Hatton, Diane

Lang, Steven

English

Engineering Conferences International

Engineering Conferences InternationalECI Digital ArchivesCell Culture Engineering XVI Proceedings5-8-2018Advances in Cell Line Engineering and ProteinExpression StrategiesSteven LangGenentechDiane HattonMedImmuneFollow this and additional works at: http://dc.engconfintl.org/ccexviPart of the Engineering CommonsThis Article is brought to you for free and open access by the Proceedings at ECI Digital Archives. It has been accepted for inclusion in Cell CultureEngineering XVI by an authorized administrator of ECI Digital Archives. For more information, please contact franco@bepress.com.Recommended CitationSteven Lang and Diane Hatton, "Advances in Cell Line Engineering and Protein Expression Strategies" in "Cell Culture EngineeringXVI", A. Robinson, PhD, Tulane University R. Venkat, PhD, MedImmune E. Schaefer, ScD, J&J Janssen Eds, ECI Symposium Series,(2018). http://dc.engconfintl.org/ccexvi/231Advances in Cell Line Engineering and Protein Expression StrategiesScope: Despite the wide use of CHO cells it has been a challenge to optimize production of biologics beyond screening and upstream process improvements. This paradigm is changing with the maturation of “omics” and gene editing techniques. The industry is experiencing a renewed interest in pioneering efforts to better understand CHO cells and engineer drug manufacturing productivity and quality. This workshop will focus on sharing the latest concepts in automation in cell line development, engineering for product quality and process performance as well as the strategies for expressing novel protein formats. The discussion will span many topics including,• Current state of CHO genome sequences• Genome editing technologies• Engineered CHO cell lines, including contaminant-free CHO cell lines• Metabolism maps• Targeted integrationDiane Hatton (MedImmune) and Steve Lang (Genentech)Advances in Cell Line Engineering and Protein Expression Strategies• Agenda:– 20min opener and survey results– 25min table discussion on selected topics– 45min table read out and open discussion• Students to help in discussions, taking notes• Philosophy:– Work as table groups on different topics– Opportunity to interact with new people– Seek out different expertise and perspectives– Have plenty of time for discussion– Share outputsTopics for tables1. Host cell line engineering 2. "omics" technologies3. Transgene ‘mutation’ screening4. CLD for continuous processing5. Synthetic biology CLD strategies6. Clonality for legacy cell lines7. Targeted/inducible expression systems8. Automation & data management9. New formats and non-traditional therapies10. Timeline acceleration11. CHO alternatives and cell-free systemsPotential discussion points to be distributed to tables.Questions to discuss and read out• Each table is listed with a topic and potential discussion points.• Please select a table/topic that you would like to discuss.• Discuss topics and prepare a synopsis for sharing with the entire group. • Additional questions if you run out:– What other pain points are you experiencing?– What are we missing in the topic list?• Suggestions for each table– Roundtable introductions– Define roles• Scribe, Timekeeper, PresenterPlease rank the 3 topics of greatest interest to you0% 10% 20% 30% 40% 50%Host cell line engineering"omics" technologiesCLD for continuous processingTargeted/Inducible expression systemsSynthetic biology CLD strategiesAutomation and data managementNew formats and non-traditional therapiesClonality for legacy cell linesTransgene ‘mutation’ screeningPercentage of RespondantsRank 1Rank 2Rank 3Q30Interest score aggregate Most common 1st topicMost common 2nd topicMost common 3rd topic** Most respondents selected more than 3 topicsWhich of the above topics are you working on?Q310% 10% 20% 30% 40% 50% 60%Host cell line engineering"omics" technologiesAutomation and data managementTargeted/Inducible expressionCLD for continuous processingClonality for legacy cell linesNew formats and non-traditional therapiesSynthetic biology CLD strategiesTransgene ‘mutation’ screeningPercentage of Respondants** Most respondents selected more than 3 topicsIn the next 5 years, what do you think will be the biggest challenge for stable cell line development?Q32In the next 5 years, what could be disruptive technologies for cell line and process development?Q33Teaser• Share your challenges and learn from others about advances in cell line engineering and expression strategies. Win a prize for correctly guessing the greatest challenge to cell line development and the most anticipated disruptive technology!

Advances in Cell Line Engineering and Protein Expression Strategies

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Advances in Cell Line Engineering and Protein Expression Strategies

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