Gay and Lesbian Candidate Electability

There is a sizable literature researching how individual's demographics (gender, age, race/ethnicity, religiosity, and political ideology) affect their opinions toward gays and lesbians, homosexuality, and LGBT rights. However, there is little to no literature regarding how these variables affect whether an individual would vote for a gay or lesbian candidate for elected office (candidate electability). That is what this research sets out to do in addition to determining how gay and lesbian candidate electability compares to that of their straight counterparts. To accomplish this an experimental design was used where each respondent was given two hypothetical candidate biographies with one being the control candidate (straight man) and the other being one of four randomly assigned candidates (straight man, straight woman, gay, or lesbian). The respondents were then asked to choose which candidate they preferred to vote for and how strongly they felt about their decision. This information was compiled to create an electability scale for each of the four types of candidates. Respondents were also asked about their demographic information. The results indicate that gay and lesbian candidates have lower electability than their straight counterparts with gay candidates having slightly higher electability than lesbian candidates. A person's religiosity (how religious they are) has a significant negative relationship with both gay and lesbian candidate electability. There is also evidence that the more conservative an individual is the less likely they would be to vote for both a gay or a lesbian candidate. At minimum, some support was found for all of the hypotheses concerning respondent demographic variables and their effects on gay candidate electability. Less consistent results were found concerning lesbian candidate electability.Political Scienc

Nursing Students’ Perceptions of How Working as Nursing Assistants Impacts Their Education

The purpose of this phenomenological study was to develop an understanding of how working as a nursing assistant impacts the ability of nursing students to meet the high demands of school during their junior year of a Baccalaureate of Science in Nursing (BSN) pre-licensure program. Benner’s Novice to Expert Theory, Situated Learning Theory, and the Johari Window Communication Model each suggested that immersing oneself in the culture of nursing would have positive outcomes for the student working as a nursing assistant. A qualitative phenomenological design was used to explore the following research questions: (a) What are the unique experiences of junior nursing students who have worked or are working as nursing assistants while in school? (b) How do nursing students who work as nursing assistants perceive that their work impacts their schoolwork? and (c) What is the mean academic achievement on junior level core nursing courses of students who work as nursing assistants in comparison to other nursing students in the same courses who worked in non-nursing jobs? Individual interviews, a focus group, and mean nursing grade comparisons were used to explore these questions among a purposeful sample of seven senior level nursing students in a baccalaureate nursing program in central Virginia who had worked as nursing assistants. Findings suggested that nursing faculty should encourage students to work as nursing assistants as long as they work less than 18 hours per week and focus on learning from nursing personnel while developing their skills as a nursing assistant

Dactinomycin Impairs Cellular Respiration and Reduces Accompanying ATP Formation

The effect of dactinomycin on cellular respiration and accompanying ATP formation was investigated in Jurkat and HL-60 cells. Cellular mitochondrial oxygen consumption (measured by a homemade phosphorescence analyzer) and ATP content (measured by the luciferin-luciferase bioluminescence system) were determined as functions of time t during continuous exposure to the drug. The rate of respiration, k, was the negative of the slope of [O2] versus t. Oxygen consumption and ATP content were diminished by cyanide, confirming that both processes involved oxidations in the mitochondrial respiratory chain. In the presence of dactinomycin, k decreased gradually with t, the decrease being more pronounced at higher drug concentrations. Cellular ATP remained constant for 5 h in untreated cells, but in the presence of 20 microM dactinomycin it decreased gradually (to one-tenth the value at 5 h for untreated cells). The drug-induced inhibition of respiration and decrease in ATP were blocked by the pancaspase inhibitor benzyloxycarbonyl-Val-Ala-DL-Asp-fluoromethyl ketone (zVAD-fmk). A rapid but temporary decrease in cellular ATP observed on the addition of zVAD-fmk was shown to be due to DMSO (added with zVAD-fmk). The effect of dactinomycin on respiration differed from that of doxorubicin. Plots of [O2] versus t were curved for dactinomycin so that k decreased gradually with t. The corresponding plots for doxorubicin were well fit by two straight lines; so k was constant for approximately 150 min, at which time k decreased, remaining constant at a lower level thereafter. The results for cells treated with mixtures of the two drugs indicated that the drugs acted synergistically. These results show the onset and severity of mitochondrial dysfunction in cells undergoing apoptosis induced by dactinomycin

