Detection of DNA and Poly-L-Lysine using CVD Graphene-channel FET Biosensors

A graphene channel field-effect biosensor is demonstrated for detecting the binding of double-stranded DNA and poly-l-lysine. Sensors consist of CVD graphene transferred using a clean, etchant-free transfer method. The presence of DNA and poly-l-lysine are detected by the conductance change of the graphene transistor. A readily measured shift in the Dirac Voltage (the voltage at which the graphenes resistance peaks) is observed after the graphene channel is exposed to solutions containing DNA or poly-l-lysine. The Dirac voltage shift is attributed to the binding/unbinding of charged molecules on the graphene surface. The polarity of the response changes to positive direction with poly-l-lysine and negative direction with DNA. This response results in detection limits of 8 pM for 48.5 kbp DNA and 11 pM for poly-l-lysine. The biosensors are easy to fabricate, reusable and are promising as sensors of a wide variety of charged biomolecule

Low-power photothermal self-oscillation of bimetallic nanowires

We investigate the nonlinear mechanics of a bimetallic, optically absorbing SiN-Nb nanowire in the presence of incident laser light and a reflecting Si mirror. Situated in a standing wave of optical intensity and subject to photothermal forces, the nanowire undergoes self-induced oscillations at low incident light thresholds of $<1\, \rm{\mu W}$ due to engineered strong temperature-position ($T$-$z$) coupling. Along with inducing self-oscillation, laser light causes large changes to the mechanical resonant frequency $\omega_0$ and equilibrium position $z_0$ that cannot be neglected. We present experimental results and a theoretical model for the motion under laser illumination. In the model, we solve the governing nonlinear differential equations by perturbative means to show that self-oscillation amplitude is set by the competing effects of direct $T$-$z$ coupling and $2\omega_0$ parametric excitation due to $T$-$\omega_0$ coupling. We then study the linearized equations of motion to show that the optimal thermal time constant $\tau$ for photothermal feedback is $\tau \to \infty$ rather than the widely reported $\omega_0 \tau = 1$. Lastly, we demonstrate photothermal quality factor ($Q$) enhancement of driven motion as a means to counteract air damping. Understanding photothermal effects on micromechanical devices, as well as nonlinear aspects of optics-based motion detection, can enable new device applications as oscillators or other electronic elements with smaller device footprints and less stringent ambient vacuum requirements.Comment: New references adde

Evanescent field optical readout of graphene mechanical motion at room temperature

Graphene mechanical resonators have recently attracted considerable attention for use in precision force and mass sensing applications. To date, readout of their oscillatory motion has typically required cryogenic conditions to achieve high sensitivity, restricting their range of applications. Here we report the first demonstration of evanescent optical readout of graphene motion, using a scheme which does not require cryogenic conditions and exhibits enhanced sensitivity and bandwidth at room temperature. We utilise a high $Q$ microsphere to enable evanescent readout of a 70 $\mu$m diameter graphene drum resonator with a signal-to-noise ratio of greater than 25 dB, corresponding to a transduction sensitivity of $S_{N}^{1/2} =$ 2.6 $\times 10^{-13}$ m $\mathrm{Hz}^{-1/2}$. The sensitivity of force measurements using this resonator is limited by the thermal noise driving the resonator, corresponding to a force sensitivity of $F_{min} = 1.5 \times 10^{-16}$ N ${\mathrm{Hz}}^{-1/2}$ with a bandwidth of 35 kHz at room temperature (T = 300 K). Measurements on a 30 $\mu$m graphene drum had sufficient sensitivity to resolve the lowest three thermally driven mechanical resonances.Comment: Fixed formatting errors in bibliograph

Synchronous imaging for rapid visualization of complex vibration profiles in electromechanical microresonators

