Estimation of land production and its response to cultivated land conversion in North China Plain

Major State Basic Research Development Program of China 2010CB950904;National Natural Science Foundation of China 70503025 40801231;Chinese Academy of Sciences KZCX2-YW-305-2Food safety and its related influencing factors in China are the hot research topics currently, and cultivated land conversion is one of the significant factors influencing food safety in China. Taking the North China Plain as the study area, this paper examines the changes of cultivated land area using satellite images, estimates land productivity from 1985 to 2005 using the model of Estimation System for Land Productivity (ESLP), and analyzes the impact of cultivated land conversion on the land production. Compared with the grain yield data from statistical yearbooks, the results indicate that ESLP model is an effective tool for estimating land productivity. Land productivity in the North China Plain showed a slight decreasing trend from 1985 to 2005, spatially, increased from the north to the south gradually, and the net changes varied in different areas. Cultivated land area recorded a marginal decrease of 8.0 x 10(5) ha, mainly converted to other land uses. Cultivated land conversion had more significant negative impacts on land production than land productivity did. Land production decreased by about 6.48 x 10(6) t caused by cultivated land conversion between 1985 and 2005, accounting for 91.9% of the total land production reduction. Although the land productivity increased in Anhui and Jiangsu provinces, it can not offset the overall adverse effects caused by cultivated land conversion. Therefore, there are significant meanings to control the cultivated land conversion and improve the land productivity for ensuring the land production in the North China Plain

The Importance of Serine Phosphorylation of Ameloblastin on Enamel Formation

FAM20C is a newly identified kinase on the secretory pathway responsible for the phosphorylation of serine residues in the Ser-x-Glu/pSer motifs in several enamel matrix proteins. Fam20C-knockout mice showed severe enamel defects very similar to those in the ameloblastin (Ambn)–knockout mice, implying that phosphoserines may have a critical role in AMBN function. To test this hypothesis, we generated amelogenin (Amel) promoter-driven Ambn-transgenic mice, in which Ser⁴⁸, Ser²²⁶, and Ser²²⁷ were replaced by aspartic acid (designated as D-Tg) or alanines (designated as A-Tg). The negative charge of aspartic acid is believed to be able to mimic the phosphorylation state of serine, while alanine is a commonly used residue to substitute serine due to their similar structure. Using Western immunoblotting and quantitative polymerase chain reaction, the authors identified transgenic lines expressing transgenes somewhat higher (Tg+) or much higher (Tg++) than endogenous Ambn. The lower incisors collected from 7-d-old and 7-wk-old mice were analyzed by histology, scanning electron microscopy, immunohistochemistry, and Western immunoblotting to examine the morphology and microstructure changes in enamel, as well as the expression pattern of enamel matrix proteins. The A-Tg+ and A-Tg++ mice displayed severe enamel defects in spite of the expression level of transgenes, while the D-Tg+ and D-Tg++ mice showed minor to mild enamel defects, indicating that the D-Tg transgenes disturbed enamel formation less than the A-Tg transgenes did. Our results suggest that the phosphorylation state of serines is likely an essential component for the integrity of AMBN function

Sugar alcohols-induced oxidative metabolism in cotton callus culture

Sugar alcohols (mannitol and sorbitol) may cause oxidative damage in plants if used in higher concentration. Our present experiment was undertaken to study physiological and metabolic responses in cotton (Gossypium hirsutum L.) callus against mannitol and sorbitol higher doses. Both markedly declined mean values of relative fresh weight growth rates with the increase in their concentration intensities. The overall protein and malonaldehyde (MDA) contents increased in the stressed-shocked cells. Also, the mean values of various antioxidants such as superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and calalase (CAT) quantitatively improved over their respective controls. As a whole, MDA contents were higher in magnitude than that of different antioxidant enzymes. Also values of relative increase in case of POD were higher as compared to SOD showing the ability of cotton callus culture to scavenge H2O2 produced as a result of the activity of SOD. Our results show that both agents caused greater damage to the membranous structure in comparison to less activation of the antioxidants. As a whole, the overall change regarding fresh weight growth rates was less after 14-day stress regime, while the mean values of the antioxidant enzymes activities were lower after the 28-day stress period. Such decrease conveys the message that less reactive oxygen species (ROS) might have been produced.Keywords: Antioxidants, callus culture, Gossypium hirsutum L., osmotic stress, sugar alcoholsAfrican Journal of Biotechnology Vol. 12(17), pp. 2191-220

Complete analysis of the B-cell response to a protein antigen, from in vivo germinal centre formation to 3-D modelling of affinity maturation

