Large Winding Sector of AdS/CFT

We study a family of classical strings on R x S^3 subspace of the AdS_5 x S^5 background that interpolates between pulsating strings and single-spike strings. They are obtained from the helical strings of hep-th/0609026 by interchanging worldsheet time and space coordinates, which maps rotating/spinning string states with large spins to oscillating states with large winding numbers. From a finite-gap perspective, this transformation is realised as an interchange of quasi-momentum and quasi-energy defined for the algebraic curve. The gauge theory duals are also discussed, and are identified with operators in the non-holomorphic sector of N=4 super Yang-Mills. They can be viewed as excited states above the ``antiferromagnetic'' state, which is ``the farthest from BPS'' in the spin-chain spectrum. Furthermore, we investigate helical strings on AdS_3 x S^1 in an appendix.Comment: 1+52 pages, 10 figures, v2: references and comments added, v3: minor changes and a reference adde

Revisiting the Effect of Branch Handling Strategies on Change Recommendation

Although literature has noted the effects of branch handling strategies on change recommendation based on evolutionary coupling, they have been tested in a limited experimental setting. Additionally, the branches characteristics that lead to these effects have not been investigated. In this study, we revisited the investigation conducted by Kovalenko et al. on the effect to change recommendation using two different branch handling strategies: including changesets from commits on a branch and excluding them. In addition to the setting by Kovalenko et al., we introduced another setting to compare: extracting a changeset for a branch from a merge commit at once. We compared the change recommendation results and the similarity of the extracted co-changes to those in the future obtained using two strategies through 30 open-source software systems. The results show that handling commits on a branch separately is often more appropriate in change recommendation, although the comparison in an additional setting resulted in a balanced performance among the branch handling strategies. Additionally, we found that the merge commit size and the branch length positively influence the change recommendation results.Comment: 11 pages, ICPC 202

Evaluating the Raftophilicity of Rhodopsin Photoreceptor in a Patterned Model Membrane

AbstractLipid rafts in the cell membrane are believed to affect various membrane functions, including the signaling by G-protein coupled receptors (GPCRs). However, the regulatory roles of lipid rafts on GPCRs’ functions are still poorly understood, partially owing to the lack of the methods to quantitatively evaluate the affinity of membrane proteins to lipid raft (raftophilicity). Here, we describe a methodology to gauge the raftophilicity of a representative GPCR in vertebrate photoreceptor, i.e., rhodopsin (Rh), and its cognate G protein transducin (Gt) by using a patterned model membrane. We generated a substrate-supported planar lipid bilayer that has patterned regions of liquid-ordered (Lo) and liquid-disordered (Ld) membrane domains. We reconstituted Rh and Gt into the patterned membrane and observed their lateral distribution and diffusion. Mobile and functional Rh molecules could be reconstituted through the rapid dilution of solubilized Rh, by optimizing the reconstitution conditions including the chamber design, protein/detergent concentrations, and solution mixing. We determined the partition and diffusion coefficients of Rh and Gt in the Lo-rich and Ld-rich regions. Both Rh and Gt were predominantly localized in the Ld phase, suggesting their low affinity to lipid rafts. Patterned model membrane offers a robust and scalable platform for systematically and quantitatively studying the functional roles of lipid rafts in biological membranes including retinal disk membranes

Macromolecular lactate dehydrogenase linked to serum IgG of a patient with liver cirrhosis

An anomalous zymogram of lactate dehydrogenase (LDH) in the serum from a patient with liver cirrhosis was reported. Agar-gel electrophoresis of serum showed an extra LDH band close to the anodic side of LDH5 and a wide band of LDH5. Gel filtration of patient's serum in Sephadex G-200 demonstrated an abnormal LDH fraction eluted between immunoglobulin G (IgG) and macroglobulin in addition to a normal LDH component. Chromatographically abnormal LDH was demonstrated on agar gel as extra and wide LDH5 bands and resembled closely human hepatic LDH in various physico-chemical properties such as inhibition by urea or substrate, stability against heat, and Michaelis-Menten's constant. Immunological analyses demonstrated that abnormal LDH could be in the state combined with IgG. Molecular weight of the complex estimated by gel filtration was approximately 300,000. Mixtures of the heated patient's serum with normal or patient's hepatic LDH showed abnormal LDH fraction by gel filtration, whereas abnormal fraction was not demonstrated when heated normal serum was mixed with normal or the patient's hepatic LDH. These results strongly suggest that the occurrence of anomalous LDH zymogram in patient's serum is due to a formation of LDH-IgG complex, which is based on the binding of essentially normal hepatic LDH and abnormal IgG.</p

Substrate binding induces structural changes in cytochrome P450cam

X-ray structures of ferric cytochrome P450cam partially complexed with the substrate (+)-camphor to two different extents were determined at 1.30–1.35 Å resolution, revealing the protein structures of the substrate-free and substrate-bound forms

Leisure-time, occupational, and commuting physical activity and risk of type 2 diabetes in Japanese workers: a cohort study

Table S1. Association between moderate-intensity and vigorous-intensity exercise during leisure and risk of type 2 diabetes. Table S2. Risk of type 2 diabetes associated with specific type of leisure-time exercise. (DOCX 47 kb