Variables Influencing Differences in Sequence Conservation in the Fission Yeast Schizosaccharomyces pombe

Which variables determine the constraints on gene sequence evolution is one of the most central questions in molecular evolution. In the fission yeast Schizosaccharomyces pombe, an important model organism, the variables influencing the rate of sequence evolution have yet to be determined. Previous studies in other single celled organisms have generally found gene expression levels to be most significant, with numerous other variables such as gene length and functional importance identified as having a smaller impact. Using publicly available data, we used partial least squares regression, principal components regression, and partial correlations to determine the variables most strongly associated with sequence evolution constraints. We identify centrality in the protein-protein interactions network, amino acid composition, and cellular location as the most important determinants of sequence conservation. However, each factor only explains a small amount of variance, and there are numerous variables having a significant or heterogeneous influence. Our models explain more than half of the variance in dN, raising the possibility that future refined models could quantify the role of stochastics in evolutionary rate variation

Individual demand for education

La p. de t. du v.2 porte en outre: France, Germany, Greece, United KingdomVol.1 Rapport analytique / Kjell Hèarnqvist -- v.2 Rapport général et études de cas / Organisation de coopération et de développement économique

desync-cc: A research tool for automatically applying disassembly desynchronization during compilation

Code obfuscation is an important topic, both in terms of defense, when trying to prevent intellectual property theft, and from the offensive point of view, when trying to break obfuscation used in malware. Several recent works have discussed techniques for preventing or delaying reverse engineering of binaries. While most works focus on methods that obscure program logic, the complimentary approach of disassembly desynchronizationhas received relatively little attention, despite being often used by, for example, malware authors. The technique puts another hurdle in the way of attackers by targeting the most fundamental step of the reverse-engineering process: recovering assembly code from a program binary. In the interest of furthering research into this kind of obfuscation, we present desync-cc, a tool for automatic application of disassembly desynchronization. To facilitate maximal ease-of-use, the tool is designed as a drop-in replacement for gcc, and works by intercepting and modifying intermediate assembly-code during compilation. (c) 2023 The Authors. Published by Elsevier B.V