Nitrous Oxide Emissions

End of project reportNitrous oxide (N2O) is one of the three most important greenhouse gases (GHG). Nitrous oxide emissions currently account for approximately one third of GHG emissions from agriculture in Ireland. Emissions of N2O arise naturally from soil sources and from the application of nitrogen (N) in the form of N fertilizers and N in dung and urine deposition by grazing animals at pasture. Nitrous oxide emission measurements were conducted at three different scales. Firstly, a large-scale field experiment was undertaken to compare emission rates from a pasture receiving three different rates of N fertilizer application and to identify the effects of controlling variables over a two-year period. Variation in emission rates was large both within and between years. Two contrasting climatic years were identified. The cooler and wetter conditions in year 1 gave rise to considerably lower emission levels than the warmer and drier year 2. However, in both years, peak emissions were associated with fertilizer N applications coincident with rainfall events in the summer months. A small-plot study was conducted to identify the individual and combined effects of fertilizer, dung and urine applications to grassland. Treatment effects were however, difficult to obtain due to the overriding effects of environmental variables. Thirdly, through the use of a small-scale mini-lysimeter study, the diurnal nature of N2O emission rates was identified for two distinct periods during the year. The occurrence of a diurnal pattern has important implications for the identification of a measurement period during the day which is representative of the true daily flux. The research presented aims to identify the nature and magnitude of N2O emissions and the factors which affect emission rates from a grassland in Ireland. Further work is required to integrate the effects of different soil types and contrasting climatic regimes across soil types on N2O emissions.Environmental Protection Agenc

Exploring optimistic and pessimistic attributions in depression-specific mood

Cognitive theories of depression include maladaptive thinking styles as depressive vulnerabilities. The hopelessness theory of depression (Abramson, Metalsky, & Alloy, 1989) particularly implicates stable and global attributions for negative events as influences upon depression. Positive event attributions are considered less influential, yet they have shown equal predictiveness to negative event attributions for depression-specific mood. Previous research has provided equivocal results largely because of cross-sectional design and modest psychometric properties of the measures. The present research aimed to: create a new instrument to measure optimistic and pessimistic attributions; test the relatedness of attributions for positive and negative events; and, clarify relationships of the scales with optimism and mood. Three studies were undertaken, all of which used structural equation modeling. Two cross-sectional studies, using 342 and 332 community participants respectively, developed and validated the Questionnaire of Explanatory Style (QES). A final longitudinal study with 250 community participants tested the predictive validity of the QES. Overall, six scales were developed, three of which were optimistic and three of which were negative. The scales were acceptable to community samples and had adequate psychometric properties. The optimistic scales were attributions for positive events and the negative scales were attributions for negative events rather than pessimistic scales. Cross-sectional results indicated that only one of the negative scales weakly directly predicted depression-specific mood, but all predicted general psychological distress. By contrast, the optimistic scales were more directly predictive of depression-specific mood, particularly the Positive Disposition scale. Longitudinal results indicated that two of the optimistic scales were the most important QES predictors of depression-specific mood two months later. The optimistic scale Positive Disposition appears most central to the prediction of both concurrent and subsequent depression-specific mood. The scale content represents explanations for positive events that are internal and stable characteristics. These may be construed as personal competencies to bring about positive outcomes. This scale is closely allied to measures of optimism. Findings affirm the importance of optimistic attributions to the understanding of depression-specific mood and provide a productive focus for therapeutic intervention and future research

The construct validity of brief continence self-assessment awareness questionnaires

The aim of this study was to develop brief versions of the continence self-assessment awareness questionnaire (CSAAQ), sensitive to gender-specific experiences of incontinence, and to evaluate their construct validity. Recruitment took place at four Victorian healthcare settings during 2001 and 2002. A total of 431 participants completed the CSAAQ following an appointment or hospital discharge. Factor analyses performed on CSAAQ items demonstrated there were three factors (urinary incontinence symptoms, faecal incontinence symptoms, lifestyle adjustment behaviours) for both women and men. The urinary and faecal items that loaded on their respective factors were different for women and men. Consequently two versions of the CSAAQ were developed; one for males and one for females. Analyses supported the construct validity and internal consistency reliability of the gender specific questionnaires. The brief gender specific CSAAQs could be produced as a single questionnaire with separate sections for female and male specific items. The brief CSAAQs are suitable for use as an incontinence self-assessment questionnaire to increase awareness of individual incontinence issues and to encourage health-seeking behaviours.<br /

