Lactococcus lactis subsp. lactis as a natural anti-listerial agent in the mushroom industry

peer-reviewedMushroom growth substrates from different commercial producers of mushrooms (Agaricus bisporus) were screened for the presence of bacteria with potential for use as biocontrol agents for controlling Listeria monocytogenes in the mushroom production environment. Eight anti-listerial strains were isolated from different sources and all were identified using 16s rRNA gene sequencing as Lactococcus lactis subsp. lactis. Whole-genome sequencing of the Lc. lactis isolates indicated that strains from different sites and substrate types were highly similar. Colony MALDI-TOF mass spectrometry found that these strains were Nisin Z producers but inhibitory activity was highly influenced by the incubation conditions and was strain dependant. The biofilm forming ability of these strains was tested using a crystal violet assay and all were found to be strong biofilm formers. Growth of Lc. lactis subsp. lactis using mixed-biofilm conditions with L. monocytogenes on stainless steel resulted in a 4-log reduction of L. monocytogenes cell numbers. Additional sampling of mushroom producers showed that these anti-listerial Lc. lactis strains are commonly present in the mushroom production environment. Lc. lactis has a generally regarded as safe (GRAS) status and therefore has potential for use as an environmentally benign solution to control L. monocytogenes in order to prevent product contamination and to enhance consumer confidence in the mushroom industry

Mount St. Helens aerosol evolution

Stratospheric aerosol samples were collected using a wire impactor during the year following the eruption of Mount St. Helens. Analysis of samples shows that aerosol volume increased for 6 months due to gas-to-particle conversion and then decreased to background levels in the following 6 months

Neutral gas in Lyman-alpha emitting galaxies Haro 11 and ESO 338-IG04 measured through sodium absorption

Context. The Lyman alpha emission line of galaxies is an important tool for finding galaxies at high redshift, and thus probe the structure of the early universe. However, the resonance nature of the line and its sensitivity to dust and neutral gas is still not fully understood. Aims. We present measurements of the velocity, covering fraction and optical depth of neutral gas in front of two well known local blue compact galaxies that show Lyman alpha in emission: ESO 338-IG 04 and Haro 11. We thus test observationally the hypothesis that Lyman alpha can escape through neutral gas by being Doppler shifted out of resonance. Methods. We present integral field spectroscopy from the GIRAFFE/Argus spectrograph at VLT/FLAMES in Paranal, Chile. The excellent wavelength resolution allows us to accurately measure the velocity of the ionized and neutral gas through the H-alpha emission and Na D absorption, which traces the ionized medium and cold interstellar gas, respectively. We also present independent measurements with the VLT/X-shooter spectrograph which confirm our results. Results. For ESO 338-IG04, we measure no significant shift of neutral gas. The best fit velocity is -15 (16) km/s. For Haro 11, we see an outflow from knot B at 44 (13) km/s and infalling gas towards knot C with 32 (12) km/s. Based on the relative strength of the Na D absorption lines, we estimate low covering fractions of neutral gas (down to 10%) in all three cases. Conclusions. The Na D absorption likely occurs in dense clumps with higher column densities than where the bulk of the Ly-alpha scattering takes place. Still, we find no strong correlation between outflowing neutral gas and a high Lyman alpha escape fraction. The Lyman alpha photons from these two galaxies are therefore likely escaping due to a low column density and/or covering fraction.Comment: 9 pages, 3 figure

Coherent Error Suppression in Multi-Qubit Entangling Gates

We demonstrate a simple pulse shaping technique designed to improve the fidelity of spin-dependent force operations commonly used to implement entangling gates in trapped-ion systems. This extension of the M{\o}lmer-S{\o}rensen gate can theoretically suppress the effects of certain frequency and timing errors to any desired order and is demonstrated through Walsh modulation of a two-qubit entangling gate on trapped atomic ions. The technique is applicable to any system of qubits coupled through collective harmonic oscillator modes

