Numerical simulations of the flow and sediment transport regimes surrounding a short cylinder

Author Posting. © IEEE, 2007. This article is posted here by permission of IEEE for personal use, not for redistribution. The definitive version was published in IEEE Journal of Oceanic Engineering 32 (2007): 249-259, doi:10.1109/JOE.2007.890986.The 3-D flow field and bed stress surrounding a short cylinder in response to combined wave and mean-flow forcing events is examined. Model simulations are performed with a 3-D nonhydrostatic computational fluid dynamics model, FLOW-3D. The model is forced with a range of characteristic tidal and wave velocities as observed in 12–15 m of water at the Martha's Vineyard Coastal Observatory (MVCO, Edgartown, MA). The 2.4-m-long and 0.5-m diameter cylinder is buried 10% of the diameter on a flat, fixed bed. Regions of incipient motion are identified through local estimates of the Shields parameter exceeding the critical value. Potential areas of sediment deposition are identified with local estimates of the Rouse parameter exceeding ten. The model predictions of sediment response are in general in agreement with field observations of seabed morphology obtained over a one-week period during the 2003–2004 MVCO mine burial experiment. Both observations and simulations show potential transport occurring at the ends of the mine in wave-dominated events. Mean flows greater than 10 cm/s lead to the formation of larger scour pits upstream of the cylinder. Deposition in both cases tends to occur along the sides, near the center of mass of the mine. However, the fixed-bed assumption prohibits the prediction of full perimeter scour as is observed in nature. Predicted scour and burial regimes for a range of wave and mean-flow combinations are established.This work was supported in part by the U.S. Office of Naval Research under the mine burial project N00014-00-1-0570. The work of K. A. Hatton was supported by the National Science Foundation Graduate Research Fellowship

Silicon isotopes reveal a non-glacial source of silicon to Crescent Stream, McMurdo Dry Valleys, Antarctica

In high latitude environments, silicon is supplied to river waters by both glacial and nonglacial chemical weathering. The signal of these two end-members is often obscured by biological uptake and/or groundwater input in the river catchment. McMurdo Dry Valleys streams in Antarctica have no deep groundwater input, no connectivity between streams and no surface vegetation cover, and thus provide a simplified system for us to constrain the supply of dissolved silicon (DSi) to rivers from chemical weathering in a glacial environment. Here we report dissolved Si concentrations, germanium/silicon ratios (Ge/Si) and silicon isotope compositions (d30SiDSi) in Crescent Stream, McMurdo Dry Valleys for samples collected between December and February in the 20142015, 20152016, and 20162017 austral seasons. The d30SiDSi compositions and DSi concentrations are higher than values reported in wet-based glacial meltwaters, and form a narrow cluster within the range of values reported for permafrost dominated Arctic Rivers. High d30SiDSi compositions, ranging from C0.90h to C1.39h, are attributed to (i) the precipitation of amorphous silica during freezing of waters in isolated pockets of the hyporheic zone in the winter and the release of Si from unfrozen pockets during meltwater-hyporheic zone exchange in the austral summer, and (ii) additional Si isotope fractionation via long-term Si uptake in clay minerals and seasonal Si uptake into diatoms superimposed on this winter-derived isotope signal. There is no relationship between d30SiDSi compositions and DSi concentrations with seasonal and daily discharge, showing that stream waters contain DSi that is in equilibrium with the formation of secondary Si minerals in the hyporheic zone. We show that d30SiDSi compositions can be used as tracers of silicate weathering in the hyporheic zone and possible tracers of freeze-thaw conditions in the hyporheic zone. This is important in the context of the ongoing warming in McMurdo Dry Valleys and the supply of more meltwaters to the hyporheic zone of McMurdo Dry Valley streams

Literacy in Early Intervention for Children with Visual Impairments: Insights from Individual Cases.