Cannabigerolic acid, a major biosynthetic precursor molecule in cannabis, exhibits divergent effects on seizures in mouse models of epilepsy

Background and Purpose: Cannabis has been used to treat epilepsy for millennia, with such use validated by regulatory approval of cannabidiol (CBD) for Dravet syndrome. Unregulated artisanal cannabis-based products used to treat children with intractable epilepsies often contain relatively low doses of CBD but are enriched in other phytocannabinoids. This raises the possibility that other cannabis constituents might have anticonvulsant properties. Experimental Approach: We used the Scn1a+/− mouse model of Dravet syndrome to investigate the cannabis plant for phytocannabinoids with anticonvulsant effects against hyperthermia-induced seizures. The most promising, cannabigerolic acid (CBGA), was further examined against spontaneous seizures and survival in Scn1a+/− mice and in electroshock seizure models. Pharmacological effects of CBGA were surveyed across multiple drug targets. Key Results: The initial screen identified three phytocannabinoids with novel anticonvulsant properties: CBGA, cannabidivarinic acid (CBDVA) and cannabigerovarinic acid (CBGVA). CBGA was most potent and potentiated the anticonvulsant effects of clobazam against hyperthermia-induced and spontaneous seizures, and was anticonvulsant in the MES threshold test. However, CBGA was proconvulsant in the 6-Hz threshold test and a high dose increased spontaneous seizure frequency in Scn1a+/− mice. CBGA was found to interact with numerous epilepsy-relevant targets including GPR55, TRPV1 channels and GABAA receptors. Conclusion and Implications: These results suggest that CBGA, CBDVA and CBGVA may contribute to the effects of cannabis-based products in childhood epilepsy. Although these phytocannabinoids have anticonvulsant potential and could be lead compounds for drug development programmes, several liabilities would need to be overcome before CBD is superseded by another in this class

Sexual Phenotype Differences in zic2 mRNA Abundance in the Preoptic Area of a Protogynous Teleost, Thalassoma bifasciatum

The highly conserved members of the zic family of zinc-finger transcription factors are primarily known for their roles in embryonic signaling pathways and regulation of cellular proliferation and differentiation. This study describes sexual phenotype differences in abundances of zic2 mRNA in the preoptic area of the hypothalamus, a region strongly implicated in sexual behavior and function, in an adult teleost, Thalassoma bifasciatum. The bluehead wrasse (Thalassoma bifasciatum) is a valuable model for studying neuroendocrine processes because it displays two discrete male phenotypes, initial phase (IP) males and territorial, terminal phase (TP) males, and undergoes socially-controlled protogynous sex change. Previously generated microarray-based comparisons suggested that zic2 was upregulated in the brains of terminal phase males relative to initial phase males. To further explore this difference, we cloned a 727 bp sequence for neural zic2 from field-collected animals. Riboprobe-based in situ hybridization was employed to localize zic2 signal in adult bluehead brains and assess the relative abundance of brain zic2 mRNA across sexual phenotypes. We found zic2 mRNA expression was extremely abundant in the granular cells of the cerebellum and widespread in other brain regions including in the thalamus, hypothalamus, habenula, torus semicircularis, torus longitudinalis, medial longitudinal fascicle and telencephalic areas. Quantitative autoradiography and phosphorimaging showed zic2 mRNA hybridization signal in the preoptic area of the hypothalamus was significantly higher in terminal phase males relative to both initial phase males and females, and silver grain analysis confirmed this relationship between phenotypes. No significant difference in abundance was found in zic2 signal across phenotypes in the habenula, a brain region not implicated in the control of sexual behavior, or cerebellum

Expert range maps of global mammal distributions harmonised to three taxonomic authorities

AimComprehensive, global information on species' occurrences is an essential biodiversity variable and central to a range of applications in ecology, evolution, biogeography and conservation. Expert range maps often represent a species' only available distributional information and play an increasing role in conservation assessments and macroecology. We provide global range maps for the native ranges of all extant mammal species harmonised to the taxonomy of the Mammal Diversity Database (MDD) mobilised from two sources, the Handbook of the Mammals of the World (HMW) and the Illustrated Checklist of the Mammals of the World (CMW).LocationGlobal.TaxonAll extant mammal species.MethodsRange maps were digitally interpreted, georeferenced, error-checked and subsequently taxonomically aligned between the HMW (6253 species), the CMW (6431 species) and the MDD taxonomies (6362 species).ResultsRange maps can be evaluated and visualised in an online map browser at Map of Life (mol.org) and accessed for individual or batch download for non-commercial use.Main conclusionExpert maps of species' global distributions are limited in their spatial detail and temporal specificity, but form a useful basis for broad-scale characterizations and model-based integration with other data. We provide georeferenced range maps for the native ranges of all extant mammal species as shapefiles, with species-level metadata and source information packaged together in geodatabase format. Across the three taxonomic sources our maps entail, there are 1784 taxonomic name differences compared to the maps currently available on the IUCN Red List website. The expert maps provided here are harmonised to the MDD taxonomic authority and linked to a community of online tools that will enable transparent future updates and version control

