Stability-indicating liquid chromatographic and UV spectrophotometric methods for the quantification of ciprofibrate in capsules and tablets

This study describes the development and evaluation of stability-indicating liquid chromatographic (LC) and UV spectrophotometric methods for the quantification of ciprofibrate (CPF) in tablets and capsules. Isocratic LC separation was achieved on a RP18 column using a mobile phase of o-phosphoric acid (0.1% v/v), adjusted to pH 3.0 with triethylamine (10% v/v) and acetonitrile (35:65 v/v), with a flow rate of 1.0 mL min-1. Detection was achieved with a photodiode array detector at 233 nm. For the spectrophotometric analysis, ethanol and water were used as the solvent and a wavelength of 233 nm was selected for the detection. The methods were validated according to International Conference on Harmonization (ICH) guidelines for validating analytical procedures. Statistical analysis showed no significant difference between the results obtained by the two methods. The proposed methods were successfully applied to the CPF quality-control analysis of tablets and capsules.Este estudo descreve o desenvolvimento e avaliação de método indicativo da estabilidade por cromatografia líquida (LC) e método por espectrofotometria UV para quantificação de ciprofibrato (CPF) em comprimidos e cápsulas. No método por cromatografia líquida as análises foram realizadas isocraticamente em coluna de fase reversa C18, utilizando fase móvel composta por ácido o-fosfórico (0.1% v/v) pH 3.0, ajustado com trietilamina (10% v/v), e acetonitrila (35:65 v/v), com fluxo de 1,0 mL min-1. A detecção foi realizada em detector de arranjo de diodos a 233 nm. Na análise espectrofotométrica, etanol e água foram utilizados como solventes e o comprimento de onda de 233 nm foi selecionado para a detecção do fármaco. Os métodos foram validados de acordo com as diretrizes do International Conference on Harmonization (ICH). A análise estatística não mostrou diferença significativa entre os resultados obtidos pelos dois métodos. Os métodos foram aplicados com sucesso para análises de controle de qualidade do ciprofibrato em comprimidos e cápsulas

Ambiente Virtual de Ensino em Laboratórios de Química (AQuí): Expandindo o Ensino no YouTube:

Digital information and communication technology tools have been used as allies in teaching. Chemistry teaching sometimes requires practical activities in laboratories to elucidate the content better, and using videos aimed at this approach can help in the teaching-learning process. This descriptive, qualitative and quantitative study aims to describe the production of manual videos to handle equipment in chemistry laboratories and analyze the scope of the material produced and made available on the "Projeto AQuí" channel on YouTube. Undergraduate students' teaching material was prepared, following the pre-production, production, and post-production steps described in this work. Through the indicators obtained by Google Analytics, it was possible to verify the impact of the material produced. Through the comments left voluntarily on the page, it was possible to perceive viewers' satisfaction with the material. Based on the metrics analyzed, the "Projeto AQuí" channel on YouTube fulfills its proposal to disseminate material on laboratory operations, with a national and international reach.   Keywords: DICT. Technology in education.Video. Laboratory equipment.As ferramentas de tecnologia digitais da informação e comunicação vem sendo utilizadas como aliadas no ensino. O ensino de química por vezes necessita de atividades práticas em laboratórios para melhor elucidar o conteúdo, e utilizar vídeos voltados para esta abordagem podem auxiliar no processo de ensino aprendizagem. Este estudo, de caráter descritivo, qualitativo e quantitativo, tem por objetivo descrever a confecção de vídeos-manuais voltados ao manuseio de equipamentos em laboratórios de química, e analisar o alcance e a satisfação do material produzido e disponibilizado no canal “Projeto AQuí” no YouTube. O material didático foi elaborado por alunos de graduação, seguindo os passos de pré-produção, produção e pós produção descritos neste trabalho. Através dos indicadores obtidos pelo YouTube Analytics, foi possível verificar o alcance do material produzido, e através dos comentários deixados voluntariamente na página foi possível perceber a satisfação dos espectadores com o material. Com base nas métricas analisadas, o canal “Projeto AQuí” no YouTube cumpre sua proposta de divulgação de material sobre operações laboratoriais, com um alcance nacional e internacional, sendo utilizado por instituições de ensino técnico e de ensino superior.   Palavras-chave: TDIC. Material didático. Tecnologia na educação. Vídeo. Equipamentos de laboratório

Stability-indicating liquid chromatographic and UV spectrophotometric methods for the quantification of ciprofibrate in capsules and tablets

This study describes the development and evaluation of stability-indicating liquid chromatographic (LC) and UV spectrophotometric methods for the quantification of ciprofibrate (CPF) in tablets and capsules. Isocratic LC separation was achieved on a RP18 column using a mobile phase of o-phosphoric acid (0.1% v/v), adjusted to pH 3.0 with triethylamine (10% v/v) and acetonitrile (35:65 v/v), with a flow rate of 1.0 mL min-1. Detection was achieved with a photodiode array detector at 233 nm. For the spectrophotometric analysis, ethanol and water were used as the solvent and a wavelength of 233 nm was selected for the detection. The methods were validated according to International Conference on Harmonization (ICH) guidelines for validating analytical procedures. Statistical analysis showed no significant difference between the results obtained by the two methods. The proposed methods were successfully applied to the CPF quality-control analysis of tablets and capsules

DESENVOLVIMENTO E VALIDAÇÃO DE MÉTODO POR CLAE-DAD E ESTUDO PRELIMINAR DA ESTABILIDADE DA SINEFRINA EM EXTRATO SECO DE Citrus aurantium L

A reversed-phase liquid chromatographic method was developed for the assay of synephrine in Citrus aurantium dry extract. The chromatographic separation used a C18 (250 × 4.6 mm, 5 µm) column and a mobile phase consisting of acetonitrile: 0.01% orthophosphoric acid (pH 3.0) at a 15:85 ratio, flow rate of 1 mL min-1 and detection at 220 nm. The detector response was linear in the range 15 - 45 mg mL-1. Specificity and stability, indicating suitability of the method, were confirmed using stress conditions (acid and basic hydrolysis, oxidation and photolysis). Intraday precision yielded RSD values of less than 1.77% and interday precision of 2.5%. The average recovery accuracy of the test was 102.03%. The method was validated according to international guidelines and can be used in routine quality control and stability studies of Citrus aurantium preparations. The preliminary stability study showed that synephrine is susceptible to hydrolysis, oxidation and photolysis