Conjugative transfer frequencies of mef(A)-containing Tn1207.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types

The aim of this study was to examine the gene transfer potential of mef(A)-containing Tn120.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types. Using the filter mating technique, Tn1207.3 was transferred by conjugation to 23 macrolide-susceptible recipients representing 11 emm types. PCR analysis confirmed the presence of the mef(A) gene and the comEC junction regions of the Tn1207.3 insertion in resultant transconjugants. Significant variation was found in the transfer frequency of Tn1207.3 to different Strep. pyogenes strains, and this phenomenon may contribute to the differences in mef(A) frequency observed among clinical isolates. Significance and Impact of the Study: The spread of antimicrobial resistance among pathogenic bacteria is an important problem, but the mechanisms of horizontal transfer between strains and species are often poorly understood. For instance, little is known on how macrolide resistance spreads between strains of the human pathogen Strep. pyogenes and why certain strains more commonly display resistance than others. Here, we show that Strep. pyogenes strains vary greatly in their ability to acquire a transposon encoding macrolide resistance by horizontal gene transfer in vitro. These data provide a novel insight into the transfer of antibiotic resistance between bacterial strains and offer an explanation for the differences in the frequency of resistance determinates and resistance seen among clinical isolates. © 2014 The Authors Letters in Applied Microbiology

17O NMR study of the intrinsic magnetic susceptibility and spin dynamics of the quantum kagome antiferromagnet ZnCu3(OH)6Cl2

We report through 17O NMR, an unambiguous local determination of the intrinsic kagome lattice spin susceptibility as well as that created around non-magnetic defects issued from natural Zn/ Cu exchange in the S=1/2 (Cu2+) herbertsmithite ZnCu3(OH)6Cl2 compound. The issue of a singlet-triplet gap is addressed. The magnetic response around a defect is found to markedly differ from that observed in non-frustrated antiferromagnetic materials. Finally, we discuss our relaxation measurements in the light of Cu and Cl NMR data [cond-mat 070314] and suggest a flat q-dependence of the excitations.Comment: Accepted for publication in Phys. Rev. Lett., 3 jan. 2008 Figure 1 has been modified to include a two-components fit of the 17O NMR spectru

Vorticity production and survival in viscous and magnetized cosmologies

We study the role of viscosity and the effects of a magnetic field on a rotating, self-gravitating fluid, using Newtonian theory and adopting the ideal magnetohydrodynamic approximation. Our results confirm that viscosity can generate vorticity in inhomogeneous environments, while the magnetic tension can produce vorticity even in the absence of fluid pressure and density gradients. Linearizing our equations around an Einstein-de Sitter cosmology, we find that viscosity adds to the diluting effect of the universal expansion. Typically, however, the dissipative viscous effects are confined to relatively small scales. We also identify the characteristic length bellow which the viscous dissipation is strong and beyond which viscosity is essentially negligible. In contrast, magnetism seems to favor cosmic rotation. The magnetic presence is found to slow down the standard decay-rate of linear vortices, thus leading to universes with more residual rotation than generally anticipated.Comment: Minor changes. References added and updated. Published versio

Spatial Relationship between Solar Flares and Coronal Mass Ejections

We report on the spatial relationship between solar flares and coronal mass ejections (CMEs) observed during 1996-2005 inclusive. We identified 496 flare-CME pairs considering limb flares (distance from central meridian > 45 deg) with soft X-ray flare size > C3 level. The CMEs were detected by the Large Angle and Spectrometric Coronagraph (LASCO) on board the Solar and Heliospheric Observatory (SOHO). We investigated the flare positions with respect to the CME span for the events with X-class, M-class, and C-class flares separately. It is found that the most frequent flare site is at the center of the CME span for all the three classes, but that frequency is different for the different classes. Many X-class flares often lie at the center of the associated CME, while C-class flares widely spread to the outside of the CME span. The former is different from previous studies, which concluded that no preferred flare site exists. We compared our result with the previous studies and conclude that the long-term LASCO observation enabled us to obtain the detailed spatial relation between flares and CMEs. Our finding calls for a closer flare-CME relationship and supports eruption models typified by the CSHKP magnetic reconnection model.Comment: 7 pages; 4 figures; Accepted by the Astrophysical Journa

Disentangling the Complex Broadband X-ray Spectrum of IRAS 13197-1627 with NuSTAR, XMM-Newton and Suzaku