SNAPSHOT USA 2019 : a coordinated national camera trap survey of the United States

This article is protected by copyright. All rights reserved.With the accelerating pace of global change, it is imperative that we obtain rapid inventories of the status and distribution of wildlife for ecological inferences and conservation planning. To address this challenge, we launched the SNAPSHOT USA project, a collaborative survey of terrestrial wildlife populations using camera traps across the United States. For our first annual survey, we compiled data across all 50 states during a 14-week period (17 August - 24 November of 2019). We sampled wildlife at 1509 camera trap sites from 110 camera trap arrays covering 12 different ecoregions across four development zones. This effort resulted in 166,036 unique detections of 83 species of mammals and 17 species of birds. All images were processed through the Smithsonian's eMammal camera trap data repository and included an expert review phase to ensure taxonomic accuracy of data, resulting in each picture being reviewed at least twice. The results represent a timely and standardized camera trap survey of the USA. All of the 2019 survey data are made available herein. We are currently repeating surveys in fall 2020, opening up the opportunity to other institutions and cooperators to expand coverage of all the urban-wild gradients and ecophysiographic regions of the country. Future data will be available as the database is updated at eMammal.si.edu/snapshot-usa, as well as future data paper submissions. These data will be useful for local and macroecological research including the examination of community assembly, effects of environmental and anthropogenic landscape variables, effects of fragmentation and extinction debt dynamics, as well as species-specific population dynamics and conservation action plans. There are no copyright restrictions; please cite this paper when using the data for publication.Publisher PDFPeer reviewe

Common Genetic Polymorphisms Influence Blood Biomarker Measurements in COPD

Implementing precision medicine for complex diseases such as chronic obstructive lung disease (COPD) will require extensive use of biomarkers and an in-depth understanding of how genetic, epigenetic, and environmental variations contribute to phenotypic diversity and disease progression. A meta-analysis from two large cohorts of current and former smokers with and without COPD [SPIROMICS (N = 750); COPDGene (N = 590)] was used to identify single nucleotide polymorphisms (SNPs) associated with measurement of 88 blood proteins (protein quantitative trait loci; pQTLs). PQTLs consistently replicated between the two cohorts. Features of pQTLs were compared to previously reported expression QTLs (eQTLs). Inference of causal relations of pQTL genotypes, biomarker measurements, and four clinical COPD phenotypes (airflow obstruction, emphysema, exacerbation history, and chronic bronchitis) were explored using conditional independence tests. We identified 527 highly significant (p 10% of measured variation in 13 protein biomarkers, with a single SNP (rs7041; p = 10−392) explaining 71%-75% of the measured variation in vitamin D binding protein (gene = GC). Some of these pQTLs [e.g., pQTLs for VDBP, sRAGE (gene = AGER), surfactant protein D (gene = SFTPD), and TNFRSF10C] have been previously associated with COPD phenotypes. Most pQTLs were local (cis), but distant (trans) pQTL SNPs in the ABO blood group locus were the top pQTL SNPs for five proteins. The inclusion of pQTL SNPs improved the clinical predictive value for the established association of sRAGE and emphysema, and the explanation of variance (R2) for emphysema improved from 0.3 to 0.4 when the pQTL SNP was included in the model along with clinical covariates. Causal modeling provided insight into specific pQTL-disease relationships for airflow obstruction and emphysema. In conclusion, given the frequency of highly significant local pQTLs, the large amount of variance potentially explained by pQTL, and the differences observed between pQTLs and eQTLs SNPs, we recommend that protein biomarker-disease association studies take into account the potential effect of common local SNPs and that pQTLs be integrated along with eQTLs to uncover disease mechanisms. Large-scale blood biomarker studies would also benefit from close attention to the ABO blood group

Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic

This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic