36 research outputs found
An EU Perspective on Biosafety Considerations for Plants Developed by Genome Editing and Other New Genetic Modification Techniques (nGMs)
The question whether new genetic modification techniques (nGM) in plant development might result in non-negligible negative effects for the environment and/or health is significant for the discussion concerning their regulation. However, current knowledge to address this issue is limited for most nGMs, particularly for recently developed nGMs, like genome editing, and their newly emerging variations, e.g., base editing. This leads to uncertainties regarding the risk/safety-status of plants which are developed with a broad range of different nGMs, especially genome editing, and other nGMs such as cisgenesis, transgrafting, haploid induction or reverse breeding. A literature survey was conducted to identify plants developed by nGMs which are relevant for future agricultural use. Such nGM plants were analyzed for hazards associated either (i) with their developed traits and their use or (ii) with unintended changes resulting from the nGMs or other methods applied during breeding. Several traits are likely to become particularly relevant in the future for nGM plants, namely herbicide resistance (HR), resistance to different plant pathogens as well as modified composition, morphology, fitness (e.g., increased resistance to cold/frost, drought, or salinity) or modified reproductive characteristics. Some traits such as resistance to certain herbicides are already known from existing GM crops and their previous assessments identified issues of concern and/or risks, such as the development of herbicide resistant weeds. Other traits in nGM plants are novel; meaning they are not present in agricultural plants currently cultivated with a history of safe use, and their underlying physiological mechanisms are not yet sufficiently elucidated. Characteristics of some genome editing applications, e.g., the small extent of genomic sequence change and their higher targeting efficiency, i.e., precision, cannot be considered an indication of safety per se, especially in relation to novel traits created by such modifications. All nGMs considered here can result in unintended changes of different types and frequencies. However, the rapid development of nGM plants can compromise the detection and elimination of unintended effects. Thus, a case-specific premarket risk assessment should be conducted for nGM plants, including an appropriate molecular characterization to identify unintended changes and/or confirm the absence of unwanted transgenic sequences
Corrigendum: An EU Perspective on Biosafety Considerations for Plants Developed by Genome Editing and Other New Genetic Modification Techniques (nGMs)
The question whether new genetic modification techniques (nGM) in plant development might result in non-negligible negative effects for the environment and/or health is significant for the discussion concerning their regulation. However, current knowledge to address this issue is limited for most nGMs, particularly for recently developed nGMs, like genome editing, and their newly emerging variations, e.g., base editing. This leads to uncertainties regarding the risk/safety-status of plants which are developed with a broad range of different nGMs, especially genome editing, and other nGMs such as cisgenesis, transgrafting, haploid induction or reverse breeding. A literature survey was conducted to identify plants developed by nGMs which are relevant for future agricultural use. Such nGM plants were analyzed for hazards associated either (i) with their developed traits and their use or (ii) with unintended changes resulting from the nGMs or other methods applied during breeding. Several traits are likely to become particularly relevant in the future for nGM plants, namely herbicide resistance (HR), resistance to different plant pathogens as well as modified composition, morphology, fitness (e.g., increased resistance to cold/frost, drought, or salinity) or modified reproductive characteristics. Some traits such as resistance to certain herbicides are already known from existing GM crops and their previous assessments identified issues of concern and/or risks, such as the development of herbicide resistant weeds. Other traits in nGM plants are novel; meaning they are not present in agricultural plants currently cultivated with a history of safe use, and their underlying physiological mechanisms are not yet sufficiently elucidated. Characteristics of some genome editing applications, e.g., the small extent of genomic sequence change and their higher targeting efficiency, i.e., precision, cannot be considered an indication of safety per se, especially in relation to novel traits created by such modifications. All nGMs considered here can result in unintended changes of different types and frequencies. However, the rapid development of nGM plants can compromise the detection and elimination of unintended effects. Thus, a case-specific premarket risk assessment should be conducted for nGM plants, including an appropriate molecular characterization to identify unintended changes and/or confirm the absence of unwanted transgenic sequences
Climate Change and the Potential Spreading of Marine Mucilage and Microbial Pathogens in the Mediterranean Sea
Background: Marine snow (small amorphous aggregates with colloidal properties) is present in all oceans of the world.
Surface water warming and the consequent increase of water column stability can favour the coalescence of marine snow
into marine mucilage, large marine aggregates representing an ephemeral and extreme habitat. Marine mucilage
characterize aquatic systems with altered environmental conditions.
Methodology/Principal Findings: We investigated, by means of molecular techniques, viruses and prokaryotes within the
mucilage and in surrounding seawater to examine the potential of mucilage to host new microbial diversity and/or spread
marine diseases. We found that marine mucilage contained a large and unexpectedly exclusive microbial biodiversity and
hosted pathogenic species that were absent in surrounding seawater. We also investigated the relationship between
climate change and the frequency of mucilage in the Mediterranean Sea over the last 200 years and found that the number
of mucilage outbreaks increased almost exponentially in the last 20 years. The increasing frequency of mucilage outbreaks is
closely associated with the temperature anomalies.
