Enzymatic pretreatment in the extraction process of soybean to improve protein and isoflavone recovery and to favor aglycone formation

This research aimed to evaluate the pretreatment of soybean with the carbohydrase multi-enzyme complex “Viscozyme L”, during the extraction process; in order to improve the recovery of proteins and isoflavones in soybase, and reduce the loss of these compounds through the okara residue. Three concentrations of enzyme were studied at 50 °C, along with an experiment carried out without enzyme addition (control experiment). The results were also evaluated in relation to standard soybase processing. In comparison to the standard and control processes, the enzymatic pretreatment reduced up to 85% the total amount of okara residue. Due to the action of the multi-enzyme complex, protein and total isoflavone recovery increased from 42% to 83% and from 59% to 93%, respectively. The application of Viscozyme L also favored the conversion of conjugated forms of isoflavone to aglycone in the soybase, representing up to 50% of the total isoflavones. The enzymatic pretreatment of soybean with carbohydrase improved the nutritional quality of the soybase, while at the same time reducing residue generation; showing that the proposed food process can be considered environmentally friendly method137CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçãoSem informação2017/16835-

Rotating-pulsed fluidized bed drying of okara: evaluation of process kinetic and nutritive properties of dried product

Okara is a byproduct of soymilk processing and is rich in fiber and protein. It is underutilized in the food industry as an ingredient owing to its high perishability. The objective of this work was to study the drying of okara in a rotating-pulsed fluidized bed dryer to verify the effect of air temperature (50–90 °C) and frequency of disc rotation (7.5–24.5 Hz) on the inactivation of trypsin inhibitors and the retention of the total phenolic compounds and isoflavones. The drying process was effective in drying this cohesive material. The process conditions exhibited no significant effect on the activity of trypsin inhibitors and content of total isoflavones. However, the content of total phenolic compounds was affected by the air temperature, in which the higher retention occurred at 70 °C and 16 Hz. Regarding the conversion of isoflavone classes, the drying process favored the hydrolysis of malonylglycosides and β-glycosides into aglycones1316111620FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP17/16835-