Reading the Room: Memory, Dwelling, and the Everyday

In any space, there is a residue that coats the present with a patina of memory. Creating layered imagery in dream-like paintings and prints, I use the domestic realm as a metaphor for the internal world of the mind, memories, and private thoughts, including them in compositions with symbols like the boundaries of windows, doors, and gates. These metaphorical structures also portray outward identities, which guard inner emotions. The conceptual aspects of these compositional elements weave together memories of the past and places of the present into a unified whole. I began graduate school at the beginning of the COVID-19 pandemic. I often found myself observing different dwellings within and outside of my personal spaces. My intention is to show the connection between our dwellings and minds and how the environment can influence a mental state

Genome-Wide Analysis of Light- and Temperature-Entrained Circadian Transcripts in Caenorhabditis elegans

Transcriptional profiling experiments identify light- and temperature-entrained circadian transcripts in C. elegans

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

When water is from God: formation of property rights governing communal irrigation furrows in Meru, Tanzania, c. 1890-2011

In Meru, Tanzania local initiatives were instrumental in establishing a gravity irrigation system in the 1890s. The original property rights institutions governing furrows were characterised by de facto communal ownership and management combined with private temporary user rights. Over the last 12 decades farming systems in Meru have experienced changing land/labour ratios, overall technological and institutional change as well as increased demand for irrigation water. The furrow system has been extended and due to general agricultural intensification access to water has become an important pre-condition for production in the current local system of agricultural production. However, it is argued that in the midst of drastic overall change in the area, irrigation furrows have experienced no significant change in either technology or property rights institutions. It is found that institutional continuity is explained by the natural characteristics of water, property rights embeddedness in socio-economic structures, and challenges of managing it as a common-pool resource