Conclusions/Significance: We conclude that the spreading of mucilage in the Mediterranean Sea is linked to climate-driven
sea surface warming. The mucilage can act as a controlling factor of microbial diversity across wide oceanic regions and
could have the potential to act as a carrier of specific microorganisms, thereby increasing the spread of pathogenic bacteria
Activism and radical politics in the digital age: Towards a typology
This article aims to develop a typology for evaluating different types of activism in the digital age, based on the ideal of radical democracy. Departing from this ideal, activism is approached in terms of processes of identification by establishing conflictual frontiers to outside Others as either adversaries or enemies. On the basis of these discussions, we outline a typology of four kinds of activists: the salon activist, the contentious activist, the law-abiding activist, and the Gandhian activist. The typologyâs first axis, between antagonism and agonism, is derived from normative discussions in radical democracy concerning developing frontiers. The second axis, about readiness to engage in civil disobedience, is derived from a review of studies of different forms of online activism. The article concludes by suggesting that the different forms of political engagement online have to be taken into account when studying how online activism can contribute to social change
Secondary mineral formation associated with respiration of nontronite, NAu-1 by iron reducing bacteria
Experimental batch and miscible-flow cultures were studied in order to determine the mechanistic pathways of microbial Fe(III) respiration in ferruginous smectite clay, NAu-1. The primary purpose was to resolve if alteration of smectite and release of Fe precedes microbial respiration. Alteration of NAu-1, represented by the morphological and mineralogical changes, occurred regardless of the extent of microbial Fe(III) reduction in all of our experimental systems, including those that contained heat-killed bacteria and those in which O(2), rather than Fe(III), was the primary terminal electron acceptor. The solid alteration products observed under transmission electron microscopy included poorly crystalline smectite with diffuse electron diffraction signals, discrete grains of Fe-free amorphous aluminosilicate with increased Al/Si ratio, Fe-rich grains, and amorphous Si globules in the immediate vicinity of bacterial cells and extracellular polymeric substances. In reducing systems, Fe was also found as siderite. The small amount of Fe partitioned to the aqueous phase was primarily in the form of dissolved Fe(III) species even in the systems in which Fe(III) was the primary terminal electron acceptor for microbial respiration. From these observations, we conclude that microbial respiration of Fe(III) in our laboratory systems proceeded through the following: (1) alteration of NAu-1 and concurrent release of Fe(III) from the octahedral sheets of NAu-1; and (2) subsequent microbial respiration of Fe(III)
Optimal Preparation of Water Samples for the Examination of Colloidal Material by Transmission Electron Microscopy.
EU workshop: Monitoring of environmental impacts of genetically modified plants
The main goal of the workshop was to initiate a discussion on how monitoring of GMOs should be - or can be - implemented. Monitoring shall help to detect in an early stage possible 'direct', 'indirect', 'immediate' and 'delayed' effects resulting from the deliberate release of GMOs into the environment, but there is still no consensus on the scope, the methods and the duration of such monitoring programmes. Since the term 'monitoring', but also other terms in this field (e.g. general surveillance) are still subject to various interpretations, it was also necessary to deal with definitions. Moreover the intention of the workshop was an exchange of experience on monitoring projects already conducted in some of the Member States and an exchange of views on monitoring concepts developed so far. (orig.)Ziel des Workshops war es, eine Diskussion darueber zu beginnen, wie das Monitoring von GVO umgesetzt werden kann bzw. soll. Ein Monitoring soll dazu dienen, so frueh wie moeglich moegliche direkte, indirekte, unmittelbare und verzoegerte Effekte, die sich aus der Freisetzung von GVO in die Umwelt ergeben koennten, zu erfassen. Ueber den Umfang, die Methoden und die Dauer solcher Monitoringprogramme besteht allerdings noch weitgehend Uneinigkeit. Da der Begriff 'Monitoring' - aber auch andere Begriffe in diesem Zusammenhang, wie zum Beispiel 'general surveillance' - unterschiedlich interpretiert werden, war es auch notwendig, sich mit der Bestimmung dieser und anderer Begriffe zu beschaeftigen. Darueber hinaus war beabsichtigt, einen Ueberblick ueber Monitoringprojekte, die in einzelnen Mitgliedstaaten existieren und einen Meinungsaustausch ueber bereits entwickelte Monitoringkonzepte zu ermoeglichen. (orig.)SIGLEAvailable from TIB Hannover: RN 8422(2001,45) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Umwelt, Naturschutz und Reaktorsicherheit, Bonn (Germany); Commission of the European Communities, Brussels (Belgium). DG EnvironmentDEGerman
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Available from TIB Hannover: RN 8422(2003,68) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Umwelt, Naturschutz und Reaktorsicherheit, Bonn (Germany)DEGerman