44 research outputs found

    Preprocessing by <i>Wa</i>.

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    <p>Rows represent the three classes fusiform, epithelioid and cobblestone. <i>Left</i>: original image; <i>right</i>: horizontal, vertical and diagonal details.</p

    Preprocessing by <i>OR</i>.

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    <p>Rows represent the three classes fusiform, epithelioid and cobblestone. <i>Left</i>: original image; <i>right</i>: the three oriented images.</p

    Collagen-immobilized polyimide membranes for retinal pigment epithelial cell adherence and proliferation

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    Degenerative retinal diseases are a leading cause of visual loss and irreversible blindness, particularly in the developed world. Retinal pigment cell (RPE) transplantation is nowadays considered the most promising therapeutic approach for certain retinal diseases, and the presence of a supportive scaffold has been considered essential to ensure the success of the implant. In this work, collagen IV was covalently immobilized to the surface of polyimide membranes, with the purpose of developing scaffold materials for RPE cell culture. The covalent modification method involved four steps: argon-plasma treatment, acrylic acid graft polymerization, surface activation, and finally immobilization of collagen type IV. Collagen-modified membranes did not become more rough but became significantly more hydrophilic than the unmodified and dip-coated controls. ARPE-19 cell morphology and attachment were studied by immunofluorescence staining and confocal microscopy. Covalently modified surfaces showed cell attachment and cell properties comparable to the uncoated and dip-coated controls. This work demonstrated the potential of collagen IV-immobilized polyimide membranes as substrates for the growth of ARPE-19 cells.publishedVersionPeer reviewe

    Fittings of the model to the experimental data.

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    <p>(A) GA-treated, and (B) control data sets with dashed lines representing the data in dimensionless NF units and solid lines representing the model simulations with arbitrary units representing [Ca<sup><b>2+</b></sup>]<sub>i</sub> in μM concentrations. The uppermost curve pair (blue) represents NB1, the second uppermost NB2 (green), followed by NB3 (red), NB4 (light blue), NB5 (purple), NB6 (yellow), NB7 (black), NB8 (light red), NB9 (grey), and NB10 (orange).</p

    Preprocessing by Gabor filters.

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    <p>Rows represent the three classes fusiform, epithelioid and cobblestone. <i>Left</i>: original image; <i>right</i>: convolved images at scale 4.</p
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