Amniotic membrane as a scaffold in wound healing and diabetic foot ulcer: an experimental technique and recommendations

Background: Human amniotic membrane has been used clinically in a variety of applications for over the past 100 years and produced a significant amount of data in multiple areas of medicine. Its clinical usage ranges from wound coverage for burn victims to healing of the conjunctiva after pterygium repair. The amniotic membrane natural properties provide an easy to use, safe option for various medical applications. There is need to develop a method for storage of amniotic membrane which can retain the biological properties and as well have long shelf life too.Methods: The experimental technique was standardized for cryopreservation of amniotic membrane. For this, amniotic membrane was obtained from mothers who had delivered through caesarean section with their consent.Results: The standardized protocol for cryopreservation of amniotic membrane was found to be safe and preserved amniotic membrane is expected to have long shelf life.  Conclusions: The advantages associated with amniotic membrane such as easily available, inexpensive, non-immunogenic and antimicrobial, anti-inflammatory properties make it a suitable graft to be used in wound healing and diabetic foot ulcers

Biological and synthetic scaffold: an extra cellular matrix for constructive tissue engineering

Worldwide many people suffering from tissue dysfunctions or damages need rapid transplantation. Tissue engineering has attracted attention as therapeutic modality aiming at repairing lost or damaged tissues. Critical step in tissue engineering is fabrication of three dimensional scaffolds which mimic the extracellular matrix of tissues and promote tissue regeneration process. Extensive research has been carried out to develop a compatible scaffold which mimic the anatomical site of injury and as well as accessing the stem cells and growth factors to home on the injured site. The present article provides an overview on different scaffold approaches and materials used to fabricate scaffolds, with their properties and associated advantages and disadvantages. In particular, the therapeutic potential of amniotic membrane and collagen scaffold has been extensively reviewed in here

L'Institut de France et les anciennes Académies, par Léon Aucoc.

Bonnassieux Pierre. L'Institut de France et les anciennes Académies, par Léon Aucoc.. In: Bibliothèque de l'école des chartes. 1889, tome 50. pp. 464-465