Brachypodium distachyon as a model for defining the allergen potential of non-prolamin proteins

Epitope databases and the protein sequences of published plant genomes are suitable to identify some of the proteins causing food allergies and sensitivities. Brachypodium distachyon, a diploid wild grass with a sequenced genome and low prolamin content, is the closest relative of the allergen cereals, such as wheat or barley. Using the Brachypodium genome sequence, a workflow has been developed to identify potentially harmful proteins which may cause either celiac disease or wheat allergy-related symptoms. Seed tissue-specific expression of the potential allergens has been determined, and intact epitopes following an in silico digestion with several endopeptidases have been identified. Molecular function of allergen proteins has been evaluated using Gene Ontology terms. Biologically overrepresented proteins and potentially allergen protein families have been identified. © 2012 The Author(s)

Application of Micro Z-arm dough mixer in wheat research - Effect of protein addition on mixing properties of wheat dough

A Micro Z-arm mixer has been developed which needs only four grams of flour per test. The new instrument is suitable for screening new wheat cultivars in the early phase of selection on the basis of dough mixing properties. The micro-scale instrument can also be viewed as a valuable research tool for investigation of the structure/function relationships in flour and the effects of different ingredients and additives on rheological parameters. The effects of protein addition on mixing properties of wheat dough were studied. Alkali-soluble isolates and Osborne protein fractions extracted from wheat, corn-, rice germs and amaranth seeds were used as protein additives. Dough development time, maximum resistance of dough and resistance breakdown was calculated from recorded mixing curves. Results showed that the micro-scale equipment is sensitive enough for detection of the effect of less than 40 mg of protein addition on mixing parameters. The effects of additions depend on the type of proteins added to the wheat flour and the parameters calculated from the mixing curves

Brachypodium distachyon as a model for defining the allergen potential of non-prolamin proteins

Epitope databases and the protein sequences of published plant genomes are suitable to identify some of the proteins causing food allergies and sensitivities. Brachypodium distachyon, a diploid wild grass with a sequenced genome and low prolamin content, is the closest relative of the allergen cereals, such as wheat or barley. Using the Brachypodium genome sequence, a workflow has been developed to identify potentially harmful proteins which may cause either celiac disease or wheat allergy-related symptoms. Seed tissue-specific expression of the potential allergens has been determined, and intact epitopes following an in silico digestion with several endopeptidases have been identified. Molecular function of allergen proteins has been evaluated using Gene Ontology terms. Biologically overrepresented proteins and potentially allergen protein families have been identified. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10142-012-0294-z) contains supplementary material, which is available to authorized users

Proteomic analysis relevant to cereal grains

Proteomics complements the analysis of gene expression by analyzing all the polypeptides (proteins) expressed by an organism's genome in a specific plant or grain tissue at a particular stage of development and under defined growth conditions. Diverse technologies have been developed for the fractionation and identification of the individual polypeptides that constitute the proteome. Information resulting from proteomics is being used to facilitate novel and conventional plant breeding

Spelt (Triticum spelta L.) in Vitro Androgenesis Breeding for Special Food Quality Parameters

János Pauk, Csaba Lantos, Katalin Ács, Gyöngyvér Gell, Sándor Tömösközi, Kornélia Hajdú Búza, Ferenc Béké