We present results from a coordinated $XMM$-$Newton$+$NuSTAR$ observation of the type 1.8 Seyfert galaxy IRAS 13197-1627. This is a highly complex source, with strong contributions from relativistic reflection from the inner accretion disk, neutral absorption and further reprocessing by more distant material, and ionised absorption from an outflow. We undertake a detailed spectral analysis combining the broadband coverage provided by $XMM$-$Newton$+$NuSTAR$ with a multi-epoch approach incorporating archival observations performed by $XMM$-$Newton$ and $Suzaku$. Our focus is on characterising the reflection from the inner accretion disk, which previous works have suggested may dominate the AGN emission, and constraining the black hole spin. Using lamppost disk reflection models, we find that the results for the inner disk are largely insensitive to assumptions regarding the geometry of the distant reprocessor and the precise form of the illuminating X-ray continuum. However, these results do depend on the treatment of the iron abundance of the distant absorber/reprocessor. The multi-epoch data favour a scenario in which the AGN is chemically homogeneous, and we find that a rapidly rotating black hole is preferred, with $a^* \geq 0.7$, but a slowly-rotating black hole is not strongly excluded. In addition to the results for the inner disk, we also find that both the neutral and ionised absorbers vary from epoch to epoch, implying that both have some degree of inhomogeneity in their structure.Comment: 15 pages, 7 figures, accepted for publication in MNRA

The Stability of Polar Oxide Surfaces

The structures of the polar surfaces of ZnO are studied using ab initio calculations and surface x-ray diffraction. The experimental and theoretical relaxations are in good agreement. The polar surfaces are shown to be very stable; the cleavage energy for the (0001)-Zn and (0001̅ )-O surfaces is 4.0J/m2 comparable to 2.32J/m2 for the most stable nonpolar (1010) surface. The surfaces are stabilized by an electronic mechanism involving the transfer of 0.17 electrons between them. This leads to 2D metallic surface states, which has implications for the use of the material in gas sensing and catalytic applications

NGC 1068: No change in the mid-IR torus structure despite X-ray variability

Context. Recent NuSTAR observations revealed a somewhat unexpected increase in the X-ray flux of the nucleus of NGC 1068. We expect the infrared emission of the dusty torus to react on the intrinsic changes of the accretion disk. Aims. We aim to investigate the origin of the X-ray variation by investigating the response of the mid-infrared environment. Methods. We obtained single-aperture and interferometric mid-infrared measurements and directly compared the measurements observed before and immediately after the X-ray variations. The average correlated and single-aperture fluxes as well as the differential phases were directly compared to detect a possible change in the structure of the nuclear emission on scales of $\sim$ 2 pc. Results. The flux densities and differential phases of the observations before and during the X-ray variation show no significant change over a period of ten years. Possible minor variations in the infrared emission are $\lesssim$ 8 %. Conclusions. Our results suggest that the mid-infrared environment of NGC 1068 has remained unchanged for a decade. The recent transient change in the X-rays did not cause a significant variation in the infrared emission. This independent study supports previous conclusions that stated that the X-ray variation detected by NuSTAR observations is due to X-ray emission piercing through a patchy section of the dusty region.Comment: 6 pages, 5 figures, 3 tables. Accepted for publication on A&

Vacuum Alignment in SUSY A4 Models

In this note we discuss the vacuum alignment in supersymmetric models with spontaneously broken flavour symmetries in the presence of soft supersymmetry (SUSY) breaking terms. We show that the inclusion of soft SUSY breaking terms can give rise to non-vanishing vacuum expectation values (VEVs) for the auxiliary components of the flavon fields. These non-zero VEVs can have an important impact on the phenomenology of this class of models, since they can induce an additional flavour violating contribution to the sfermion soft mass matrix of right-left (RL) type. We carry out an explicit computation in a class of SUSY A4 models predicting tri-bimaximal mixing in the lepton sector. The flavour symmetry breaking sector is described in terms of flavon and driving supermultiplets. We find non-vanishing VEVs for the auxiliary components of the flavon fields and for the scalar components of the driving fields which are of order m_{SUSY} x and m_{SUSY}, respectively. Thereby, m_{SUSY} is the generic soft SUSY breaking scale which is expected to be around 1 TeV and is the VEV of scalar components of the flavon fields. Another effect of these VEVs can be the generation of a mu term.Comment: 23 pages; added a new section on the relation to Supergravity; version accepted for publication in JHE

Fractional Chern insulator on a triangular lattice of strongly correlated t2gt_{2g} electrons

We discuss the low-energy limit of three-orbital Kondo-lattice and Hubbard models describing $t_{2g}$ orbitals on a triangular lattice near half-filling. We analyze how very flat bands with non-trivial topological character, a Chern number C=1, arise both in the limit of infinite on-site interactions as well as in more realistic regimes. Exact diagonalization is then used to investigate fractional filling of an effective one-band spinless-fermion model including nearest-neighbor interaction $V$; it reveals signatures of fractional Chern insulators (FCIs) for several filling fractions. In addition to indications based on energies, e.g. flux insertion and fractional statistics of quasiholes, Chern numbers are obtained. It is shown that FCIs are robust against disorder in the underlying magnetic texture that defines the topological character of the band. We also investigate competition between FCI states and a charge density wave (CDW) and discuss particle-hole asymmetry as well as Fermi-surface nesting. FCI states turn out to be rather robust and do not require very flat bands, but can also arise when filling or an absence of Fermi-surface nesting disfavor the competing CDW. Nevertheless, very flat bands allow FCI states to be induced by weaker interactions than those needed for more dispersive bands.Comment: 14 pages, 13 figure