DEVELOPMENT OF VIRTUAL EVENT MARKETING

In the last few years the Pichia pastoris expression system has been gaining more and more interest for the expression of recombinant proteins. Many groups have employed fermentation technology in their investigations because the system is fairly easy to scale up and suitable for the production in the milligram to gram range. A large number of heterologous proteins from different sources has been expressed, but the fermentation process technology has been investigated to a lesser extent. A large number of fermentations are carried out in standard bioreactors that may be insufficiently equipped to meet the demands of high-cell-density fermentations of methylotrophic yeasts. In particular, the lack of on-line methanol analysis leads to fermentation protocols that may impair the optimal expression of the desired products. We have used a commercially available methanol sensor to investigate in detail the effects of supplementary glycerol feeding while maintaining a constant methanol concentration during the induction of a Mut+ strain of Pichia pastoris. Specific glycerol feed rates in the range of 38-4.2 mg × g(exp -1) × h(exp -1) (mg glycerol per gram fresh weight per hour) were investigated. Expression of the recombinant scFv antibody fragment was only observed at specific feed rates below 6 mg × g(exp -1) × h(exp -1). At low specific feed rates, growth was even lower than with methanol as the sole carbon source and the harvest expression level of the scFv was only half of that found in the control fermentation. These results show that glycerol inhibits expression driven by the AOX1 promoter even at extremely limited availability and demonstrate the benefits of on-line methanol control in Pichia fermentation research

Speech Communication

Contains three reports on research facilities and systems.U. S. Air Force Cambridge Research Laboratories, Office of Aerospace Research, under Contract F19628-69-C-0044National Institutes of Health (Grant 2 ROl NB-04332-06

Law Library Consortium Data Base Components and Standards Study Group Report

The Data Base Components and Standards Committee of the Law Library Consortium recommends the establishment of a national law data base to meet the multi-faceted needs of the legal community for legal and law-related information. The scope of the Report includes bibliographic description and control, as well as subject and full-text access to Anglo-American, foreign, comparative, and international law materials in monographs, serials, non-book media including audio-visual and computerized information, computerassisted instruction, confidential data control and resource persons. Standardsf or inputting information are suggested

Law Library Consortium Data Base Components and Standards Study Group Report

The Data Base Components and Standards Committee of the Law Library Consortium recommends the establishment of a national law data base to meet the multi-faceted needs of the legal community for legal and law-related information. The scope of the Report includes bibliographic description and control, as well as subject and full-text access to Anglo-American, foreign, comparative, and international law materials in monographs, serials, non-book media including audio-visual and computerized information, computerassisted instruction, confidential data control and resource persons. Standardsf or inputting information are suggested

Charge order from structured coupling in VSe₂

Charge order -- ubiquitous among correlated materials -- is customarily described purely as an instability of the electronic structure. However, the resulting theoretical predictions often do not match high-resolution experimental data. A pertinent case is 1T-VSe2, whose single-band Fermi surface and weak-coupling nature make it qualitatively similar to the Peierls model underlying the traditional approach. Despite this, its Fermi surface is poorly nested, the thermal evolution of its charge density wave (CDW) ordering vectors displays an unexpected jump, and the CDW gap itself evades detection in direct probes of the electronic structure. We demonstrate that the thermal variation of the CDW vectors is naturally reproduced by the electronic susceptibility when incorporating a structured, momentum-dependent electron-phonon coupling, while the evasive CDW gap presents itself as a localized suppression of spectral weight centered above the Fermi level. Our results showcase the general utility of incorporating a structured coupling in the description of charge ordered materials, including those that appear unconventional

Speech Communication

Contains reports on three research projects.National Institutes of Health (Grant 5 RO1 NS04332-13)National Institutes of Health (Grant 1 T32 NS07040-01)Joint Services Electronics Program (Contract DAAB07-75-C-1346

The differential effects of plasma from two groups of clinically similar schizophrenic patients on learning behavior in rats

The effects of plasma from normal subjects and chronic schizophrenic patients were determined on the rate of learning a pole jump response in rats. The animals were trained to a buzzer CS and electroshock US. Mean trials to a 90 per cent avoidance criterion were determined using a 100 trial cut off. Plasma and other control solutions were given intraperitoneally in the morning and the procedure repeated in the afternoon with separate groups of rats. Test trials were initiated five minutes after injection. It was found that all plasma, in contrast to saline, produced an increase in the number of trials to learn the avoidance response. No difference in behavioral effects was noted comparing the plasma of all chronic schizophrenics to normals. However, within the chronic schizophrenic population there are subgroups whose plasma was differentially effective in reducing rate of learning. These results are preliminary and subject to continued experimentation. They are presented here to make others awake of the need to consider the existence of possible biologic subgroupings of schizophrenia in future investigations of the plasma-behavior interaction.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33404/1/0000805.pd

Speech Communication

Contains reports on four research projects.National Institutes of Health (Grant 5 ROl NB04332-08)U.S. Air Force Cambridge Research Laboratories, Office of Aerospace Research, under Contract F19628-69-C-004

Carotenoid Production by Corynebacterium: The Workhorse of Industrial Amino Acid Production as Host for Production of a Broad Spectrum of C40 and C50 Carotenoids

Corynebacterium glutamicum is used as a workhorse of industrial biotechnology for more than 60 years since its discovery as a natural glutamate producer in the 1950s. Nowadays, L-glutamate and L-lysine are being produced with this GRAS organism in the million-ton scale every year for the food and feed markets, respectively. Sequencing of the genome and establishment of a genetic toolbox boosted metabolic engineering of this host for a broad range of industrially relevant compounds ranging from bulk chemicals to high-value products. Carotenoids, the colourful representatives of terpenoids, are high-value compounds whose bio-based production is on the rise. Since C. glutamicum is a natural producer of the rare C50 carotenoid decaprenoxanthin, this organism is well suited to establish terpenoid-overproducing platform strains with the help of metabolic engineering strategies. In this work, the carotenogenic background of C. glutamicum and the metabolic engineering strategies for the generation of carotenoid-overproducing strains are depicted