Assessment of babA2 and hsp genotype frequency in Helicobacter pylori specimens isolated from digestive disorders patients

Background and Objective: Helicobacter pylori is the main gastric pathogen in human. BabA2 and Hsp genotypes are essential for enormous clinical outcomes in gastreodeoneal and dyspepsia. This study was done to determine the assessment of babA2 and hsp genotype frequency in Helicobacter pylori specimens isolated from digestive disorders patients. Method: This descriptive-analytic study was carried out on 80 digestive disorders patients in 5 th hospital, Gorgan, northern Iran. Stomach specimen biopsy was taken by a gastroenterologist. Urease test, histopathologic assessment and DNA extraction were performed. The frequency of babA2 and hsp genotypes was determined using poly merase chain reaction. Results: In 80 affected patients with H.pylori, 36, 18 and 26 patients were found to suffer from gastritis, stomach cancer and stomach ulcer, respectively. 51 specimens (63%) were positive babA2 genotype. 49 specimens (61%) were positive hsp genotype. No significant relationship was found between babA2 and hsp geno types with stomach diseases. Conclusion: In spite of positive babA2 and hsp genotype in isolated Helicobacter pylori speicments from digestive disorders patients, this finding was not correlated with type of digestive disorders

Transcriptional regulation of the human ALDH1A1 promoter by the oncogenic homeoprotein TLX1/HOX11

The homeoprotein TLX1, which is essential to spleen organogenesis and oncogenic when aberrantly expressed in immature T cells, functions as a bifunctional transcriptional regulator, being capable of activation or repression depending on cell type and/or promoter context. However, the detailed mechanisms by which it regulates the transcription of target genes such as ALDH1A1 remains to be elucidated. We therefore functionally assessed the ability of TLX1 to regulate ALDH1A1 expression in two hematopoietic cell lines, PER-117 T-leukemic cells and human erythroleukemic (HEL) cells, by use of luciferase reporter and mobility shift assays. We showed that TLX1 physically interacts with the general transcription factor TFIIB via its homeodomain, and identified two activities in respect to TLX1-mediated regulation of the CCAAT box-containing ALDH1A1 promoter. The first involved CCAAT-dependent transcriptional repression via perturbation of GATA factor-containing protein complexes assembled at a non-canonical TATA (GATA) box. A structurally intact homeodomain was essential for repression by TLX1 although direct DNA binding was not required. The second activity, which involved CCAAT-independent transcriptional activation did not require an intact homeodomain, indicating that the activation and repression functions of TLX1 are distinct. These findings confirm ALDH1A1 gene regulation by TLX1 and support an indirect model for TLX1 function, in which protein-protein interactions, rather than DNA binding at specific sites, are crucial for its transcriptional activity

Intra-Abdominal Pressure Monitoring By Nurses For Early Detection Of Abdominal Compartment Syndrome And Early Decompressed Laparotomy In Comparison With Need To Laparotomy By Physical Exam.

Abstract:Background& Objective:Abdominal compartment syndrome is as potentially lethal condition caused by any event that produces intra-abdominal hypertension. The most common cause is blunt abdominal trauma. Increasing intra-abdominal pressure affects much vital body system. Hemodynamic, respiratory, renal and neurological abnormalities are hallmark of ACS. All authors are agreeing that decompression laparotomy is the treatment of choice for the ACS. Nursing care involves vigilant monitoring for early detection including serial measurement of intra-abdominal pressure (from urinary bladder). This study was designed to investigate the comparative effect of measurement of intra-abdominal pressure and physical exam in patient with ACS for refer the patients to operation room. Methodology:In this research ACS was defined as abdominal distention with intra-abdominal pressure ≥15 mm Hg (20cm H2O) accompanied by at least two of the following: oliguria or anuria, hypoxemia and hypotension or shock. When ACS detected by researcher, compared with diagnose of need to laparotomy by physician both timely and numerically.Results:Of 100 patients admitted over 8 months, 28 patients had abdominal compartment syndrome, 21 of this patients refer to operation room by physician. According to numeral comparison between two group not significant (P>.05) but according the time measuring abdominal pressure for detection of need to decompressed laparotomy significantly decreased than physical exam (p<.05).Conclusion:The ACS is a potentially lethal condition occurs in a significant number of severely blunt abdominal trauma patients and it develops quickly. Preventative therapy should be instituted to minimize its development in patients at risk and monitoring of intraabdominal pressure may allow prompt treatment of this condition rather than physical exam

