Oxidized guanine base lesions function in 8-oxoguanine DNA glycosylase-1-mediated epigenetic regulation of nuclear factor κB-driven gene expression

A large percentage of redox-responsive gene promoters contain evolutionarily conserved guanine-rich clusters; guanines are the bases most susceptible to oxidative modification(s). Consequently, 7,8-dihydro-8-oxoguanine (8-oxoG) is one of the most abundant base lesions in promoters and is primarily repaired via the 8-oxoguanine DNA glycosylase-1 (OOG1)-initiated base excision repair pathway. In view of a prompt cellular response to oxidative challenge, we hypothesized that the 8-oxoG lesion and the cognate repair protein OGG1 are utilized in transcriptional gene activation. Here, we document TNFα-induced enrichment of both 8-oxoG and OGG1 in promoters of pro-inflammatory genes, which precedes interaction of NF-κB with its DNA-binding motif. OGG1 bound to 8-oxoG upstream from the NF-κB motif increased its DNA occupancy by promoting an on-rate of both homodimeric and heterodimeric forms of NF-κB. OGG1 depletion decreased both NF-κB binding and gene expression, whereas Nei-like glycosylase-1 and -2 had a marginal effect. These results are the first to document a novel paradigm wherein the DNA repair protein OGG1 bound to its substrate is coupled to DNA occupancy of NF-κB and functions in epigenetic regulation of gene expression. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc

Not Available

Not AvailableNot AvailableNot Availabl

Effects of the stimuli-dependent enrichment of 8-oxoguanine DNA glycosylase1 on chromatinized DNA

8-Oxoguanine DNA glycosylase 1 (OGG1) initiates the base excision repair pathway by removing one of the most abundant DNA lesions, 8-oxo-7,8-dihydroguanine (8-oxoG). Recent data showed that 8-oxoG not only is a pro-mutagenic genomic base lesion, but also functions as an epigenetic mark and that consequently OGG1 acquire distinct roles in modulation of gene expression. In support, lack of functional OGG1 in Ogg1-/- mice led to an altered expression of genes including those responsible for the aberrant innate and adaptive immune responses and susceptibility to metabolic disorders. Therefore, the present study examined stimulus-driven OGG1-DNA interactions at whole genome level using chromatin immunoprecipitation (ChIP)-coupled sequencing, and the roles of OGG1 enriched on the genome were validated by molecular and system-level approaches. Results showed that signaling levels of cellular ROS generated by TNFα induced enrichment of OGG1 at specific sites of chromatinized DNA, primarily in the regulatory regions of genes. OGG1-ChIP-ed genes are associated with important cellular and biological processes and OGG1 enrichment was limited to a time scale required for immediate cellular responses. Prevention of OGG1-DNA interactions by siRNA depletion led to modulation of NF-κB's DNA occupancy and differential expression of genes. Taken together these data show TNFα-ROS-driven enrichment of OGG1 at gene regulatory regions in the chromatinized DNA, which is a prerequisite to modulation of gene expression for prompt cellular responses to oxidant stress. © 201

Involvement of Plant Hormones and Plant Growth Regulators on in vitro Somatic Embryogenesis

In spite of the importance attained by somatic embryogenesis and of the many studies that have been conducted on this developmental process, there are still many aspects that are not fully understood. Among those features, the involvement of plant hormones and plant growth regulators on deTermining the conversion of somatic onto embryogenic tissues, and on allowing progression and maturation of somatic embryos, are far away from being completely comprehended. Part of these difficulties relies on the frequent appearance of contradictory results when studying the effect of a particular stimulus over a specific stage in somatic embryogenesis. Recent progress achieved on understanding the interaction between exogenously added plant growth regulators over the concentration of endogenous hormones, together with the involvement of sensitivity of the tissues to particular hormone groups, might help clarifying the occurrence of divergent patterns in somatic embryogenesis, and in tissue culture in general. The aspects described above, emphasizing on the effect of the concentration of plant hormones and of the addition of plant growth regulators during the different phases of somatic embryogenesis, will be reviewed in this paper. Citations will be limited to review articles as much as possible and to individual articles only in those cases in which very specific or recent information is presented.UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS

Study of χbJ(nP)→ωΥ(1S)\chi_{bJ}(nP) \rightarrow \omega \Upsilon(1S) at Belle

We report results from a study of hadronic transitions of the $\chi_{bJ}(nP)$ states of bottomonium at Belle. The $P$-wave states are reconstructed in transitions to the $\Upsilon(1S)$ with the emission of an $\omega$ meson. The transitions of the $n=2$ triplet states provide a unique laboratory in which to study nonrelativistic quantum chromodynamics, as the kinematic threshold for production of an $\omega$ and $\Upsilon(1S)$ lies between the $J=0$ and $J=1$ states. A search for the $\chi_{bJ}(3P)$ states is also reported

Measurement of the BB−^{-} →\rightarrow DD0^{0}ℓ\ell−^{-}νˉ\bar{\nu}ℓ_{\ell} Branching Fraction in 62.8 fb−1^{-1} of Belle II data

We report a measurement of the branching fraction of the semileptonic decay $B$$^{-}$ $\rightarrow$ $D$$^{0}$$\ell$$^{-}$$\bar{\nu}$$_{\ell}$ (and its charge conjugate) using 62.8 fb$^{-1}$ of $\Upsilon$(4$S$) $\rightarrow$ $B$$\bar{B}$ data recorded by the Belle II experiment at the SuperKEKB asymmetric-energy $e$$^{+}$ $e$$^{-}$ collider. The neutral charm meson is searched for in the decay mode $D$$^{0}$ $\rightarrow$ $K$$^{-}$ $\pi$$^{+}$ and combined with a properly charged identified lepton (electron or muon) to reconstruct this decay. No reconstruction of the second $B$ meson in the $\Upsilon$(4$S$) event is performed. We obtain $B$($D$$^{0}$$\ell$$^{-}$$\bar{\nu}$$_{\ell}$) = (2.29 $\pm$ 0.05 $_{stat}$ $\pm$ 0.08$_{syst}$, in agreement with the world average of this decay. We also determine the ratio of the electron to muon branching fractions to be $R$($e$/$\mu$) = 1.04 $\pm$ 0.05$_{stat}$ $\pm$ 0.03$_{syst}$ and observe no deviation from lepton universality