8 research outputs found
Structural adaptations in the murine colon microcirculation associated with haptenâinduced inflammation
BACKGROUND: Blood flowing across the vascular endothelium creates wall shear stress, dependent on velocity of flow and vessel geometry, that tends to disrupt lymphocyteâendothelial cell adhesion. OBJECTIVE: The microcirculation in a murine model of acute colitis was investigated to identify structural adaptations during acute colitis that may facilitate transmigration. METHODS: In 2,4,6âtrinitrobenzenesulphonic acidâinduced acute colitis, the infiltrating cells and colonic microcirculation was investigated by cellular topographic mapping, corrosion casting and threeâdimensional scanning electron microscopy (SEM). Colonic blood velocimetry was performed using intravital microscopy. RESULTS: Clinical and histological parameters suggested a peak inflammatory response at 96â
h (p<0.001). The infiltrating cells were spatially related to the mucosal capillary plexus by threeâdimensional topographic mapping (p<0.001). In normal mice, corrosion casting and threeâdimensional SEM showed a polygonal mucosal plexus supplied by ascending arteries and descending veins. After 2,4,6âtrinitrobenzenesulphonic acid stimulation, threeâdimensional SEM showed preserved branch angles (pâ=â0.52) and nominal vessel lengths (pâ=â0.93), but a significantly dilated mucosal capillary plexus (p<0.001). Intravital microscopy of the mucosal plexus showed a greater than twofold decrease in the velocity of flow (p<0.001). CONCLUSIONS: The demonstrable slowing of the velocity of flow despite an increase in volumetric flow suggests that these microvascular adaptations create conditions suitable for leucocyte adhesion and transmigration