COMPARISON OF SECOND-DEGREE BURNS HEALING TIME IN TWO METHODS OF DRESSING WITH FUNDERMOL HERBAL OINTMENT AND% 1 SILVER SULFADIAZINE OINTMENT‏

BACKGROUND: Burn wounds are one of the health problems in modern societies that are associated with irreparable harms and many side problems for patients and their families. Infection due to burn wounds is the main cause of death in such patients. One of the methods to prevent infection of burn wounds is topical antibiotic ointments. This study aimed to investigate and identify effective ointments to treat burn wounds. For this purpose, the effects of two types of ointment, fundermol and 1% silver sulfadiazine cream on second degree burn wounds were compared. METHODS: This was a clinical trial study conducted in 2008. Using convenient and continuous sampling method, 50 patients referred to Imam Mousa Kazem Burn Injury Clinic in Isfahan, Iran with 2nd degree burn wounds in 1% to 10% surface area were enrolled. The patients were randomly divided into two groups of treatment with fundermol and sulfadiazine and the dressing was changed once a day. The healing time for burn wounds in each patient was recorded in a checklist and data were analyzed by independent t-test via SPSS software. RESULTS: The healing time of burn wounds in the group treated with fundermol was shorter than that in the group treated with sulfadiazine (p < 0.001). CONCLUSIONS: The present study showed that fundermol ointment accelerates burn wound healing. Therefore, fundermol can be introduced as a good replacement for current treatments of burn wounds

Comparison of Intra-abdominal Pressure Measurement and Physical Exam for Diagnosis of Surgery Indication in Patients with Abdominal Compartment Syndrome due to Blunt Trauma

Background & Aims: Increase in abdominal pressure can lead to the so-called intra–abdominal compartment syndrome (ACS) that is often observed during the first 24 hours after sever abdominal trauma and surgery. Measurement of the intra abdominal pressure through the bladder as a non-invasive measurement can provide a quick and accurate assessment of abdominal pressure changes. This study was performed to compare the diagnostic value of intra-abdominal pressure measurement through the bladder with that of physical exam in the diagnosis of surgery indication. Methods: This descriptive- analytical study was performed on patients with intra-abdominal compartment syndrome due to blunt abdominal trauma referred to Nemazee hospital, Shiraz, Iran. Tools for data collection included a check list consisting demographic information, and intra-abdominal pressure measurement instruments. Data analysis was done through SPSS software. Results: Of 100 patients with abdominal trauma whose abdominal pressures were measured, 28 ones had abdominal compartment syndrome of whom, 21 ones (75 percent) were referred to the operation room by physician. Among all patients who were sent to the surgery room, 5 patients (23.80%) were survived and all those who were not sent to the surgery room died. Mean diagnosis time of measuring abdominal pressure for detection of operation indication was significantly lower than that of physical exam (P<0.01). Age, sex, type of trauma and type of injury to internal organ had no significant relationship with the rate of abdominal compartment syndrome. Also, there was no significant difference between the two methods in finding surgery indication. Conclusion: Implementing education on methods of early diagnosis of intra-abdominal pressure increase for medical team especially nurses is one of the treatment prioritie

Association of Milk and Dairy Products Consumption During Pregnancy with Fetal and Neonatal Head Circumferences: A Systematic Review

Context: Milk and dairy products consumed by mothers seem to be effective for fetal and neonatal anthropometric measurements, because they contain various nutrients. Objectives: The aim of this study was to systematically review the influence of milk and dairy products consumption by mothers on fetal and neonatal head circumferences. Data Sources: Systematic searches were conducted in electronic databases including PubMed/MEDLINE, Scopus, ISI, Ovid, Embase, Medlib, Google Scholar, clinical trials and Cochrane central register of clinical trials. Study Selection: All studies that assessed the relationship between milk and dairy products consumption in healthy females during pregnancy and fetal and neonatal head circumferences were included in our systematic review. Finally, seven studies were relevant that included five cohort studies, one cross-sectional study and one randomized clinical trial. Data Extraction: This systematic review was performed based on the preferred reporting item for systematic reviews and meta-analysis (PRISMA) statement recommendation, and for quality assessment, the Newcastle-Ottawa scale (NOS) for cohort studies, the adapted NOS for a cross-sectional study and the Jadad quality assessment score for a randomized clinical trial, were used. Results: Seven studies that comprised of more than 50000 pregnant females were included in this review. One cohort study, one cross-sectional study and one randomized controlled trial study showed that milk or dairy products consumption by pregnant mothers was not associated with neonatal birth head circumference, while three cohort studies reported that maternal milk or dairy products intake had a positive effect on neonatal birth head circumference. Two cohort studies showed that there was no relationship between maternal milk or dairy products consumption and fetal head circumference while a cross-sectional study reported that there was a positive relationship between milk or dairy products consumption during pregnancy and fetal head circumference. Conclusions: Evidences in this field are limited and inconsistent. According to the findings, there is a positive association between milk and dairy products consumption by pregnant mothers and neonatal birth head circumference but there is no relationship between maternal milk and dairy products consumption and fetal head circumference. However, almost all studies emphasized the importance of milk and dairy products in the maternal diet as a source of valuable nutrients

