頭頸部癌PDXモデルを用いた新規バイオマーカーの確立と治療効果予測への応用

金沢大学附属病院【目的】頭頸部癌の患者腫瘍組織移植モデル（以下PDX）の有用性を検証する。【方法】実際の頭頸部癌患者とPDXモデルでのシスプラチンの薬剤感受性の相関や薬剤耐性にかかわるとされるABC-トランスポーター(MDR-1, MRP-2)の発現の腫瘍の継代や薬剤投与による変化について検討した。【結果】頭頸部癌PDXモデル5例を確立した。患者とPDXで組織型や免疫染色像は相似した。3例について患者とPDXでのシスプラチンの薬剤感受性を検討したところ相関がみられ、薬剤投与によるABC-トランスポーターの発現が増強するものもみられた。【結論】頭頸部癌PDXモデルは実際の癌患者の性質に相似する。【Objectives】We assessed the utility of PDXs as an oncology research platform for head and neck cancer (HNC).【Methods】We determined how the PDX responses to anti-cancer drugs mirrored the patients’clinical responses. Moreover, we investigated the expression of adenosine triphosphate-binding cassette (ABC)-transporter in accordance with chemotherapy in the HNC-PDX model. 【Results】 Five PDXs from HNC patients were established. These PDXs exhibited high accordance with the histopathological and immunohistochemical features of the original patient specimens. The responses of three PDXs to cisplatin correlated with the patients’ clinical response. Augmented ABC-transporter expression was observed in one PDX model after treatment with anti-cancer drugs, but not in passaged PDXs treated with phosphate-buffered saline. 【Conclusion】PDX models are feasible and exhibit similar biological and drug-response characteristics to primary tumors from patients.研究課題/領域番号:17K16899, 研究期間(年度):2017-04-01 – 2019-03-3

頭頸部癌PDXモデルを用いた新規バイオマーカーの確立と治療効果予測への応用

金沢大学附属病院【目的】頭頸部癌の患者腫瘍組織移植モデル（以下PDX）の有用性を検証する。【方法】実際の頭頸部癌患者とPDXモデルでのシスプラチンの薬剤感受性の相関や薬剤耐性にかかわるとされるABC-トランスポーター(MDR-1, MRP-2)の発現の腫瘍の継代や薬剤投与による変化について検討した。【結果】頭頸部癌PDXモデル5例を確立した。患者とPDXで組織型や免疫染色像は相似した。3例について患者とPDXでのシスプラチンの薬剤感受性を検討したところ相関がみられ、薬剤投与によるABC-トランスポーターの発現が増強するものもみられた。【結論】頭頸部癌PDXモデルは実際の癌患者の性質に相似する。【Objectives】We assessed the utility of PDXs as an oncology research platform for head and neck cancer (HNC).【Methods】We determined how the PDX responses to anti-cancer drugs mirrored the patients’clinical responses. Moreover, we investigated the expression of adenosine triphosphate-binding cassette (ABC)-transporter in accordance with chemotherapy in the HNC-PDX model. 【Results】 Five PDXs from HNC patients were established. These PDXs exhibited high accordance with the histopathological and immunohistochemical features of the original patient specimens. The responses of three PDXs to cisplatin correlated with the patients’ clinical response. Augmented ABC-transporter expression was observed in one PDX model after treatment with anti-cancer drugs, but not in passaged PDXs treated with phosphate-buffered saline. 【Conclusion】PDX models are feasible and exhibit similar biological and drug-response characteristics to primary tumors from patients.研究課題/領域番号:17K16899, 研究期間(年度):2017-04-01 – 2019-03-31出典：研究課題「頭頸部癌PDXモデルを用いた新規バイオマーカーの確立と治療効果予測への応用」課題番号17K16899（KAKEN：科学研究費助成事業データベース（国立情報学研究所）） （https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-17K16899/17K16899seika/）を加工して作

Amino Acid Composition of Amniotic Fluid during the Perinatal Period Reflects Mother’s Fat and Carbohydrate Intake

Dietary content during pregnancy is important because it is necessary for the growth of the fetus. With the assumption that the nutritional status of the fetus can be monitored by measuring amino acid concentrations in the amniotic fluid, we investigated whether the habitual dietary intake of pregnant women affected the composition of the amniotic fluid and the significance of performing amniotic fluid analysis. The subjects were 34 mothers who delivered full-term babies by cesarean section. Three biological samples were collected from the mothers: blood, cord blood, and amniotic fluid. At the same time, the mothers’ prenatal nutritional intake information was also recorded. When the amino acid contents of the samples were compared with the mothers’ nutrient intake, many amino acids in the amniotic fluid were positively correlated with lipid intake, but not with protein intake. There was a negative correlation between lipid intake and carbohydrate intake, and the amino acid contents of the amniotic fluid were also negatively correlated with carbohydrate intake. The results of this study were consistent with those found in animal models, suggesting that the analysis of amniotic fluid may be a useful method to investigate the effects of habitual diet during human pregnancy on the fetus