Conservation agriculture has no significant impact on sheep digestive parasitism

Conservation agriculture (CONS A) is a sustainable agriculture system based on crop rotation with no tillage. It has various environmental advantages compared to conventional agriculture (CONV A): decreased water evaporation, erosion, and CO2 emissions. In this first study of its kind, we aim to evaluate the impact of this type of agriculture on sheep gastrointestinal parasites. Two lamb groups aged between 5 and 10 months were randomly included to graze separately on CONS A and CONV A pastures. Each group was composed of two batches of three lambs, and these were followed up for two rearing months. Liveweight, hematological parameter variation, and digestive parasites were studied. At the end of the study period, lambs were slaughtered the carcass yield was determined, and a helminthological autopsy was performed on the digestive tracts of the animals to estimate different parasitological indicators. There was no difference between lambs reared on CONS A and those reared on CONV A for all parasite indicators (infestation intensity, abundance, and prevalence). The same trend was also obtained for hematological parameters, liveweight evolution, and carcass yield. These results prove that there is no impact of CONS A on the sheep's digestive parasitism. Further studies are needed to support these findings on larger animal samples and to investigate the impact of conservation agriculture on other parasite species. Similar studies could also be conducted on ruminant species

Assessing complementary synergies for integrated crop–livestock systems under conservation agriculture in Tunisian dryland farming systems

The aim of this paper is twofold. The first objective is to measure the technical efficiency of mixed crop-livestock (CL) smallholder producers operating under conservation agriculture systems in Tunisian rainfed areas. The second objective is to explore complementarities, synergies, and economies of diversification across the different production system components of these crop-livestock producers using the cross-partial derivative framework of output variables in the distance function. A simple random sampling process was employed to select and survey 59 CL smallholders operating under conservation agriculture. The collected data were analyzed using a stochastic input distance function in which synergies were estimated based on the second cross-partial derivative concept of output variables in the distance function. Results show that technical inefficiencies are significant in integrated crop-livestock systems, and there is evidence that economic diversification provides a productivity buffer against climate change threats. As a sustainable intensification strategy, this integrated system also offers a potential advantage. The results further contribute to the debate on crop diversification vs. specialization. Although an enhanced system integration could be a financially and ecologically viable option for mixed crop-livestock systems, more pathways for profitable and viable diversification of cereal-based or orchard-based systems remain to be explored

Refinement and Scaling of Inclusive Agroecological Innovations for Livestock Management, Crop Rotations, and Soil Conservation in Semi-arid South Mediterranean Regions

The objective of this work is to i) present a socio-technical package of agroecological interventions that are already being tested for this type of mixed farming system in the semi-arid area of Tunisia, and ii) illustrate the scope and mechanisms for scaling up this package. Results further illustrate the key factors that have led to a change in attitude and behaviour among local actors and farmers towards a better co-generation, co-sharing, and adoption of agroecological principles at farm, community, and landscape levels. This work was presented at the Tropentag 2022 conference in Prague Czech University of Life Sciences, Prague/Czech Republic: September 14-1

Purification and biochemical characterization of a novel thermostable protease from the oyster mushroom Pleurotus sajor-caju strain CTM10057 with industrial interest

Background Proteases are hydrolytic enzymes that catalyze peptide linkage cleavage reactions at the level of proteins and peptides with different degrees of specificity. This group draws the attention of industry. More than one protease in three is a serine protease. Classically, they are active at neutral to alkaline pH. The serine proteases are researched for industrial uses, especially detergents. They are the most commercially available enzyme group in the world market. Overall, fungi produced extracellular proteases, easily separated from mycelium by filtration. Results A new basidiomycete fungus CTM10057, a hyperproducer of a novel protease (10,500U/mL), was identified as Pleurotus sajor-caju (oyster mushroom). The enzyme, called SPPS, was purified to homogeneity by heat-treatment (80 C for 20min) followed by ammonium sulfate precipitation (35-55%)-dialysis, then UNO Q-6 FPLC ion-exchange chromatography and finally HPLC-ZORBAX PSM 300 HPSEC gel filtration chromatography, and submitted to biochemical characterization assays. The molecular mass was estimated to be 65 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Native-PAGE, casein-zymography, and size exclusion by HPLC. A high homology with mushroom proteases was displayed by the first 26 amino-acid residues of the NH2-terminal aminoacid sequence. Phenylmethanesulfonyl fluoride (PMSF) and diiodopropyl fluorophosphates (DFP) strongly inhibit SPPS, revealing that it is a member of the serine-proteases family. The pH and temperature optima were 9.5 and 70 C, respectively. Interestingly, SPPS possesses the most elevated hydrolysis level and catalytic efficiency in comparison with SPTC, Flavourzyme 500 L, and Thermolysin type X proteases. More remarkably, a high tolerance towards organic solvent tolerance was exhibited by SPPS, together with considerable detergent stability compared to the commercial proteases Thermolysin type X and Flavourzyme 500 L, respectively. Conclusions This proves the excellent proprieties characterizing SPPS, making it a potential candidate for industrial applications especially detergent formulations

Context Assessment for Agroecology Transformation in the Tunisian Living Landscape

The purpose of this Context Assessment is threefold: first, to characterize the environmental, social and economic and political contexts of the Tunisian ALL; second, to understand the data and information currently available in sub-region of the ALL, and third to characterize the extent to which agroecological principles are already being employed locally at the ALL levels. This report constitutes a basis of information and discussion to conduct the impact assessment. It is also valuable to all WPs in the Initiative as it provides critical quantitative or qualitative data and information regarding capacities assessment, policy influence, and other environmental attributes which can guide the initiative implementation and impact in 2023/2024. The present Context Assessment in Tunisia has been elaborated from primary and secondary sources of data. The primary sources of data are issued from focus groups and formal and informal interviews conducted in the targeted area between June and December 2022, as part of WP1 and WP4 activities. The secondary sources of data came from previous research and development projects, in addition to formal and grey literature or technical reports and policy documents. This report will be enriched with a household survey planned during the first quarter of 2023. This report contributes to Output 2.1. Baseline – current conditions of agricultural systems of small holder farmers in each ALL, Output 1.1 on establishment of the ALL, Output 4.1 on the identification of policies and local institutions and their role in the AE pathways

Visioning workshops in the Tunisian agroecologial living landscapes (ALL): Preliminary Data Sets of the Visioning Approach

Within the “Transdisciplinary co-creation of innovations in Agroecological Living Landscape-ALL” Work Package (WP) of the Transformational Agroecology Across Food, Land and Water Systems Initiative (AE-I) , it is proposed to use/apply participatory methods to build collectively a vision of the desired agroecological pathways in each ALL. This activity aims at developing the most suitable agroecological transition pathways in each ALL identified and agreed upon among actors. The Visioning activity was collectively conducted by ICARDA, and its national partners involved in the AE-I under the supervision with all the WP leaders in Tunisian and the support of the “Transdisciplinary co-creation of innovations in Agroecological Living Landscapes” work package WP1 and the “Strengthening the policy- and institutional-enabling environment” work package (WP5) leaders and co-leaders in the first phase of the implementation. This technical report restitutes the primary data collected during the Visioning approach that will support further analysis in the coming months to support the next steps of codesigning and co implementing the technical and organizational agro-ecological innovative packages with the stakeholders in the Tunisian ALL

Pulmonary embolism in intensive care unit: Predictive factors, clinical manifestations and outcome

