNAs regulation of telomerase, such as microRNAs and the recently discovered telomeric-repeat containing RNA transcripts. Human genetic disorders that develop due to mutations in telomerase subunits, the role of single nucleotide polymorphisms in genes encoding telomerase components and diseases as a result of telomerase regulation going awry are also discussed. Continual investigation of the complex regulation of telomerase will further our insight into the use of controlling telomerase activity in medicine

The Passive Journalist: How sources dominate the local news

This study explores which sources are “making” local news and whether these sources are simply indicating the type of news that appears, or are shaping newspaper coverage. It provides an empirical record of the extent to which sources are able to dominate news coverage from which future trends in local journalism can be measured. The type and number of sources used in 2979 sampled news stories in four West Yorkshire papers, representing the three main proprietors of local newspapers in the United Kingdom, were recorded for one month and revealed the relatively narrow range of routine sources; 76 per cent of articles cited only a single source. The analysis indicates that journalists are relying less on their readers for news, and that stories of little consequence are being elevated to significant positions, or are filling news pages at the expense of more important stories. Additionally, the reliance on a single source means that alternative views and perspectives relevant to the readership are being overlooked. Journalists are becoming more passive, mere processors of one-sided information or bland copy dictated by sources. These trends indicate poor journalistic standards and may be exacerbating declining local newspaper sales

Pervasive transcription of a herpesvirus genome generates functionally important RNAs

ABSTRACT Pervasive transcription is observed in a wide range of organisms, including humans, mice, and viruses, but the functional significance of the resulting transcripts remains uncertain. Current genetic approaches are often limited by their emphasis on protein-coding open reading frames (ORFs). We previously identified extensive pervasive transcription from the murine gammaherpesvirus 68 (MHV68) genome outside known ORFs and antisense to known genes (termed expressed genomic regions [EGRs]). Similar antisense transcripts have been identified in many other herpesviruses, including Kaposi’s sarcoma-associated herpesvirus and human and murine cytomegalovirus. Despite their prevalence, whether these RNAs have any functional importance in the viral life cycle is unknown, and one interpretation is that these are merely “noise” generated by functionally unimportant transcriptional events. To determine whether pervasive transcription of a herpesvirus genome generates RNA molecules that are functionally important, we used a strand-specific functional approach to target transcripts from thirteen EGRs in MHV68. We found that targeting transcripts from six EGRs reduced viral protein expression, proving that pervasive transcription can generate functionally important RNAs. We characterized transcripts emanating from EGRs 26 and 27 in detail using several methods, including RNA sequencing, and identified several novel polyadenylated transcripts that were enriched in the nuclei of infected cells. These data provide the first evidence of the functional importance of regions of pervasive transcription emanating from MHV68 EGRs. Therefore, studies utilizing mutation of a herpesvirus genome must account for possible effects on RNAs generated by pervasive transcription. IMPORTANCE The fact that pervasive transcription produces functionally important RNAs has profound implications for design and interpretation of genetic studies in herpesviruses, since such studies often involve mutating both strands of the genome. This is a common potential problem; for example, a conservative estimate is that there are an additional 73,000 nucleotides transcribed antisense to annotated ORFs from the 119,450-bp MHV68 genome. Recognizing the importance of considering the function of each strand of the viral genome independently, we used strand-specific approaches to identify six regions of the genome encoding transcripts that promoted viral protein expression. For two of these regions, we mapped novel transcripts and determined that targeting transcripts from these regions reduced viral replication and the expression of other viral genes. This is the first description of a function for these RNAs and suggests that novel transcripts emanating from regions of pervasive transcription are critical for the viral life cycle

A typology for world electricity mix: Application for inventories in Consequential LCA (CLCA)

Over the past two decades, the integration of environmental concerns into decision making has been gaining prominence both at national and global levels. Sustainable development now factors into policy design as well as industrial technological choices. For this purpose, Life Cycle Assessment (LCA)–which evaluates environmental impacts of products, processes and services through their complete life cycle–is considered a crucial tool to support the integration of environmental sustainability into decision making. In particular, Consequential LCA (CLCA) has emerged as an approach to assess consequences of change, considering both direct and indirect impacts of changes. Currently, no long-term datasets of Consequential Life Cycle Inventories (CLCI) are available, particularly in the case of electricity production mixes. A first and fundamental step to begin filling this gap is to make available data on national level greenhouse gas emissions from electricity and create a typology of electricity production mixes to support policy making. The proposed typology is based on the analysis of the composition of electricity production mixes of 91 countries producing more than 10 TWh in 2012, on the one hand, and of their calculated greenhouse gas (GHG) emissions (in gCO2eq/kWh) from LCA using IPCC 2013 data, on the other hand. All types of primary energy resources are considered, and some are grouped according to similarities in their emissions intensities. Using graphical observations of these two characteristics and a boundary definition, we create a 4-group typology for GHG emissions per kWh, i.e., very low (0–37 gCO2eq/kWh), low (37–300 gCO2eq/kWh), mean (300–600 gCO2eq/kWh) and high (>600 gCO2eq/kWh). The typology is based on the general characteristics of the electric power generation fleet, corresponding respectively to power systems heavy on hydraulic and/or nuclear power with the remainder of the fleet dominated by renewables; hydraulic and/or nuclear power combined with a diversified mix; gas with a diversified mix; coal, oil and predominantly fossils. This typology describes the general tendencies of the electricity mix and, over time, it can help point to ways in which countries can transition between groups. Further steps should be devoted to the development of indicators taking into account grid interconnection, energy sector resilience in the quest for a mix optimum