Effects of Cisplatin on Mitochondrial Function in Jurkat Cells

In this work, we measured the effects of pharmacological concentrations of cisplatin (cis-diaminedichloroplatinum II) on mitochondrial function, cell viability, and DNA fragmentation in Jurkat cells. The exposure of cells to 0-25 microM cisplatin for 3 h had no immediate effect on cellular mitochondrial oxygen consumption, measured using a palladium-porphyrin oxygen sensing phosphor. Similarly, the cell viability as measured by trypan blue staining was unchanged immediately following exposure to the drug, and no small DNA fragments, characteristic of drug-induced apoptosis, appeared. At 24 h after exposure to cisplatin, cellular respiration and viability decreased relative to controls and the amount of small DNA fragments, measured using quantitative agarose gel electrophoresis, was proportional to the concentration of cisplatin present during the drug exposure period. The small DNA fragments showed the banding pattern (with a spacing of approximately 300 bp) characteristic of drug-induced cell death by apoptosis. The changes in respiration and DNA fragmentation correlated linearly with the amount of platinum bound to DNA, determined by atomic absorption spectroscopy immediately following drug exposure. The oxygen consumption by beef heart mitochondria was not affected 0-24 h after exposure to 25 microM cisplatin or to solutions containing the monoaquated form of the drug, suggesting that the drug does not attack the mitochondrial respiratory chain directly. Cells exposed to the peptide benzyloxycarbonyl-val-ala-asp-fluoromethyl ketone, which blocks apoptosis by the caspase pathway, showed a decrease in cisplatin-induced DNA fragmentation but not in the impairment of cellular respiration. Thus, although apoptosis is caspase-dependent, the impairment of cellular respiration is independent of the caspase system. Collectively, these results suggest that alteration in mitochondrial function is a secondary effect of cisplatin cytotoxicity in Jurkat cells

Inhibition of Cellular Respiration by Doxorubicin

Doxorubicin executes apoptosis, a process known to produce leakage of cytochrome c and opening of the mitochondrial permeability transition pores. To define the loss of mitochondrial function by apoptosis, we monitored cellular respiration during continuous exposure to doxorubicin. A phosphorescence analyzer capable of stable measurements over at least 5 h was used to measure [O(2)]. In solutions containing glucose and cells, [O(2)] declined linearly with time, showing that the kinetics of oxygen consumption was zero order. Complete inhibition of oxygen consumption by cyanide indicated that oxidations occurred in the respiratory chain. A decline in the rate of respiration was evident in Jurkat and HL-60 cells exposed to doxorubicin. The decline was abrupt, occurring after about 2 h of incubation. The inhibition was concentration-dependent and was completely blocked by the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone. Respiration in resistant HL-60/MX2 cells, characterized by an altered topoisomerase II activity, was not inhibited by doxorubicin. A decline in cellular ATP was measured in Jurkat cells after 2-4 h of incubation with 20 microM doxorubicin, paralleling the decline in respiration rate. Thus, cells incubated with doxorubicin exhibit caspase-mediated inhibition of oxidative phosphorylation

Development of an integrated multi-species and multi-dose route PBPK model for volatile methyl siloxanes – D4 and D5