A Search for Intrinsic Polarization in O Stars with Variable Winds

New observations of 9 of the brightest northern O stars have been made with the Breger polarimeter on the 0.9~m telescope at McDonald Observatory and the AnyPol polarimeter on the 0.4~m telescope at Limber Observatory, using the Johnson-Cousins UBVRI broadband filter system. Comparison with earlier measurements shows no clearly defined long-term polarization variability. For all 9 stars the wavelength dependence of the degree of polarization in the optical range can be fit by a normal interstellar polarization law. The polarization position angles are practically constant with wavelength and are consistent with those of neighboring stars. Thus the simplest conclusion is that the polarization of all the program stars is primarily interstellar. The O stars chosen for this study are generally known from ultraviolet and optical spectroscopy to have substantial mass loss rates and variable winds, as well as occasional circumstellar emission. Their lack of intrinsic polarization in comparison with the similar Be stars may be explained by the dominance of radiation as a wind driving force due to higher luminosity, which results in lower density and less rotational flattening in the electron scattering inner envelopes where the polarization is produced. However, time series of polarization measurements taken simultaneously with H-alpha and UV spectroscopy during several coordinated multiwavelength campaigns suggest two cases of possible small-amplitude, periodic short-term polarization variability, and therefore intrinsic polarization, which may be correlated with the more widely recognized spectroscopic variations.Comment: LaTeX2e, 22 pages including 11 tables; 12 separate gif figures; uses aastex.cls preprint package; accepted by The Astronomical Journa

The impact of broadband in schools

The report reviews evidence for the impact of broadband in English schools, exploring; Variations in provision in level of broadband connectivity; Links between the level of broadband activity and nationally accessible performance data; Aspects of broadband connectivity and the school environment that contribute to better outcomes for pupils and teachers; Academic and motivational benefits associated with educational uses of this technology

The role of the individual in the coming era of process-based therapy

For decades the development of evidence-based therapy has been based on experimental tests of protocols designed to impact psychiatric syndromes. As this paradigm weakens, a more process-based therapy approach is rising in its place, focused on how to best target and change core biopsychosocial processes in specific situations for given goals with given clients. This is an inherently more idiographic question than has normally been at issue in evidence-based therapy over the last few decades. In this article we explore methods of assessment and analysis that can integrate idiographic and nomothetic approaches in a process-based era.Accepted manuscrip

Optical BCS conductivity at imaginary frequencies and dispersion energies of superconductors

We present an efficient expression for the analytic continuation to arbitrary complex frequencies of the complex optical and AC conductivity of a homogeneous superconductor with arbitrary mean free path. Knowledge of this quantity is fundamental in the calculation of thermodynamic potentials and dispersion energies involving type-I superconducting bodies. When considered for imaginary frequencies, our formula evaluates faster than previous schemes involving Kramers--Kronig transforms. A number of applications illustrates its efficiency: a simplified low-frequency expansion of the conductivity, the electromagnetic bulk self-energy due to longitudinal plasma oscillations, and the Casimir free energy of a superconducting cavity.Comment: 20 pages, 7 figures, calculation of Casimir energy adde

Twenty eight years of ICP Vegetation: an overview of its activities

Here we look back at the activities and achievements in the 28 years of the International Cooperative Programme on the Effects of Air Pollution on Natural Vegetation and Crops (ICP Vegetation). The ICP Vegetation is a subsidiary body of the Working Group on Effects of the UNECE Convention on Long-range Transboundary Air Pollution (LTRAP), established in 1979. An important role of the ICP Vegetation is to provide evidence for air pollution impacts on vegetation in support of policy development and review of the LRTAP Convention and its Protocols. The activities and participation in the ICP Vegetation have grown over the years. The main activities include: • Collate evidence of ozone impacts on vegetation, assess spatial patterns and temporal trends across Europe; • Develop dose-response relationships, establish critical levels for vegetation and provide European risk maps of ozone impacts; • Reviewing the literature on ozone impacts on vegetation and produce thematic scientific reports and policy-relevant brochures; • Determine spatial patterns and temporal trends of heavy metals, nitrogen and persistent organic pollutants concentrations in mosses as a biomonitoring tool of atmospheric deposition of these compounds