Synchronous imaging is used in dynamic space-domain vibration profile studies of capacitively driven, thin n+ doped poly-silicon microbridges oscillating at rf frequencies. Fast and high-resolution actuation profile measurements of micromachined resonators are useful when significant device nonlinearities are present. For example, bridges under compressive stress near the critical Euler value often reveal complex dynamics stemming from a state close to the onset of buckling. This leads to enhanced sensitivity of the vibration modes to external conditions, such as pressure, temperatures, and chemical composition, the global behavior of which is conveniently evaluated using synchronous imaging combined with spectral measurements. We performed an experimental study of the effects of high drive amplitude and ambient pressure on the resonant vibration profiles in electrically-driven microbridges near critical buckling. Numerical analysis of electrostatically driven post-buckled microbridges supports the richness of complex vibration dynamics that are possible in such micro-electromechanical devices.Comment: 7 pages, 8 figure, submitted to Physical Review

Defining NELF-E RNA binding in HIV-1 and promoter-proximal pause regions

The four-subunit Negative Elongation Factor (NELF) is a major regulator of RNA Polymerase II (Pol II) pausing. The subunit NELF-E contains a conserved RNA Recognition Motif (RRM) and is proposed to facilitate Poll II pausing through its association with nascent transcribed RNA. However, conflicting ideas have emerged for the function of its RNA binding activity. Here, we use in vitro selection strategies and quantitative biochemistry to identify and characterize the consensus NELF-E binding element (NBE) that is required for sequence specific RNA recognition (NBE: CUGAGGA(U) for Drosophila). An NBE-like element is present within the loop region of the transactivation-response element (TAR) of HIV-1 RNA, a known regulatory target of human NELF-E. The NBE is required for high affinity binding, as opposed to the lower stem of TAR, as previously claimed. We also identify a non-conserved region within the RRM that contributes to the RNA recognition of Drosophila NELF-E. To understand the broader functional relevance of NBEs, we analyzed promoter-proximal regions genome-wide in Drosophila and show that the NBE is enriched +20 to +30 nucleotides downstream of the transcription start site. Consistent with the role of NELF in pausing, we observe a significant increase in NBEs among paused genes compared to non-paused genes. In addition to these observations, SELEX with nuclear run-on RNA enrich for NBE-like sequences. Together, these results describe the RNA binding behavior of NELF-E and supports a biological role for NELF-E in promoter-proximal pausing of both HIV-1 and cellular genes

Graphene Metallization of High-Stress Silicon Nitride Resonators for Electrical Integration

High stress stoichiometric silicon nitride resonators, whose quality factors exceed one million, have shown promise for applications in sensing and signal processing. Yet, electrical integration of the insulating silicon nitride resonators has been challenging, as depositing even a thin layer of metal degrades the quality factor significantly. In this work, we show that graphene used as a conductive coating for Si3N4 membranes reduces the quality factor by less than 30 % on average, which is minimal when compared to the effect of conventional metallization layers such as chromium or aluminum. The electrical integration of Si3N4-Graphene (SiNG) heterostructure resonators is demonstrated with electrical readout and electro-static tuning of the frequency by up to 1 % per volt. These studies demonstrate the feasibility of hybrid graphene/nitride mechanical resonators in which the electrical properties of graphene are combined with the superior mechanical performance of silicon nitride.Comment: 8 pages, 5 figures, journa

An Array of Planar Apertures for Near-Field Fluorescence Correlation Spectroscopy

We have developed a method of performing near-field fluorescence correlation spectroscopy via an array of planarized circular apertures of 50 nm diameter. This technique provides 1 μs and 60 nm resolution on proximal samples, including live cells, without incorporating a scanning probe or pulsed lasers or requiring penetration of the sample into the aperture. Millions of apertures are created in an array within a thin film of aluminum on a coverslip and planarized to achieve no height distinction between the apertures and the surrounding metal. Supported lipid bilayers and plasma membranes from live cells adhere to the top of this substrate. We performed fluorescence correlation spectroscopy to demonstrate the sub-diffraction-limited illumination with these devices