Regulated motion of glycoproteins revealed by direct visualization of a single cargo in the endoplasmic reticulum

The quality of cargo proteins in the endoplasmic reticulum (ER) is affected by their motion during folding. To understand how the diffusion of secretory cargo proteins is regulated in the ER, we directly analyze the motion of a single cargo molecule using fluorescence imaging/fluctuation analyses. We find that the addition of two N-glycans onto the cargo dramatically alters their diffusion by transient binding to membrane components that are confined by hyperosmolarity. Via simultaneous observation of a single cargo and ER exit sites (ERESs), we could exclude ERESs as the binding sites. Remarkably, actin cytoskeleton was required for the transient binding. These results provide a molecular basis for hypertonicity-induced immobilization of cargo, which is dependent on glycosylation at multiple sites but not the completion of proper folding. We propose that diffusion of secretory glycoproteins in the ER lumen is controlled from the cytoplasm to reduce the chances of aggregation

Research and development of a laparoscopic surgical device for ligating endless organs based on a flexible structure

While laparoscopic surgery has become increasingly widely used, many laparoscopic procedures are time-consuming and difficult to accomplish compared to open surgery. One such procedure is the ligation of endless organs. In this paper, the development and prototyping of a laparoscopic instrument that could significantly increase the efficiency of laparoscopic ligation is outlined. The mechanism is based on a snake-like flexible structure which is actuated by control wires. A simple simulation was carried out by both experienced surgical staff as well as non-surgical persons to confirm the effectiveness of the proposed mechanism

Expression of Smooth Muscle Calponin in Synovial Sarcoma

Purpose. Histogenesis of synovial sarcoma remains controversial and reliable molecular markers for diagnosis are necessary. Expression of basic calponin, a smooth muscle differentiation-specific actin-binding protein, was studied in synovial sarcoma

高校教育の量的変動と進路分化(Ⅲ) : 階層構造の視点から

日本教育社会学会第36回大会, 1984年10月(静岡大学), Ⅱ-4部会 学校の社会学(Ⅱ

高校教育の量的変動と進路分化(Ⅱ) : 府県の社会経済条件の視点から

日本教育社会学会第35回大会, 1983年10月(武蔵大学), 一般研究 Ⅲ-6部会 教育機