Correction:How the COVID-19 pandemic highlights the necessity of animal research (vol 30, pg R1014, 2020)

(Current Biology 30, R1014–R1018; September 21, 2020) As a result of an author oversight in the originally published version of this article, a number of errors were introduced in the author list and affiliations. First, the middle initials were omitted from the names of several authors. Second, the surname of Dr. van Dam was mistakenly written as “Dam.” Third, the first name of author Bernhard Englitz was misspelled as “Bernard” and the surname of author B.J.A. Pollux was misspelled as “Pullox.” Finally, Dr. Keijer's first name was abbreviated rather than written in full. These errors, as well as various errors in the author affiliations, have now been corrected online

ANALISIS FAKTOR – FAKTOR YANG MEMPENGARUHI PERAN PRODUKTIF WANITA RUMAH TANGGA NELAYAN DI KOTA BENGKULU

The aims of this study were (1) to analyze the decision making pattern towards productive role aspects of the fisheries households women in Bengkulu City (2) to analyze the push factors that affect the productive role decision making of fisheries households women in Bengkulu City (3) to analyze the pull factors that affect the productive role decision making of fisheries households women in Bengkulu City (4) to analyze the pattern of productive role decision making of fisheries households women in Bengkulu City, based on the push and pull factors. There are 100 fisheries households women in Bengkulu City were selected with a simple random sampling method. Techniques of data analysis used qualitative descriptive and Multinomial Logit Regression. The result showed that productive role decision making mostly decided by women herself (wife only), with the percentages of encouragement to do the productive role aspect (40%), type of job aspect (54%), work place aspect (59%), and work time aspect (60%). According to every aspects, women/wife also dominated (39%) of productive role decision making. Push factors that have significant effect to the productive role decision making are education (EDU) and working experience (WORK) factors. Pull factor that has significant effect to the productive role decision making is family support (D 1 ) factor. The analysis of push and pull factors acknowledged that women have higher domination, t han men i n wom en’ s pro ducti ve ro le de cision maki ng Keywords: Productive Role, Decision Making, Fisheries Households Women, Multinomial Logit Regressio

Bi-exponential fitting and pharmacological blockade of AHP responses in limbic pyramidal neurons.

<p><i>A</i>: AHP recording from a representative CA1 neuron (gray) and the fit (dark gray) overlaid with the extracted decay of the medium (black-dotted) and sAHP (black-continuous) recorded from the same neuron before and after Forskolin (50 µm, 15 min) application. Inset shows the current protocol used to evoke the AHPs. <i>B</i>: AHP recording from a representative layer 2/3 lateral OFC neuron before and after Forskolin treatment. <i>C</i>: The goodness of fit (R²) to the AHP response is averaged across CA1 neurons recorded from the vehicle (gray diamond) and corticosterone (black circles) groups. <i>D</i>: Goodness of fit to the AHP response from layer 2/3 lateral OFC neurons. <i>E</i>: Goodness of fit to the AHP response from layer 2/3 prelimbic neurons. <i>F</i>: Goodness of fit to the AHP response from layer 2/3 infralimbic neurons. Number within brackets in the legends indicates number of neurons.</p

The long lasting effects of corticosterone treatment on AHPs and action potential firing of layer 2/3 infralimbic pyramidal neurons.

<p><i>A</i>: <i>left</i>: a sample pyramidal neuron filled with fluorescent dye. Inset: zoomed image of the neuron (white arrow). Scale bar equals 20 microns. <i>Right</i>, locations of the recorded neurons on a pictorial coronal mouse brain section. <i>B</i>: representative AHP responses from vehicle and corticosterone groups overlaid with the fit. Light gray: raw trace, thick dark gray: fit, dotted line: mAHP decay, thin black line: sAHP decay. Inset shows the current protocol used to evoke the AHPs. <i>C</i>: traces of action potentials from neurons representative of the vehicle and corticosterone groups. Marking indicate the positions of spike amplitude, half-width and threshold measurements. <i>D</i>: mAHP peak amplitude averaged per group and per current step. <i>E</i>: averaged values of sAHP peak amplitude for each current injection step. <i>F</i>: accommodation index (% ratio of the interspike intervals of the last two spikes to the first two spikes) from vehicle and corticosterone groups. Number within brackets in the legends indicates number of neurons.</p

Active and passive properties of neurons within each of the limbic brain regions were not significantly different between treatment (vehicle and corticosterone) groups.

<p>Active and passive properties of neurons within each of the limbic brain regions were not significantly different between treatment (vehicle and corticosterone) groups.</p

Stress-induced alterations in large-scale functional networks of the rodent brain

Stress-related psychopathology is associated with altered functioning of large-scale brain networks. Animal research into chronic stress, one of the most prominent environmental risk factors for development of psychopathology, has revealed molecular and cellular mechanisms potentially contributing to human mental disease. However, so far, these studies have not addressed the system-level changes in extended brain networks, thought to critically contribute to mental disorders. We here tested the effects of chronic stress exposure (10. days immobilization) on the structural integrity and functional connectivity patterns in the brain, using high-resolution structural MRI, diffusion kurtosis imaging, and resting-state functional MRI, while confirming the expected changes in neuronal dendritic morphology using Golgi-staining. Stress effectiveness was confirmed by a significantly lower body weight and increased adrenal weight. In line with previous research, stressed animals displayed neuronal dendritic hypertrophy in the amygdala and hypotrophy in the hippocampal and medial prefrontal cortex. Using independent component analysis of resting-state fMRI data, we identified ten functional connectivity networks in the rodent brain. Chronic stress appeared to increase connectivity within the somatosensory, visual, and default mode networks. Moreover, chronic stress exposure was associated with an increased volume and diffusivity of the lateral ventricles, whereas no other volumetric changes were observed. This study shows that chronic stress exposure in rodents induces alterations in functional network connectivity strength which partly resemble those observed in stress-related psychopathology. Moreover, these functional consequences of stress seem to be more prominent than the effects on gross volumetric change, indicating their significance for future research