Somatic hypermutation of immunoglobulin variable region genes occurs within germinal centres (GCs) and is the process responsible for affinity maturation of antibodies during an immune response. Previous studies have focused almost exclusively on the immune response to haptens, which may be unrepresentative of epitopes on protein antigens. In this study, we have exploited a model system that uses transgenic B and CD4<sup>+</sup> T cells specific for hen egg lysozyme (HEL) and a chicken ovalbumin peptide, respectively, to investigate a tightly synchronized immune response to protein antigens of widely differing affinities, thus allowing us to track many facets of the development of an antibody response at the antigen-specific B cell level in an integrated system <i>in</i> <i>vivo</i>. Somatic hypermutation of immunoglobulin variable genes was analysed in clones of transgenic B cells proliferating in individual GCs in response to HEL or the cross-reactive low-affinity antigen, duck egg lysozyme (DEL). Molecular modelling of the antibody–antigen interface demonstrates that recurring mutations in the antigen-binding site, selected in GCs, enhance interactions of the antibody with DEL. The effects of these mutations on affinity maturation are demonstrated by a shift of transgenic serum antibodies towards higher affinity for DEL in DEL-cOVA immunized mice. The results show that B cells with high affinity antigen receptors can revise their specificity by somatic hypermutation and antigen selection in response to a low-affinity, cross-reactive antigen. These observations shed further light on the nature of the immune response to pathogens and autoimmunity and demonstrate the utility of this novel model for studies of the mechanisms of somatic hypermutation

Adaptive Functional Diversification of Lysozyme in Insectivorous Bats

This work was supported by the National Natural Science Foundation of China (31172077 to S.Z.), an East China Normal University Fostering Project for National Top Hundred Doctoral Dissertations (PY2011012 to Y.L.), an Innovation Fund of East China Normal University (78210200 to G.H.), and a Biotechnology and Biological Sciences Research Council China Partnering Award (BB/G530392/1 to G.J., S.J.R., and S.Z.)

A novel process for preparing PZT thick films

2000-2001 > Academic research: refereed > Refereed conference paperVersion of RecordPublishe

Transplanted Olfactory Ensheathing Cells Reduce Retinal Degeneration in Royal College of Surgeons Rats

PURPOSE OF THE STUDY: Retinitis pigmentosa (RP) is a group of genetic disorders and a slow loss of vision that is caused by a cascade of retinal degenerative events. We examined whether these retinal degenerative events were reduced after cultured mixtures of adult olfactory ensheathing cells (OECs) and olfactory nerve fibroblasts (ONFs) were transplanted into the subretinal space of 1-month-old RCS rat, a classic model of RP. MATERIALS AND METHODS: The changes in retinal photoreceptors and Müller cells of RCS rats after cell transplantation were observed by the expression of recoverin and glial fibrillary acidic protein (GFAP), counting peanut agglutinin (PNA)-positive cone outer segments and calculating the relative apoptotic area. The retinal function was also evaluated by Flash electroretinography (ERG). To further investigate the mechanisms, by which OECs/ONFs play important roles in the transplanted retinas, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and basic fibroblast growth factor (bFGF) secretion of the cultured cells were analyzed by ELISA. The ability of OECs/ONFs to ingest porcine retinal outer segments and the amount of phagocytosis were compared with retinal pigment epithelium (RPE) cells. RESULTS: Our research showed that the transplantation of OECs/ONFs mixtures restored recoverin expression, protected retinal outer segments, increased PNA-positive cone outer segments, reduced caspase-positive apoptotic figures, downregulated GFAP, and maintained the b-wave of the ERG. Cultured OECs/ONFs expressed and secreted NGF, BDNF, and bFGF which made contributions to assist survival of the photoreceptors. An in vitro phagocytosis assay showed that OECs, but not ONFs, phagocytosed porcine retinal outer segments, and the phagocytic ability of OECs was even superior to that of RPE cells. CONCLUSIONS: These findings demonstrate that transplantation of OECs/ONFs cleaned up the accumulated debris in subretinal space, and provided an intrinsic continuous supply of neurotrophic factors. It suggested that transplantation of OECs/ONFs might be a possible future route for protection of the retina and reducing retinal degeneration in RP

Herschel Hi-GAL imaging of massive young stellar objects

We used Herschel Hi-GAL (Herschel infrared Galactic Plane survey) data to determine whether massive young stellar objects (MYSOs) are resolved at 70 μm and to study their envelope density distribution. Our analysis of three relatively isolated sources in the l = 30° and 59° Galactic fields show that the objects are partially resolved at 70 μm. The Herschel Hi-GAL survey data have a high scan velocity which makes unresolved and partially resolved sources appear elongated in the 70 μm images. We analysed the two scan directions separately and examine the intensity profile perpendicular to the scan direction. Spherically symmetric radiative transfer models with a power-law density distribution were used to study the circumstellar matter distribution. Single dish submm data were also included to study how different spatial information affects the fitted density distribution. The density distribution which best fits both the 70 μm intensity profile and spectral energy distribution has an average index of ∼0.5. This index is shallower than expected and is probably due to the dust emission from bipolar outflow cavity walls not accounted for in the spherical models. We conclude that 2D axisymmetric models and Herschel images at low scan speeds are needed to better constrain the matter distribution around MYSOs

Seed systems smallholder farmers use

Seed can be an important entry point for promoting productivity, nutrition and resilience among smallholder farmers. While investments have primarily focused on strengthening the formal sector, this article documents the degree to which the informal sector remains the core for seed acquisition, especially in Africa. Conclusions drawn from a uniquely comprehensive data set, 9660 observations across six countries and covering 40 crops, show that farmers access 90.2 % of their seed from informal systems with 50.9 % of that deriving from local markets. Further, 55 % of seed is paid for by cash, indicating that smallholders are already making important investments in this arena. Targeted interventions are proposed for rendering formal and informal seed sector more smallholder-responsive and for scaling up positive impacts