Over-expression of human cytomegalovirus miR-US25-2-3p downregulates eIF4A1 and inhibits HCMV replication

AbstractIt has been reported that human cytomegalovirus (HCMV) miR-US25-2 reduces DNA viral replication including HCMV. However, the mechanism remains unknown. In our study, eukaryotic translation initiation factor 4A1 (eIF4A1) was identified to be a direct target of miR-US25-2-3p. Small interfering RNA (siRNA) and miR-US25-2-3p mediated eIF4A1 knockdown experiments revealed that high level of miR-US25-2-3p in MRC-5 cells decreased HCMV and host genomic DNA synthesis, and inhibited cap-dependent translation and host cell proliferation. However, eIF4A1 up-regulation induced by miR-US25-2-3p inhibitor increased HCMV copy number. Therefore, the over-expression of miR-US25-2-3p and consequent lower expression of eIF4A1 may contribute to the inhibition of HCMV replication

The comprehensive environmental efficiency of socioeconomic sectors in China: An analysis based on a non-separable bad output SBM

The increasingly high frequency of heavy air pollution in most regions of China signals the urgent need for the transition to an environmentally friendly production performance by socioeconomic sectors for the sake of people's health and sustainable development. Focusing on CO2 and major air pollutants, this paper presents a comprehensive environmental efficiency index based on evaluating the environmental efficiency of major socioeconomic sectors, including agriculture, power, industry, residential and transportation, at the province level in China in 2010 based on a slack-based measure DEA model with non-separable bad output and weights determined by the coefficient of variation method. In terms of the environment, 5, 16, 6, 7 and 4 provinces operated along the production frontier for the agricultural, power, industrial, residential and transportation sectors, respectively, in China in 2010, whereas Shanxi, Heilongjiang, Ningxia, Hubei and Yunnan showed lowest efficiency correspondingly. The comprehensive environmental efficiency index varied from 0.3863 to 0.9261 for 30 provinces in China, with a nationwide average of 0.6383 in 2010; Shanghai ranked at the top, and Shanxi was last. Regional disparities in environmental efficiency were identified. A more detailed inefficiency decomposition and benchmarking analysis provided insight for understanding the source of comprehensive environmental inefficiency and, more specifically, the reduction potential for CO2 and air pollutants. Some specific research and policy implications were uncovered from this work

Characteristics and sources of water-soluble organic aerosol in a heavily polluted environment in Northern China

Water-soluble organic aerosol (WSOA) in fine particles (PM2.5) collected during wintertime in a polluted city (Handan) in Northern China was characterized using a High-Resolution Time-of-Flight Aerosol Mass Spectrometer (AMS). Through comparing with real-time measurements from a collocated Aerosol Chemical Speciation Monitor (ACSM), we determined that WSOA on average accounts for 29% of total organic aerosol (OA) mass and correlates tightly with secondary organic aerosol (SOA; Pearson's r = 0.95). The mass spectra of WSOA closely resemble those of ambient SOA, but also show obvious influences from coal combustion and biomass burning. Positive matrix factorization (PMF) analysis of the WSOA mass spectra resolved a water-soluble coal combustion OA (WS-CCOA; O/C = 0.17), a water-soluble biomass burning OA (WS-BBOA; O/C = 0.32), and a water-soluble oxygenated OA (WS-OOA; O/C = 0.89), which account for 10.3%, 29.3% and 60.4% of the total WSOA mass, respectively. The water-solubility of the OA factors was estimated by comparing the offline AMS analysis results with the ambient ACSM measurements. OOA has the highest water-solubility of 49%, consistent with increased hygroscopicity of oxidized organics induced by atmospheric aging processes. In contrast, CCOA is the leastwater soluble, containing 17% WS-CCOA. The distinct characteristics of WSOA from different sources extend our knowledge of the complex aerosol chemistry in the polluted atmosphere of Northern China and the water-solubility analysis may help us to understand better aerosol hygroscopicity and its effects on radiative forcing in this region. (C) 2020 Published by Elsevier B.V.Peer reviewe

Antioxidant Supplement Inhibits Skeletal Muscle Constitutive Autophagy rather than Fasting-Induced Autophagy in Mice

