19 research outputs found
Avaliação ecológica råpida da fauna de vespas (Hymenoptera: Aculeata) do Parque Nacional da Serra do Divisor, Acre, Brasil
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Distribution, abundance and persistence of species of Orasema (Hym: Eucharitidae) parasitic on fire ants in South America
Distribution, abundance and persistence of species of Orasema (Hym: Eucharitidae) parasitic on fire ants in South America
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Distribution, abundance and persistence of species of Orasema (Hym: Eucharitidae) parasitic on fire ants in South America
Inverse dispersal patterns in a group of ant parasitoids (Hymenoptera: Eucharitidae: Oraseminae) and their ant hosts
Neotropical ant parasitoids (Hymenoptera: Eucharitidae): interpreting taxonomy, phylogeny and divergent morphologies
Tissue microarray (TMA) use in post mortem neuropathology
Background
Tissue microarrays (TMAs), where each block (and thus section) contains multiple tissue cores from multiple blocks potentially allow more efficient use of tissue, reagents and time in neuropathology.
New Method
The relationship between data from TMA cores and whole sections was investigated using âvirtualâ TMA cores. This involved quantitative assessments of microglial pathology in white matter lesions and motor neuron disease, alongside qualitative TDP-43 inclusion status in motor neuron disease cases. Following this, a protocol was developed for TMA construction.
Results
For microglial pathology we found good concordance between virtual cores and whole sections for volume density using one 1.75âmm core (equivalent to a 2âmm core after accounting for peripheral tissue loss). More sophisticated microglial cell size and measures required two cores. Qualitative results of pTDP-43 pathology showed use of one 1.75âmm core gave a 100% sensitivity and specificity within grey matter, and 88.3% sensitivity and 100% specificity within white matter. A method of producing the TMAs was suitable for immunohistochemistry both manually and by autostainer, with the minimal core loss from the microscope slide.
Comparison with Existing Methods
TMAs have been used infrequently in post mortem neuropathology research. However, we believe TMAs give comparable tissue assessment results and can be constructed, sectioned and stained with relative ease.
Conclusions
We found TMAs could be used to assess both quantitative (microglial pathology) and qualitative pathology (TDP-43 proteinopathy) with greatly reduced quantities of tissue, time and reagents. These could be used for further work to improve data acquisition efficiency
Down the rabbit hole: A phylogenetic hypothesis for the Chalcidoidea
International audienc