A qualitative case study design was used to investigate the ways in which two early interventionists supported emergent literacy development for infants and toddlers with visual impairment. Three themes are addressed: (1) the importance of a family-centered approach in addressing emergent literacy in early intervention; (2) the role of the early interventionist in language and concept development; and (3) the need to focus on the senses as they relate to literacy. The findings provide practical insights into the role of the early interventionist in supporting early literacy development

Evolution from adherent to suspension: systems biology of HEK293 cell line development

The need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors. Despite the increased popularity of this strain we still have limited knowledge on the genetic composition of its derivatives. Here we present a genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. Changes in gene expression between adherent and suspension derivatives highlighted switching in cholesterol biosynthesis and expression of five key genes (RARG, ID1, ZIC1, LOX and DHRS3), a pattern validated in 63 human adherent or suspension cell lines of other origin

Enhanced metabolism and negative regulation of ER stress support higher erythropoietin production in HEK293 cells

Recombinant protein production can cause severe stress on cellular metabolism, resulting in limited titer and product quality. To investigate cellular and metabolic characteristics associated with these limitations, we compare HEK293 clones producing either erythropoietin (EPO) (secretory) or GFP (non-secretory) protein at different rates. Transcriptomic and functional analyses indicate significantly higher metabolism and oxidative phosphorylation in EPO producers compared with parental and GFP cells. In addition, ribosomal genes exhibit specific expression patterns depending on the recombinant protein and the production rate. In a clone displaying a dramatically increased EPO secretion, we detect higher gene expression related to negative regulation of endoplasmic reticulum (ER) stress, including upregulation of ATF6B, which aids EPO production in a subset of clones by overexpression or small interfering RNA (siRNA) knockdown. Our results offer potential target pathways and genes for further development of the secretory power in mammalian cell factories

Rehabilitation versus surgical reconstruction for non-acute anterior cruciate ligament injury (ACL SNNAP): a pragmatic randomised controlled trial

BackgroundAnterior cruciate ligament (ACL) rupture is a common debilitating injury that can cause instability of the knee. We aimed to investigate the best management strategy between reconstructive surgery and non-surgical treatment for patients with a non-acute ACL injury and persistent symptoms of instability.MethodsWe did a pragmatic, multicentre, superiority, randomised controlled trial in 29 secondary care National Health Service orthopaedic units in the UK. Patients with symptomatic knee problems (instability) consistent with an ACL injury were eligible. We excluded patients with meniscal pathology with characteristics that indicate immediate surgery. Patients were randomly assigned (1:1) by computer to either surgery (reconstruction) or rehabilitation (physiotherapy but with subsequent reconstruction permitted if instability persisted after treatment), stratified by site and baseline Knee Injury and Osteoarthritis Outcome Score—4 domain version (KOOS4). This management design represented normal practice. The primary outcome was KOOS4 at 18 months after randomisation. The principal analyses were intention-to-treat based, with KOOS4 results analysed using linear regression. This trial is registered with ISRCTN, ISRCTN10110685, and ClinicalTrials.gov, NCT02980367.FindingsBetween Feb 1, 2017, and April 12, 2020, we recruited 316 patients. 156 (49%) participants were randomly assigned to the surgical reconstruction group and 160 (51%) to the rehabilitation group. Mean KOOS4 at 18 months was 73·0 (SD 18·3) in the surgical group and 64·6 (21·6) in the rehabilitation group. The adjusted mean difference was 7·9 (95% CI 2·5–13·2; p=0·0053) in favour of surgical management. 65 (41%) of 160 patients allocated to rehabilitation underwent subsequent surgery according to protocol within 18 months. 43 (28%) of 156 patients allocated to surgery did not receive their allocated treatment. We found no differences between groups in the proportion of intervention-related complications.InterpretationSurgical reconstruction as a management strategy for patients with non-acute ACL injury with persistent symptoms of instability was clinically superior and more cost-effective in comparison with rehabilitation management

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Correction to: Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

The original version of this article unfortunately contained a mistake