58 research outputs found
J. C. D. Clark β The Language of Liberty, 1660-1832: Political Discourse and Social Dynamics in the Anglo-American World
The Last Populist- George Washington Armstrong and the Texas Gubernatorial Election of 1932, and the \u27Zionist\u27 Threat to Liberty and Constitutional Government
Chronicles of Oklahoma
Article discusses the Works Progress Administration Federal Music Project instituted as one of the relief programs of the New Deal to provide employment to struggling musicians during the Great Depression. Kenneth E. Hendrickson, Jr. investigates its success as well as the difficulties faced by Dean Richardson, Oklahoma state director of the program
Faculty-Librarian Micro-Level Collaboration in an Online Graduate History Course
This paper describes a micro-level faculty-librarian collaboration implemented at the authors' state university to address students' information literacy deficiencies in a graduate-level history research methods course. The setting, implementation, and evolution of the partnership are described in detail to suggest a model for other instructors. Additionally, consideration is given to issues of working in an online course environment and the benefits of micro- versus macro-level librarian support. Consideration is given to future steps for strengthening the partnership and measuring its impact on student outcomes.Librar
Phase change dynamics and 2-dimensional 4-bit memory in Ge2Sb2Te5 via telecom-band encoding
As modern computing gets continuously pushed up against the von Neumann
Bottleneck -- limiting the ultimate speeds for data transfer and computation --
new computing methods are needed in order to bypass this issue and keep our
computer's evolution moving forward, such as hybrid computing with an optical
co-processor, all-optical computing, or photonic neuromorphic computing. In any
of these protocols, we require an optical memory: either a
multilevel/accumulator memory, or a computational memory. Here, we propose and
demonstrate a 2-dimensional 4-bit fully optical non-volatile memory using
Ge2Sb2Te5 (GST) phase change materials, with encoding via a 1550 nm laser.
Using the telecom-band laser, we are able to reach deeper into the material due
to the low-loss nature of GST at this wavelength range, hence increasing the
number of optical write/read levels compared to previous demonstrations, while
simultaneously staying within acceptable read/write energies. We verify our
design and experimental results via rigorous numerical simulations based on
finite element and nucleation theory, and we successfully write and read a
string of characters using direct hexadecimal encoding
Development of a Tetrameric Streptavidin Mutein with Reversible Biotin Binding Capability: Engineering a Mobile Loop as an Exit Door for Biotin
A novel form of tetrameric streptavidin has been engineered to have reversible biotin binding capability. In wild-type streptavidin, loop3β4 functions as a lid for the entry and exit of biotin. When biotin is bound, interactions between biotin and key residues in loop3β4 keep this lid in the closed state. In the engineered mutein, a second biotin exit door is created by changing the amino acid sequence of loop7β8. This door is mobile even in the presence of the bound biotin and can facilitate the release of biotin from the mutein. Since loop7β8 is involved in subunit interactions, alteration of this loop in the engineered mutein results in an 11Β° rotation between the two dimers in reference to wild-type streptavidin. The tetrameric state of the engineered mutein is stabilized by a H127C mutation, which leads to the formation of inter-subunit disulfide bonds. The biotin binding kinetic parameters (koff of 4.28Γ10β4 sβ1 and Kd of 1.9Γ10β8 M) make this engineered mutein a superb affinity agent for the purification of biotinylated biomolecules. Affinity matrices can be regenerated using gentle procedures, and regenerated matrices can be reused at least ten times without any observable reduction in binding capacity. With the combination of both the engineered mutein and wild-type streptavidin, biotinylated biomolecules can easily be affinity purified to high purity and immobilized to desirable platforms without any leakage concerns. Other potential biotechnological applications, such as development of an automated high-throughput protein purification system, are feasible
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