Seroprevalence and “Knowledge, Attitudes and Practices” (KAPs) survey of endemic ovine brucellosis in Egypt

Additional file 1. Questionnaire used to collect shepherds’ knowledge, attitudes and practices on ovine brucellosis

A multifaceted risk model of brucellosis at the human-animal interface in Egypt

Brucellosis is a highly contagious zoonosis affecting humans and a wide range of domesticated and wild animal species. An important element for effective disease containment is to improve knowledge, attitudes and practices (KAP) of afflicted communities. This study aimed to assess the KAP related to brucellosis at the human‐animal interface in an endemic area of Egypt and to identify the risk factors for human infection. A matched case‐control study was conducted at the central fever hospitals located in six governorates in northen Egypt. Face‐to‐face interviews with cases and controls were conducted using a structured questionnaire. In total, 40.7% of the participants owned farm animals in their households. The overall mean practice score regarding animal husbandry, processing and consumption of milk and dairy products was significantly lower among cases compared to controls [‐12.7±18.1 vs 0.68±14.2 respectively; p< 0.001]. Perceived barriers for notification of animal infection/abortion were predominate among cases and positively correlated with participants’ education. The predictors of having brucellosis infection were consumption of unpasteurized milk or raw dairy products and practicing animal husbandry. Applying protective measures against infection significantly reduced its risk. A model predicting risk factors for brucellosis among those who own animal showed that frequent abortions per animal increased the chance for brucellosis infection among human cases by 50‐fold (95% CI: 8.8 – 276.9), whereas the use of protective measures in animal care reduced the odds [OR= 0.11 (95% CI: 0.03 – 0.45)]. In conclusion, consumption of unprocessed dairy products was equally important as contact with infected/aborted animals as major risk factors for Brucella spp. infection among humans in Egypt. There is poor knowledge, negative attitudes and risky behaviors among villagers which can perpetuate the risk of brucellosis transmission at the human‐animal interface. This supports the need for integrating health education into the national brucellosis control program

Ruminant Brucellosis in the Kafr El Sheikh Governorate of the Nile Delta, Egypt: Prevalence of a Neglected Zoonosis

Brucellosis is a zoonosis of mammals caused by bacteria of the genus Brucella. It is responsible for a vast global burden imposed on human health through disability and on animal productivity. In humans brucellosis causes a range of flu-like symptoms and chronic debilitating illness. In livestock brucellosis causes economic losses as a result of abortion, infertility and decreased milk production. The main routes for human infection are consumption of contaminated dairy products and contact with infected ruminants. The control of brucellosis in humans depends on its control in ruminants, for which accurate estimates of the frequency of infection are very useful, especially in areas with no previous frequency estimates. We studied the seroprevalence of brucellosis and its geographic distribution among domestic ruminants in one governorate of the Nile Delta region, Egypt. In the study area, the seroprevalence of ruminant brucellosis is very high and has probably increased considerably since the early 1990s. The disease is widespread but more concentrated around major animal markets. These findings question the efficacy of the control strategy in place and highlight the high infection risk for the animal and human populations of the area and the urgent need for an improved control strategy

Ruminant brucellosis in Egypt : frequency, distribution and potential control strategies

EThOS - Electronic Theses Online ServiceGBUnited Kingdo

High Prevalence of ESBL and Plasmid-Mediated Quinolone Resistance Genes in Salmonella enterica Isolated from Retail Meats and Slaughterhouses in Egypt

The emergence and spread of multidrug-resistant Salmonella enterica (S. enterica) to humans through food of animal origin are considered a major global public health concern. Currently, little is known about the prevalence of important antimicrobial resistance genes in S. enterica from retail food in Africa. Therefore, the screening and characterization of the extended-spectrum β-lactamase (ESBL) and plasmid-mediated quinolone resistance (PMQR) genes in S. enterica isolated from retail meats and slaughterhouses in Egypt were done by using PCR and DNA sequencing techniques. Twenty-eight out of thirty-four (82.4%) non-duplicate S. enterica isolates showed multidrug-resistance phenotypes to at least three classes of antimicrobials, and fourteen (41.2%) exhibited an ESBL-resistance phenotype and harbored at least one ESBL-encoding gene. The identified β-lactamase-encoding genes included blaCTX-M-1, blaCTX-M-3, blaCTX-M-13, blaCTX-M-14, blaCTX-M-15, and blaSHV-12 (ESBL types); blaCMY-2 (AmpC type); and blaTEM-1 and blaOXA-1 (narrow-spectrum types). PMQR genes (included qnrA, qnrB, qnrS, and aac(6′)-Ib-cr) were identified in 23 (67.6%) isolates. The presence of ESBL- and PMQR-producing S. enterica with a high prevalence rate in retail meats and slaughterhouses is considered a major threat to public health as these strains with resistance genes could be transmitted to humans through the food chain

Epidemiological investigation of foot-and-mouth disease outbreak in a vaccinated Egyptian dairy herd with analysis of associated risk factors

This study was conducted to investigate an outbreak of foot-and-mouth disease (FMD) in a vaccinated dairy herd (n = 4,145) and to identify the associated risk factors. Foot-and-mouth disease virus (FMDV) A Asia Iran-05 and SAT2 Libya 12-like viral RNAs were detected in clinical samples. Our data indicates that the outbreak occurred due to introduction of a field virus into cattle with minimal matching protective immune response. Previous vaccination with a multivalent vaccine did not prevent replication of a field virus that is an antigenic match to one of the vaccine seed viruses; with subsequent development of a mixed infection. The total cumulative incidence for the 31-day follow up period was 49.8% and the total mortality rate was 0.8%. The total incidence rate was 21 cases/1,000 cows/day, with confidence interval (CI) 20.32, 22.15. Analysis of epidemiological data revealed that lactation is the primary factor in disease development and mortalities in dairy herds (P < 0.005), possibly due to increased frequency of exposure and higher virus loads. Within this group, cows with 1 parity are more vulnerable in terms of disease development (relative risk 1.2, CI 1.121, 1.285) but not mortalities (P = 0.359). Correlations between FMD development and age should only be considered in the context of the reproductive state. Our analysis revealed that a reduction of the overall disease impact can be achieved by reduction of virus burdens in farms during outbreaks