miR-608 rs4919510 C>G polymorphism decreased the risk of breast cancer in an Iranian subpopulation

Aim: MicroRNAs (miRNAs) are small noncoding RNAs that function as oncogene or tumor suppressors. The single nucleotide polymorphisms in miRNAs potentially can alter miRNA-binding sites on target genes as well as affecting miRNAs expression. The present study aimed to evaluate the impact of miR-608 rs4919510 C>G variant on breast cancer (BC) risk. Materials and Methods: This case-control study conducted on 160 women with BC and 192 age-matched healthy women. Genotyping of miR608 rs4919510 was done using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: Our findings showed that GC genotype significantly decreased the risk of BC (odds ratio (OR) = 0.49, 95% confidence interval (CI) 0.28–0.88, p = 0.018) compared to CC genotype. Furthermore the G allele decreased the risk of BC (OR = 0.53, 95%CI 0.30–0.92, p = 0.024). No significant association was found between miR-609 genotypes and clinicopathological characteristics of BC patients (p > 0.05). Conclusion: Our findings indicate that miR-608 polymorphism might be associated with decreased risk of BC in an Iranian subpopulation. Further large-scale studies with different ethnicities are needed to verify our findings

SLIM at LHC: LHC search power for a model linking dark matter and neutrino mass

Recently a model has been proposed that links dark matter and neutrino masses. The dark matter candidate which is dubbed as SLIM has a mass of MeV scale and can show up at low energy experiments. The model also has a high energy sector which consists of a scalar doublet, $(\phi^-, \phi^0)$. We discuss the potential of the LHC for discovering the new scalars. We focus on the $\phi^+\phi^-$ and $\phi^{\pm} \phi^0$ production and the subsequent decay of the charged scalar to a charged lepton and the SLIM which appears as missing energy. Identifying the background, we estimate the signal significance and find that it can exceed $5 \sigma$ at 30 ${\rm fb}^{-1}$ for the 14 TeV run at the LHC. We comment on the possibility of extracting the flavor structure of the Yukawa couplings which also determine the neutrino mass matrix. Finally, we discuss the prospects of this search at the current 7 TeV run of the LHC.Comment: 26 pages, 21 figure

Intravenous Paracetamol Versus Patient-Controlled Analgesia With Morphine for the Pain Management Following Diagnostic Knee Arthroscopy in Trauma Patients: A Randomized Clinical Trial

Background: Most patients undergoing outpatient surgeries have the unpleasant experience of high level pain after surgery. Compared with open surgeries, arthroscopic procedures are less painful; however, inadequate pain management could be associated with significant concerns. Opioids alone or in combination with local anesthetics are frequently used for diminishing postoperative pain using intravenous or epidural infusion pumps. Despite morphine various disadvantages, it is commonly used for controlling pain after surgery. Objectives: The aim of this study was to compare intravenous paracetamol and patient-controlled analgesia (PCA) with morphine for the pain management following diagnostic knee arthroscopy in trauma patients. Patients and Methods: Sixty trauma patients who were scheduled to undergo knee arthroscopy were randomly divided into two groups. Patients immediately received intravenous infusion of 1 g paracetamol within 15 minutes after surgery and every 6 hours to 24 hours in the paracetamol group. The patient-controlled analgesia group received morphine through PCA infusion pump at 2 mL/h base rate and 1mL bolus every 15 minutes. Pain level, nausea and vomiting, and sedation were measured and recorded during entering the recovery, 15 and 30 minutes after entering the recovery, 2, 6, and 24 hours after starting morphine pump infusion in the morphine and paracetamol in the paracetamol groups. Results: There was no significant difference regarding the pain level at different times after entering the recovery between the two groups. No one from the paracetamol group developed drug complications. However, 22.3% in the PCA morphine suffered from postoperative nausea; there was a statistically significant difference regarding the sedation level, nausea, and vomiting at various times between the two groups. Conclusions: Intravenous administration of paracetamol immediately after knee arthroscopy improved postoperative pain, decreased analgesic administration, maintained stable hemodynamic parameters, had no complications related to opiates, no nausea and vomiting, and increased patient satisfaction and comfort in comparison to PCA with morphine

Evaluation and comparison of SYBR Green I Real-Time PCR and TaqMan Real-Time PCR methods for quantitative assay of Listeria monocytogenes in nutrient broth and milk

Specific traditional plate count method and real-time PCR systems based on SYBR Green I and TaqMan technologies using a specific primer pair and probe for amplification of iap-gene were used for quantitative assay of Listeria monocytogenes in seven decimal serial dilution series of nutrient broth and milk samples containing 1.58 to 1.58×107 cfu /ml and the real-time PCR methods were compared with the plate count method with respect to accuracy and sensitivity. In this study, the plate count method was performed using surface-plating of 0.1 ml of each sample on Palcam Agar. The lowest detectable level for this method was 1.58×10 cfu/ml for both nutrient broth and milk samples. Using purified DNA as a template for generation of standard curves, as few as four copies of the iap-gene could be detected per reaction with both real-time PCR assays, indicating that they were highly sensitive. When these real-time PCR assays were applied to quantification of L. monocytogenes in decimal serial dilution series of nutrient broth and milk samples, 3.16×10 to 3.16×105 copies per reaction (equals to 1.58×103 to 1.58×107 cfu/ml L. monocytogenes) were detectable. As logarithmic cycles, for Plate Count and both molecular assays, the quantitative results of the detectable steps were similar to the inoculation levels

Tidal stream resource assessment uncertainty due to flow asymmetry and turbine yaw misalignment