Fluctuations, Finite-Size Effects and the Thermodynamic Limit in Computer Simulations: Revisiting the Spatial Block Analysis Method

The spatial block analysis (SBA) method has been introduced to efficiently extrapolate thermodynamic quantities from finite-size computer simulations of a large variety of physical systems. In the particular case of simple liquids and liquid mixtures, by subdividing the simulation box into blocks of increasing size and calculating volume-dependent fluctuations of the number of particles, it is possible to extrapolate the bulk isothermal compressibility and Kirkwood–Buff integrals in the thermodynamic limit. Only by explicitly including finite-size effects, ubiquitous in computer simulations, into the SBA method, the extrapolation to the thermodynamic limit can be achieved. In this review, we discuss two of these finite-size effects in the context of the SBA method due to (i) the statistical ensemble and (ii) the finite integration domains used in computer simulations. To illustrate the method, we consider prototypical liquids and liquid mixtures described by truncated and shifted Lennard–Jones (TSLJ) potentials. Furthermore, we show some of the most recent developments of the SBA method, in particular its use to calculate chemical potentials of liquids in a wide range of density/concentration conditions

The quality of life in boys with Duchenne muscular dystrophy

We conducted a study to evaluate the quality of life in boys with Duchenne muscular dystrophy aged 8–18 years, compared with that in matched healthy controls. A total of 85 boys with Duchenne muscular dystrophy aged 8–18 years and 136 age, sex and living place matched healthy controls were included in this study. Patients and one of their parents separately completed the 27-item Persian version of KIDSCREEN questionnaire (child and adolescent version and parent version). From the children's perspective, the quality of life in patients was found to be lower in two subclasses: “physical activities and health” (p < 0.001) and “friends” (p = 0.005). Parental estimation of their sick child's quality of life was significantly lower than children's own assessment in two subclasses: “physical activities and health” (p < 0.001) and “general mood and feelings” (p < 0.001). Our results indicate that boys with Duchenne muscular dystrophy have quite a satisfactory quality of life. A happier and more hopeful life can be promoted through increasing social support and improving the parental knowledge regarding their child's more positive life perspective. © 2016 Elsevier B.V

Thermo-Mechanical Buckling and Non-Linear Free Oscillation of Functionally Graded Fiber-Reinforced Composite Laminated (FG-FRCL) Beams

We investigated the thermal buckling temperature and nonlinear free vibration of functionally graded fiber-reinforced composite laminated (FG-FRCL) beams. The governing nonlinear partial differential equations were derived from the Euler-Bernoulli beam theory, accounting for the von Karman geometrical nonlinearity. Such equations were then reduced to a single equation by neglecting the axial inertia. Thus, the Galerkin method was applied to discretize the governing nonlinear partial differential equation in the form of a nonlinear ordinary differential equation, which was then solved analytically according to the He's variational method. Three different boundary conditions were selected, namely simply, clamped and clamped-free supports. We also investigated the effect of power-index, lay-ups, and uniform temperature rise on the nonlinear natural frequency, phase trajectory and thermal buckling of FG-FRCL beams. The results showed that FG-FRCL beams featured the highest fundamental frequency, whereas composite laminated beams were characterized by the lowest fundamental frequency. Such nonlinear frequencies increase for an increased power index and a decreased temperature. Finally, it was found that FG-FRCL beams with [0/0/0] lay-ups featured the highest nonlinear natural frequency and the highest thermal buckling temperature, followed by [0/90/0] and [90/0/90] lay-ups, while a [90/90/90] lay-up featured the lowest nonlinear natural frequency and critical buckling temperature