AbstractThere are currently seven published physiologically based pharmacokinetic (PBPK) models describing aspects of the pharmacokinetics of octamethylcyclotetrasiloxane (D4) and decamethylcyclopentasiloxane (D5) for various exposure routes in rat and human. Each model addressed the biological and physico-chemical properties of D4 and D5 (highly lipophilic coupled with low blood: air partition coefficient and high liver clearance) that result in unique kinetic behaviors as well differences between D4 and D5. However, the proliferation of these models resulted in challenges for various risk assessment applications when needing to determine the optimum model for estimating dose metrics. To enhance the utility of these PBPK models for risk assessment, we integrated the suite of structures into one coherent model capable of simulating the entire set of existing data equally well as older more limited scope models. In this paper, we describe the steps required to develop this integrated model, the choice of physiological, partitioning and biochemical parameters for the model, and the concordance of the model behavior across key data sets. This integrated model is sufficiently robust to derive relevant dose metrics following individual or combined dermal and inhalation exposures of workers, consumer or the general population to D4 and D5 for route-to-route, interspecies and high to low dose extrapolations for risk assessment

The Effects of Physical Activity on Learning Behaviors in Elementary School Children: A Randomized Controlled Trial

This work is licensed under a Creative Commons Attribution 4.0 International License.Research in education and developmental psychology indicates that behavioral engagement in learning is a critical predictor of children’s academic success. In an effort to improve academic achievement, school administrators are continually in search of methods to increase behavioral engagement. Previous research has indicated that classroom-based physical activity (PA) lessons have a positive impact on academic achievement. However, little research has been done in assessing the impact of such interventions on the behavioral engagement of students with learning behavior difficulties. This study assesses the impact of classroom-based PA on teacher-rated classroom behaviors of students with identified learning behavior difficulties. Two schools (one intervention, one control) participating in a larger, cluster-randomized trial provided scores on a teacher-administered classroom behavior scale. This scale was used to collect information on 15 characteristics identified as being essential to behavioral engagement. Participants included male and female students in second and third grade classrooms who were identified by their classroom teacher and school counselor as having difficulties with learning behaviors. Mixed linear modeling for repeated measures was used to examine the changes over time in the classroom behavior scores. The intervention group showed significant improvement over time in classroom behavior while the control group showed no change or a slight degradation over time (i.e., group × time interaction, F[2132] = 4.52, p = 0.01). Schools must meet the diverse needs of students today, including those who exhibit less than optimal learning behaviors. Combined with the evidence that PA is linked to several health and cognitive-behavior benefits, providing classroom-based PA that is incorporated within the curriculum provides common ground for all students to participate. It is a potential solution to increasing behavioral engagement, and in turn stimulating and enhancing learning

Utah\u27s 1977 Drought

The Utah Drought of 1976-1977 has been characterized as the wettest drought in history. It was produced by the driest winter season of record followed by summer rains which were much wetter than normal in many regions of the state. Stream-flow is highly correlated with winter rather than summer precipitation and therefore the most severe impacts of the drought were related to the record low stream-flow during 1977 plus the ski industry impacts which were directly related to 1976-1977 snowfall. Drought impacts upon several sectors of the economy plus the extensive responses of all levels of government in the form of drought relief programs are described and quantified

Letter from J Harvey to Barry [J O Barry-Walsh]

Manuscript letter dated 10 March 1939, thanking Mr Barry-Walsh for membership forms and enclosing a list of members (not included)

Cartel Damages Claims, Passing-On and Passing-Back

Firms can mitigate the harm of an input cartel by passing on some of the overcharge to their customers through raising their own prices. Recent claims for damages have highlighted that firms may also respond by negotiating lower prices with their suppliers of other complementary inputs, thereby passing back some of the harm upstream. By analysing a model where downstream supply requires two inputs, we derive the equilibrium `passing-on' and `passing-back' effects when one input is cartelised. We show that the cartel causes a larger passing-back effect when there is greater market power in the complementary input sector. This reduces the passing-on effect. We find that the passing-back effect can inflict substantial harm on the complementary input suppliers and reduce the harm inflicted on direct and/or indirect purchasers