The majority of tidal energy convertors (TECs) currently under development are of a non-yawing horizontal axis design. However, most energetic regions that have been identified as candidate sites for installation of TEC arrays exhibit some degree of directional and magnitude asymmetry between incident flood and ebb flow angles and velocities, particularly in nearshore environments where topographic, bathymetric and seabed frictional effects and interactions are significant. Understanding the contribution of directional and magnitude asymmetry to resource power density along with off axis rotor alignment to flow could influence site selection and help elucidate optimal turbine orientation. Here, 2D oceanographic model simulations and field data were analysed to investigate these effects at potential deployment locations in the Irish Sea; an energetic semi-enclosed shelf sea region. We find that observed sites exhibiting a high degree of asymmetry may be associated with a reduction of over 2% in annual energy yield when deployment design optimisation is ignored. However, at the majority of sites, even in the presence of significant asymmetry, the difference is \u3c0.3%. Although the effects are shown to have less significance than other uncertainties in resource assessment, these impacts could be further investigated and quantified using CFD and 3D modelling

Charged Higgs Observability Through Associated Production With W at a Muon Collider

The observability of a charged Higgs boson produced in association with a W boson at future muon colliders is studied. The analysis is performed within the MSSM framework. The charged Higgs is assumed to decay to tb and a fully hadronic final state is analyzed, i.e., mu+mu- \rightarrow H\pmW\mp \rightarrow tbW \rightarrow WbbW \rightarrow jjjjbb. The main background is tt production in fully hadronic final state which is an irreducible background with very similar kinematic features. It is shown that although the discovery potential is almost the same for a charged Higgs mass in the range 200 GeV < mH\pm < 400 GeV, the signal significance is about 1sigma for tanbeta = 50 at integrated luminosity of 50 fb-1. The signal rate is well above that at e+e- linear colliders with the same center of mass energy and enough data (O(1 ab-1)) will provide the same discovery potential for all heavy charged Higgs masses up to mH\pm \sim 400 GeV, however, the muon collider cannot add anything to the LHC findings.Comment: 18 pages, 11 figure

Cisplatin Induces Up-Regulation of KAI1, a Metastasis Suppressor Gene, in MCF-7 Breast Cancer Cell Line

Purpose: To investigate the effect of cisplatin on cell toxicity and metastasis through modulation of KAI1 gene expression.Methods: MCF-7cells were incubated with different concentrations of cisplatin for 24 h. RNA was extracted by trizol and cDNA synthesized. KAI1 and TBP were chosen as target and internal control genes, respectively. Specific primers were designed by primer express software, v.3.0. KAI1/TBP and gene expression ratio was calculated using the formula, 2 -&#916;&#916;Ct.Results: Cisplatin exerted a dose-dependent inhibitory effect on the viability of highly metastatic MCF-7 cells. KAI1/TBP gene expression ratios were 1.97 &#177; 0.19 (p &lt; 0.05), 2.96 &#177; 0.55 (p &lt; 0.05), 9.06 &#177; 0.27 (p &lt; 0.001) and 12.38 &#177; 0.88 (p &lt; 0.01) in 10, 20, 50 and 100 &#956;M concentrations of cisplatin. Conclusion: These findings indicate that cisplatin can inhibit metastasis by up-regulating KAI1 gene in MCF-7cells.Keywords: Cisplatin; KAI1; Metastasis; Breast Cancer; Real-time PCR

Right-Handed Sector Leptogenesis

Instead of creating the observed baryon asymmetry of the universe by the decay of right-handed (RH) neutrinos to left-handed leptons, we propose to generate it dominantly by the decay of the RH neutrinos to RH leptons. This mechanism turns out to be successful in large regions of parameter space. It may work, in particular, at a scale as low as $\sim$~TeV, with no need to invoke quasi-degenerate RH neutrino masses to resonantly enhance the asymmetry. Such a possibility can be probed experimentally by the observation at colliders of a singlet charged Higgs particle and of RH neutrinos. Other mechanisms which may lead to successful leptogenesis from the RH lepton sector interactions are also briefly presented. The incorporation of these scenarios in left-right symmetric and unified models is discussed.Comment: 14 pages, latex, axodraw; minor clarifications and references added, extended discussion of the signatures at collider

The potential use of service-oriented infrastructure framework to enable transparent vertical scalability of cloud computing infrastructure

Cloud computing technology has become familiar to most Internet users. Subsequently, there has been an increased growth in the use of cloud computing, including Infrastructure as a Service (IaaS). To ensure that IaaS can easily meet the growing demand, IaaS providers usually increase the capacity of their facilities in a vertical IaaS increase capability and the capacity for local IaaS amenities such as increasing the number of servers, storage and network bandwidth. However, at the same time, horizontal scalability is sometimes not enough and requires additional strategies to ensure that the large number of IaaS service requests can be met. Therefore, strategies requiring horizontal scalability are more complex than the vertical scalability strategies because they involve the interaction of more than one facility at different service centers. To reduce the complexity of the implementation of the horizontal scalability of the IaaS infrastructures, the use of a technology service oriented infrastructure is recommended to ensure that the interaction between two or more different service centers can be done more simply and easily even though it is likely to involve a wide range of communication technologies and different cloud computing management. This is because the service oriented infrastructure acts as a middle man that translates and processes interactions and protocols of different cloud computing infrastructures without the modification of the complex to ensure horizontal scalability can be run easily and smoothly. This paper presents the potential of using a service-oriented infrastructure framework to enable transparent vertical scalability of cloud computing infrastructures by adapting three projects in this research: SLA@SOI consortium, Open Cloud Computing Interface (OCCI), and OpenStack