Orientation of biological cells using plane-polarized Gaussian beam optical tweezers

Optical tweezers are widely used for the manipulation of cells and their internal structures. However, the degree of manipulation possible is limited by poor control over the orientation of trapped cells. We show that it is possible to controllably align or rotate disc shaped cells - chloroplasts of Spinacia oleracea - in a plane polarised Gaussian beam trap, using optical torques resulting predominantly from circular polarisation induced in the transmitted beam by the non-spherical shape of the cells.Comment: 9 pages, 6 figure

Nanotrapping and the thermodynamics of optical tweezers

Particles that can be trapped in optical tweezers range from tens of microns down to tens of nanometres in size. Interestingly, this size range includes large macromolecules. We show experimentally, in agreement with theoretical expectations, that optical tweezers can be used to manipulate single molecules of polyethylene oxide suspended in water. The trapped molecules accumulate without aggregating, so this provides optical control of the concentration of macromolecules in solution. Apart from possible applications such as the micromanipulation of nanoparticles, nanoassembly, microchemistry, and the study of biological macromolecules, our results also provide insight into the thermodynamics of optical tweezers.Comment: 5 pages, 3 figures, presented at 17th AIP Congress, Brisbane, 200

Forces from highly focused laser beams: modeling, measurement and application to refractive index measurements

The optical forces in optical tweezers can be robustly modeled over a broad range of parameters using generalsed Lorenz-Mie theory. We describe the procedure, and show how the combination of experimental measurement of properties of the trap coupled with computational modeling, can allow unknown parameters of the particle - in this case, the refractive index - to be determined.Comment: 5 pages, 4 figures, presented at 17th AIP Congress, Brisbane, 200

Two controversies in classical electromagnetism

This paper examines two controversies arising within classical electromagnetism which are relevant to the optical trapping and micromanipulation community. First is the Abraham-Minkowski controversy, a debate relating to the form of the electromagnetic energy momentum tensor in dielectric materials, with implications for the momentum of a photon in dielectric media. A wide range of alternatives exist, and experiments are frequently proposed to attempt to discriminate between them. We explain the resolution of this controversy and show that regardless of the electromagnetic energy momentum tensor chosen, when material disturbances are also taken into account the predicted behaviour will always be the same. The second controversy, known as the plane wave angular momentum paradox, relates to the distribution of angular momentum within an electromagnetic wave. The two competing formulations are reviewed, and an experiment is discussed which is capable of distinguishing between the two

Observation of generalized synchronization of chaos in a driven chaotic system

We report on the experimental observation of the generalized synchronization of chaos in a real physical system. We show that under a nonlinear resonant interaction, the chaotic dynamics of a single mode laser can become functionally related to that of a chaotic driving signal and furthermore as the coupling strength is further increased, the chaotic dynamics of the laser approaches that of the driving signal.Tang, D. Dykstra, R. ; Hamilton, M. ; Heckenberg, N

Propagation of Arbitrary Non-Paraxial Beams by Expansion in Spherical Functions

While the paraxial approximation is applicable to many, even most, optical systems, the highly non-paraxial regime, where the paraxial approximation and simple corrections to it fail, is becoming increasingly important with the development of intrinsically non-paraxial optical devices and structures such as nano/micro-cavities, photonic crystals, VCSELs, and others of sizes comparable to, or smaller than, the optical wavelength. We calculate the propagation of a highly non-paraxial beam by expansion into spherical functions, which can be considered as fundamental non-paraxial modes. This method is applicable to arbitrary beams