14 research outputs found
Development of a continuous aqueous two-phase flotation process for the downstream processing of biotechnological products
Impedance-Based Performance Analysis of Micropatterned Polymer Electrolyte Membrane Fuel Cells
Twist exome capture allows for lower average sequence coverage in clinical exome sequencing
Background Exome and genome sequencing are the predominant techniques in the diagnosis and research of genetic disorders. Sufficient, uniform and reproducible/consistent sequence coverage is a main determinant for the sensitivity to detect single-nucleotide (SNVs) and copy number variants (CNVs). Here we compared the ability to obtain comprehensive exome coverage for recent exome capture kits and genome sequencing techniques. Results We compared three different widely used enrichment kits (Agilent SureSelect Human All Exon V5, Agilent SureSelect Human All Exon V7 and Twist Bioscience) as well as short-read and long-read WGS. We show that the Twist exome capture significantly improves complete coverage and coverage uniformity across coding regions compared to other exome capture kits. Twist performance is comparable to that of both short- and long-read whole genome sequencing. Additionally, we show that even at a reduced average coverage of 70× there is only minimal loss in sensitivity for SNV and CNV detection. Conclusion We conclude that exome sequencing with Twist represents a significant improvement and could be performed at lower sequence coverage compared to other exome capture techniques
Analyse und Modellbildung von PEM-Brennstoffzellen mittels elektrochemischer Impedanzspektroskopie
Herstellung multifunktionaler Profile und Profilverbindungen durch umformende und spanende Prozesse
Das Teilprojekt B4 des Sonderforschungsbereichs 666 beschäftigt sich mit der Herstellung von verzweigten Blechprofilen mittels umformenden und spanenden Fertigungsverfahren. Im folgenden Beitrag werden Erweiterungen des Fertigungsprozesses hinsichtlich neuartiger Profilverbindungen und Mul-tifunktionalität von Spaltprofilen aufgezeigt. Im Bereich der Umformtechnik werden Herausforderun-gen bei der Umformung der hochverfestigten Spalt-profilflansche sowie ein prozessintegriertes Fügen von zusätzlichen Elementen vorgestellt. In der spa-nenden Bearbeitung wird ein Werkstück-Führungssystem für die Bearbeitung von Flansch- und Bandkanten sowie ein mitfahrendes Spannsys-tem für die Herstellung von diskontinuierlichen Formelementen und Funktionsflächen an nicht-linearen Spaltprofilen vorgestellt. Zusätzlich wird auf eine Möglichkeit zur Verbesserung der Bearbei-tungsqualität bei der spanenden Bearbeitung von Blechprofilen durch eine aktive Prozessstabilisierung eingegange
Extracellular Cyclophilin A Augments Platelet-Dependent Thrombosis and Thromboinflammation
Impedance modelling of porous electrode structures in polymer electrolyte membrane fuel cells
ACKR3 regulates platelet activation and ischemia-reperfusion tissue injury
Platelet activation plays a critical role in thrombosis. Inhibition of platelet activation is a cornerstone in treatment of acute organ ischemia. Platelet ACKR3 surface expression is independently associated with all-cause mortality in CAD patients. In a novel genetic mouse strain, we show that megakaryocyte/platelet-specific deletion of ACKR3 results in enhanced platelet activation and thrombosis in vitro and in vivo. Further, we performed ischemia/reperfusion experiments (transient LAD-ligation and tMCAO) in mice to assess the impact of genetic ACKR3 deficiency in platelets on tissue injury in ischemic myocardium and brain. Loss of platelet ACKR3 enhances tissue injury in ischemic myocardium and brain and aggravates tissue inflammation. Activation of platelet-ACKR3 via specific ACKR3 agonists inhibits platelet activation and thrombus formation and attenuates tissue injury in ischemic myocardium and brain. Here we demonstrate that ACKR3 is a critical regulator of platelet activation, thrombus formation and organ injury following ischemia/reperfusion