<b>Objective</b> :<b> </b> To determine predictive factors, clinical and demographics characteristics of patients with pulmonary embolism (PE) in ICU, and to identify factors associated with poor outcome in the hospital and in the ICU. <b> Methods</b> :<b> </b> During a four-year prospective study, a medical committee of six ICU physicians prospectively examined all available data for each patient in order to classify patients according to the level of clinical suspicion of pulmonary thromboembolism. During the study periods, all patients admitted to our ICU were classified into four groups. The first group includes all patients with confirmed PE; the second group includes some patients without clinical manifestations of PE; the third group includes patients with suspected and not confirmed PE and the fourth group includes all patients with only deep vein thromboses (DVTs) without suspicion of PE. The diagnosis of PE was confirmed either by a high-probability ventilation/perfusion (V/Q) scan or by a spiral computed tomography (CT) scan showing one or more filling defects in the pulmonary artery or in its branches. The diagnosis was also confirmed by echocardiography when a thrombus in the pulmonary artery was observed. <b> Results</b> :<b> </b> During the study periods, 4408 patients were admitted in our ICU. The diagnosis of PE was confirmed in 87 patients (1.9&#x0025;). The mean delay of development of PE was 7.8 &#177; 9.5 days.<b> </b> On the day of PE diagnosis, clinical examination showed that 50 patients (57.5&#x0025;) were hypotensive, 63 (72.4&#x0025;) have SIRS, 15 (17.2&#x0025;) have clinical manifestations of DVT and 71 (81.6&#x0025;) have respiratory distress requiring mechanical ventilation. In our study, intravenous unfractionated heparin was used in 81 cases (93.1&#x0025;) and low molecular weight heparins were used in 4 cases (4.6&#x0025;).<b> </b> The mean ICU stay was 20.2 &#177; 25.3 days and the mean hospital stay was 25.5 &#177; 25 days. The mortality rate in ICU was 47.1&#x0025; and the in-hospital mortality rate was 52.9&#x0025;.<b> </b> Multivariate analysis showed that factors associated with a poor prognosis in ICU are the use of norepinephrine and epinephrine<b> . </b> Furthermore, factors associated with in-hospital poor outcome in multivariate analysis were a number of organ failure associated with PE &#8805; 3. Moreover, comparison between patients with and without pe showed that predictive factors of pe are: acute medical illness, the presence of meningeal hemorrhage, the presence of spine fracture, hypoxemia with PaO₂ /FiO₂ ratio &#60; 300 and the absence of pharmacological prevention of venous thromboembolism. <b>Conclusion</b> :<b> </b> Despite the high frequency of DVT in critically ill patients, symptomatic PE remains not frequently observed, because systematic screening is not performed. Pulmonary embolism is associated with a high ICU and in-hospital mortality rate. Predictive factors of PE are acute medical illness, the presence of meningeal hemorrhage, the presence of spine fracture, hypoxemia with PaO₂ /FiO₂ &lt; 300 and the absence of pharmacological prevention of venous thromboembolism

Isolated traumatic head injury in children: Analysis of 276 observations

<b>Background</b> : To determine predictive factors of mortality among children after isolated traumatic brain injury. <b>Materials and Methods</b> : In this retrospective study, we included all consecutive children with isolated traumatic brain injury admitted to the 22-bed intensive care unit (ICU) of Habib Bourguiba University Hospital (Sfax, Tunisia). Basic demographic, clinical, biochemical, and radiological data were recorded on admission and during ICU stay. <b>Results</b> : There were 276 patients with 196 boys (71&#x0025;) and 80 girls, with a mean age of 6.7 &#177; 3.8 years. The main cause of trauma was road traffic accident (58.3&#x0025;). Mean Glasgow Coma Scale score was 8 &#177; 2, Mean Injury Severity Score (ISS) was 23.3 &#177; 5.9, Mean Pediatric Trauma Score (PTS) was 4.8 &#177; 2.3, and Mean Pediatric Risk of Mortality (PRISM) was 10.8 &#177; 8. A total of 259 children required mechanical ventilation. Forty-eight children (17.4&#x0025;) died. Multivariate analysis showed that factors associated with a poor prognosis were PRISM &gt; 24 (OR: 10.98), neurovegetative disorder (OR: 7.1), meningeal hemorrhage (OR: 2.74), and lesion type VI according to Marshall tomographic grading (OR: 13.26). <b>Conclusion</b> : In Tunisia, head injury is a frequent cause of hospital admission and is most often due to road traffic injuries. Short-term prognosis is influenced by demographic, clinical, radiological, and biochemical factors. The need to put preventive measures in place is underscored