Inhibition of activated factors II, VII, IX, and X by synthetic organic compounds directed against the active-site seryl residue

A series of 53 organic chemicals belonging to the groups of organic phosphates, sulfonyl derivatives and carbamates were screened for their activity against the coagulation factors IIa (thrombin), VIIa, IXa and Xa. Relatively specific inhibitors for the factors IIa, VIIa and Xa were found

Single-fly genome assemblies fill major phylogenomic gaps across the Drosophilidae Tree of Life

Long-read sequencing is driving rapid progress in genome assembly : across all major groups of life, including species of the family Drosophilidae, a longtime model system for genetics, genomics, and evolution. We previously developed a cost-effective hybrid Oxford Nanopore (ONT) long-read and Illumina short-read sequencing approach and used it to assemble 101 drosophilid genomes from laboratory cultures, greatly increasing the number of genome assemblies for this taxonomic group. The next major challenge is to address the laboratory culture bias in taxon sampling by sequencing genomes of species that cannot easily be reared in the lab. Here, we build upon our previous methods to perform amplification-free ONT sequencing of single wild flies obtained either directly from the field or from ethanol-preserved specimens in museum collections, greatly improving the representation of lesser studied drosophilid taxa in whole-genome data. Using Illumina Novaseq X Plus and ONT P2 sequencers with R10.4.1 chemistry, we set a new benchmark for inexpensive hybrid genome assembly at US $150 per genome while assembling genomes from as little as 35 ng of genomic DNA from a single fly. We present 183 new genome assemblies for 179 species as a resource for drosophilid systematics, phylogenetics, and comparative genomics. Of these genomes, 62 are from pooled lab strains and 121 from single adult flies. Despite the sample limitations of working with small insects, most single-fly diploid assemblies are comparable in contiguity (>1 Mb contig N50), completeness (>98% complete dipteran BUSCOs), and accuracy (>QV40 genome-wide with ONT R10.4.1) to assemblies from inbred lines. We present a well-resolved multi-locus phylogeny for 360 drosophilid and 4 outgroup species encompassing all publicly available (as of August 2023) genomes for this group. Finally, we present a Progressive Cactus whole-genome, reference-free alignment built from a subset of 298 suitably high-quality drosophilid genomes. The new assemblies and alignment, along with updated laboratory protocols and computational pipelines, are released as an open resource and as a tool for studying evolution at the scale of an entire insect family

Convergence analysis of the defect-correction iteration for hyperbolic problems

Ruralni turizam je jedan od ključnih čimbenika u revitalizaciji i održivom razvoju ruralnih područja. Bez obzira na evidentnu potražnju za ovakvim oblikom turizma, ruralni turizam u svom razvoju nailazi na velike poteškoće, marketinške, menadžerske i ekonomske prirode. Zbog svojih prirodnih i tradicijskih vrijednosti te dobrog prometnog položaja, Ličko-senjska županija ima sve preduvjete za razvoj ruralnog turizma. Međutim, unatoč navedenim prednostima, ruralni turizam na području Županije nije dovoljno razvijen. U cilju utvrđivanja problema i prepreka u razvoju ruralnog turizma, potencijala istog te preporučivanja poticajnih mjera za razvitak ruralnog turizma Ličko-senjske županije, provedeno je istraživanje čiji su rezultati izneseni u ovom radu. U prvoj je fazi provedeno intervjuiranje 17 predstavnika gradova, općina, turističkih zajednica i turističkih agencija, a u drugoj fazi je anketnim ispitivanjem anketirano 20 poslovnih subjekata (OPG i obrti) koji pružaju ugostiteljske i turističke usluge. Istraživanjem je utvrđena nedovoljna umreženost turističkih organizacija, lokalnih samoupravnih zajednica, poljoprivrednih i turističkih gospodarstava, koja bi se morala uspostaviti u svrhu kvalitetnije i učinkovitije turističke promocije Ličko-senjske županije. Rad Klastera Lika Destination osnovanog prije dvije godine u mnogome doprinosi rješavanju ovog problema.Rural tourism is one of the key factors in the revitalization and sustainable development of rural areas. Regardless of the evident demand for this type of tourism, rural tourism in its development is facing great difficulties such as marketing, managerial and economic issues. Due to its natural and traditional value and good traffic position, Lika-Senj County has all the preconditions for the development of rural tourism. However, despite these advantages, rural tourism in the County is underdeveloped. In order to identify problems and obstacles in the development of rural tourism, its potential and to recommend incentive measures for the development of rural tourism in the County of Lika-Senj, a study was conducted and the results are presented in this paper. In the first phase, 17 representatives of cities, municipalities, tourist boards and tourist agencies were interviewed and in the second phase, 20 business entities (OPG and crafts) providing catering and tourist services were surveyed. The research identified insufficient networking of tourism organizations, local self-governing communities, agricultural and tourist farms, which should be established for the purpose of better and more efficient tourism promotion of the Lika-Senj County. The work of Lika Destination Cluster that was established two years ago contributes greatly to solving this problem

Discerning size effect strengthening in ultrafine-grained Mg thin films

cited By 14International audienceMicrotensile experiments have been performed to elucidate the mechanical response of ultrafine-grained Mg thin films. Strengths of 160 MPa and elongations up to 8% were measured. Post-deformation electron microscopy indicates a lack of intragranular dislocation confinement. While strength does increase with decreasing grain size, the size effect for hexagonal Mg is not as strong as that reported for face-centered cubic metals. Strength appears to be governed by a lack of dislocation pile-up as well as texture and Peierls effects. © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved

Application of a solution-adaptive multigrid method to the Euler equations

this paper the adaptive multigrid method as developed at CWI during the first phase of the BRITE/EURAM Area 5 project (contract no. AERO-0003C) , is applied to the Euler equations of gas dynamics. The discretisations on the various levels of refinement introduce a set of nonlinear algebraic equations. The method to solve the set of nonlinear algebraic equations is an application of the nonlinear multigrid scheme, called full approximation storage (FAS), possibly embedded in the full multigrid (FMG) algorithm or an iterative defect correction (IDeC) process. First, these algorithms are described for their use in the present context of locally refined grids. After that, a strategy to introduce local refinements is described. In the experiments to follow, this strategy is used in the grid-refinement cycles. Next, some aspects of the implementation of a local refinement, multigrid method in a computer code are discussed. Finally, a number of examples are presented. The problems chosen for the experiments give an example of the possible range of application, where this method may be used as a tool for analysis of fluid dynamics problems. The problems considered are