In this study, we tested the hypothesis that NAC administration leads to reduced oxidative stress and thus to decreased expression of autophagy markers in young mice. Our results reveal that NAC administration results in reduced muscle mRNA levels of several autophagy markers, including Beclin-1, Atg7, LC3, Atg9, and LAMP2. However, NAC supplement fails to block the activation of skeletal muscle autophagy in response to fasting, because fasting significantly increases the mRNA level of several autophagy markers and LC3 lipidation. We further examined the effects of NAC administration on mitochondrial antioxidant capacity in fed and 24-hour fasted mice. Our results clearly show that NAC administration depresses the expression of manganese superoxide dismutase (MnSOD) and TP53-induced glycolysis and apoptosis regulator (TIGAR), both of which play a predominant antioxidant role in mitochondria by reducing ROS level. In addition, we found no beneficial effect of NAC supplement on muscle mass but it can protect from muscle loss in response to fasting. Collectively, our findings indicate that ROS is required for skeletal muscle constitutive autophagy, rather than starvation-induced autophagy, and that antioxidant NAC inhibits constitutive autophagy by the regulation of mitochondrial ROS production and antioxidant capacity

Arctic Ocean Simulations in the CMIP6 Ocean Model Intercomparison Project (OMIP)

oai:publications.copernicus.org:gmdd107357Arctic Ocean simulations in 19 global ocean-sea ice models participating in the Ocean Model Intercomparison Project (OMIP) of the CMIP6 are evaluated in this paper. Our results indicate that no significant improvements were achieved in the Arctic Ocean simulations from the previous Coordinated Ocean-ice Reference Experiments phase II (CORE-II) to the current OMIP. Large model biases and inter-model spread exist in the simulated mean state of the halocline and Atlantic Water layer in the OMIP models. Most of the OMIP models suffer from too thick and deep Atlantic Water layer, too deep halocline base, and large fresh biases in the halocline. The OMIP models largely agree on the inter-annual and decadal variability of the Arctic Ocean freshwater content and volume/heat/freshwater transports through the Arctic Ocean gateways. The models can reproduce observed changes in volume, heat and freshwater transports through the gateways except for the Bering Strait. Overall, the performance of the Arctic Ocean simulations is similar between the CORE2-forced OMIP-1 and JRA55-do-forced OMIP-2.</p

Induction of PtoCDKB and PtoCYCB transcription by temperature during cambium reactivation in Populus tomentosa Carr.

Cell cycle progression requires interaction between cyclin-dependent kinase B (CDKB) and cyclin B (CYCB). The seasonal expression patterns of the CDKB and CYCB homologues from Populus tomentosa Carr. were investigated, and effects of temperature and exogenous indole-3-acetic acid (IAA) on their expression were further studied in water culture experiments. Based on the differential responses of dormant cambium cells to exogenous IAA, four stages of cambium dormancy were confirmed for P. tomentosa: quiescence 1 (Q1), rest, quiescence 2-1 (Q2-1), and quiescence 2-2 (Q2-2). PtoCDKB and PtoCYCB transcripts were strongly expressed in the active phases, weakly in Q1, and almost undetectable from rest until late Q2-2. Climatic data analysis showed a correlation between daily air temperature and PtoCDKB and PtoCYCB expression patterns. Water culture experiments with temperature treatment further showed that a low temperature (4 °C) kept PtoCDKB and PtoCYCB transcripts at undetectable levels, while a warm temperature (25 °C) induced their expression in the cambium region. Meanwhile, water culture experiments with exogenous IAA treatment showed that induction of PtoCDKB and PtoCYCB transcription was independent of exogenous IAA. The results suggest that, in deciduous hardwood P. tomentosa growing in a temperate zone, the temperature in early spring is a vital environmental factor for cambium reactivation. The increasing temperature in early spring may induce CDKB and CYCB homologue transcription in the cambium region, which is necessary for cambium cell division

An IoT-oriented data placement method with privacy preservation in cloud environment

© 2018 Elsevier Ltd IoT (Internet of Things) devices generate huge amount of data which require rich resources for data storage and processing. Cloud computing is one of the most popular paradigms to accommodate such IoT data. However, the privacy conflicts combined in the IoT data makes the data placement problem more complicated, and the resource manager needs to take into account the resource efficiency, the power consumption of cloud data centers, and the data access time for the IoT applications while allocating the resources for the IoT data. In view of this challenge, an IoT-oriented Data Placement method with privacy preservation, named IDP, is designed in this paper. Technically, the resource utilization, energy consumption and data access time in the cloud data center with the fat-tree topology are analyzed first. Then a corresponding data placement method, based on the Non-dominated Sorting Genetic Algorithm II (NSGA-II), is designed to achieve high resource usage, energy saving and efficient data access, and meanwhile realize privacy preservation of the IoT data. Finally, extensive experimental evaluations validate the efficiency and effectiveness of our proposed method