6 research outputs found
Kaiso (ZBTB33) subcellular partitioning functionally links LC3A/B, the tumor microenvironment, and breast cancer survival
The use of digital pathology for the histomorphologic profiling of pathological specimens is expanding the precision and specificity of quantitative tissue analysis at an unprecedented scale; thus, enabling the discovery of new and functionally relevant histological features of both predictive and prognostic significance. In this study, we apply quantitative automated image processing and computational methods to profile the subcellular distribution of the multi-functional transcriptional regulator, Kaiso (ZBTB33), in the tumors of a large racially diverse breast cancer cohort from a designated health disparities region in the United States. Multiplex multivariate analysis of the association of Kaiso’s subcellular distribution with other breast cancer biomarkers reveals novel functional and predictive linkages between Kaiso and the autophagy-related proteins, LC3A/B, that are associated with features of the tumor immune microenvironment, survival, and race. These findings identify effective modalities of Kaiso biomarker assessment and uncover unanticipated insights into Kaiso’s role in breast cancer progression.Fil: Singhal, Sandeep K.. North Dakota State University; Estados UnidosFil: Byun, Jung S.. National Institutes of Health; Estados UnidosFil: Park, Samson. National Institutes of Health; Estados UnidosFil: Yan, Tingfen. National Institutes of Health; Estados UnidosFil: Yancey, Ryan. Columbia University; Estados UnidosFil: Caban, Ambar. Columbia University; Estados UnidosFil: Hernandez, Sara Gil. National Institutes of Health; Estados UnidosFil: Hewitt, Stephen M.. U.S. Department of Health & Human Services. National Institute of Health. National Cancer Institute; Estados UnidosFil: Boisvert, Heike. Ultivue, Inc; Reino UnidoFil: Hennek, Stephanie. Ultivue Inc.; Reino UnidoFil: Bobrow, Mark. Ultivue Inc.; Reino UnidoFil: Ahmed, Md Shakir Uddin. Tuskegee University; Estados UnidosFil: White, Jason. Tuskegee University; Estados UnidosFil: Yates, Clayton. Tuskegee University; Estados UnidosFil: Aukerman, Andrew. Columbia University; Estados UnidosFil: Vanguri, Rami. Columbia University; Estados UnidosFil: Bareja, Rohan. Columbia University; Estados UnidosFil: Lenci, Romina. Columbia University; Estados UnidosFil: FarrĂ©, Paula LucĂa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de BiologĂa y Medicina Experimental. FundaciĂłn de Instituto de BiologĂa y Medicina Experimental. Instituto de BiologĂa y Medicina Experimental; ArgentinaFil: de Siervi, Adriana. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Instituto de BiologĂa y Medicina Experimental. FundaciĂłn de Instituto de BiologĂa y Medicina Experimental. Instituto de BiologĂa y Medicina Experimental; ArgentinaFil: Nápoles, Anna MarĂa. National Institutes of Health; Estados UnidosFil: Vohra, Nasreen. East Carolina University; Estados UnidosFil: Gardner, Kevin. Columbia University; Estados Unido
In-Situ Probe of Gate Dielectric-Semiconductor Interfacial Order in Organic Transistors: Origin and Control of Large Performance Sensitivities
Organic thin film transistor (OTFT) performance is highly
materials
interface-dependent, and dramatic performance enhancements can be
achieved by properly modifying the semiconductor/gate dielectric interface.
However, the origin of these effects is not well understood, as this
is a classic “buried interface” problem that has traditionally
been difficult to address. Here we address the question of how <i>n</i>-octadecylsilane (OTS)–derived self-assembled monolayers
(SAMs) on Si/SiO<sub>2</sub> gate dielectrics affect the OTFT performance
of the archetypical small-molecule p-type semiconductors P-BTDT (phenylbenzoÂ[<i>d</i>,<i>d</i>]ÂthienoÂ[3,2-<i>b</i>;4,5-<i>b</i>]Âdithiophene) and pentacene using combined in situ sum
frequency generation spectroscopy, atomic force microscopy, and grazing
incidence and reflectance X-ray scattering. The molecular order and
orientation of the OTFT components at the dielectric/semiconductor
interface is probed as a function of SAM growth mode in order to understand
how this impacts the overlying semiconductor growth mode, packing,
crystallinity, and carrier mobility, and hence, transistor performance.
This understanding, using a new, humidity-specific growth procedure,
leads to a reproducible, scalable process for highly ordered OTS SAMs,
which in turn nucleates highly ordered p-type semiconductor film growth,
and optimizes OTFT performance. Surprisingly, the combined data reveal
that while SAM molecular order dramatically impacts semiconductor
crystalline domain size and carrier mobility, <i>it does not
significantly influence the local orientation of the overlying organic
semiconductor molecules</i>