Analisis Asam Valproat Dalam Plasma Secara Kromatografi Gas

Valproic acid is an anticonvulsant drug that works by increasing the levels ofγ-aminobutyric acid (GABA). Determination of valproic acid is quite difficultbecause it has no chromophore groups in its structure. Aa analytical method usinggas chromatography (GC) with flame ionization detector for the determination ofvalproic acid in human plasma has been developed and optimized. Valproic acid wasextracted from plasma by liquid-liquid extraction method using diethyl ether. Theoptimum analysis conditions for valproic acid in plasma were achieved by regulatedgas chromatography injector and detector at a temperature of 250oC and temperatureprogramming with an initial temperature of 70oC and 5oC temperature increasingper minute until a temperature of 100oC, then held for 1 minute. Then the temperaturewas increased by 2°C per minute until the column temperature to 150oC. Theoptimum conditions of analysis took 32 minutes. In the concentration range from40.0 to 100.0 µg/mL yielded a linear calibration curve with correlation coefficient (r)of 0.9894. Accuracy (% diff) of this method was -13.67% to 12.33% with precision(CV) between 9.33% to 14.92%, and relative recovery test was 86.33% to 112.33%

Analisis Glimepirida Dalam Plasma Tikus

The aim of this research is to find the method for analyze glimepiride and itÂ’s metabolite. Glimepiride is the second generation of antidiabetic oral from the sulphonyl urea that works by stimulating the insulin secretion from beta cells of pancreas. Glimepiride is isolated from plasma the using chloroform. Using the high performance liquid chromatography method which include C18 reversed phase column, using mixture of methanol:water (50:50, v/v) as a mobile phase, flow rate 1.0 ml/minutes, detection at wavelenght 228 nm with photo diode array detector gives retention times of glimepiride in 17 minutes without any interference from endogen component of plasma and from itÂ’s metabolite. Linearity with added internal standard gliclazide was established for the range concentration 100-1000 ng/ml with coefficient of correlation (r) is 0.9977 and give the limit of quantitation of glimepiride in 50 ng/ml. The results of validation method fulfilled for the given criterias

Validasi Metode Analisis Cilostazol Dalam Plasma in Vitro Secara Kromatografi Cair Kinerja Tinggi

Cilostazol is an antiplatelet agent with the mechanism of action by inhibiting phos-phodiesterase III (PDE III). Referred to Food and Drug Administration(FDA),cilostazol is a drug recommended to be bioequivalence (BE) studied. A high-perfor-mance liquid chromatographic (HPLC) method with ultraviolet detector for in vitro determination of cilostazol in human plasma had been developed and validated.Cilostazol and pioglitazone as internal standard were extracted from human plasma by protein precipitation method using methanol. The mobile phase consisting of ac-etonitrile-potassium di-hydrogen phosphate buffer 50 mM (40:60) was used at the flow rate of 1.5 mL/min on reversed phase C18 column (SunfireTM, 5 µm, 250x4.6 mm), and was detected at wavelength of 257 nm. Linearity was established withinconcentration range of 20-2000 ng/mL with coefficient correlation (r) was 0,9999.Accuracy (% diff) of this method was -14.67% up to 8.84% with precision (CV) being 0.98% to 4.93%, and absolute recovery was established to be 82.26% to 119.85%.Cilostazol in plasma was stable for 30 days in -200C storage

Analisis Adduct DNA Setelah Pemberian Natrium Nitrit Dan Dimetilamin Secara Berulang Pada Tikus

Nitrosodimethylamine is a carcinogenic compound which can be formed from the reaction of nitrite and dimethylamine that is found in food. Nitrosodimethylamineis activated in liver and alkylates the DNA base and producing a DNA adductssuch as O6-methylguanine and N7-methylguanine that have a role incarcinogenesis. In this research, DNA was isolated from rat’s blood which waspreviously given nitrosodimethylamine’s precursor, sodium nitrite anddimethylamine. DNA adducts can be obtained from hydrolysis in hydrochloricacid 0.1 N for 30 minutes at 7000C. Then the adducts were analyzed using High Performance Liquid Chromatography (HPLC), with a strong cation exchangecolumn (Supelcosil LC-SCX, 5 μm, 250 x 4.6 mm), mobile phase consisting ofammonium phosphate with a final concentration of 40 mM, pH 3.00, flow rate 1.5mL/minute, column temperature 30oC and detected at exitation wavelength 286 nm and emission wavelength 366 nm. This method gave an acceptable validation result according to accuracy and precicion test results that fulfill the requirementand linear calibration curve with a quantitation limit of 22,5403 ng/mL. Rats were divided into six groups that two groups were given nitrosodimethylamine aspositive control, three groups were given prekursor, and the other was normalcontrol.Blood samples were collected in 1,2 and 4 hour after last induced. Aftergiving sodium nitrite 110 mg/kg bw and dimethylamine (1:5) orally for a week,N7-methylguanine and O6-methylguanine had not been detected in rat’s blood

Implementation of a chat application for developers

Ofloksasin merupakan salah satu antibiotika golongan fluorokuinolon generasi kedua. Konsentrasi ofloksasin dalam plasma kecil, sehingga diperlukan metode analisis yang selektif, akurat, dan sensitif. Pada penelitian ini, dilakukan optimasi dan validasi metode analisis ofloksasin dalam plasma in vitro menggunakan kromatografi cair kinerja tinggi deteksi fluoresensi dengan siprofloksasin HCl sebagai baku dalam. Pemisahan dilakukan dengan menggunakan kolom C18 (Waters, SunfireTM 5 µm; 250 x 4,6 mm) dengan fase gerak isokratik yang terdiri dari trietilamin 1% dalam air pH 3,0–asetonitril (84:16), laju alir 1,0 mL/menit, suhu kolom 40oC, dan deteksi ofloksasin dilakukan pada panjang gelombang eksitasi 300 nm dan emisi 500 nm. Proses ekstraksi plasma dilakukan dengan metode pengendapan protein menggunakan metanol melalui proses vorteks selama 2 menit dan sentrifugasi pada kecepatan 10000 rpm selama 10 menit. Validitas dan linearitas metode analisis berada pada rentang konsentrasi 21,4 ng/mL – 4280 ng/mL dengan LLOQ 21,4 ng/mL. Nilai % diff dan koefisien variasi untuk akurasi dan presisi intra hari dan antar hari tidak lebih dari + 20% untuk konsentrasi LLOQ dan + 15% untuk konsentrasi rendah, sedang, dan tinggi. Ofloksasin dalam plasma dinyatakan stabil selama 3 siklus beku dan cair, stabil minimal 24 jam pada suhu kamar dan selama minimal 28 hari pada suhu -20oC. Metode analisis ofloksasin dalam plasma ini sudah memenuhi kriteria penerimaan sesuai persyaratan validasi pedoman EME

Edukasi dan Sosialisasi Vaksin Covid-19 Puskesmas Citeureup, Jawa Barat

Angka penularan penyakit COVID-19 di dunia masih tinggi dan menjadi perhatian dunia termasuk Indonesia dengan angka penularan yang relatif masih terbilang tinggi hingga pada saat ini. Pemerintah menggelontorkan biaya yang besar untuk melaksanakan program vaksinasi kepada seluruh masyarakat Indonesia secara gratis yang bertujuan untuk menurunkan angka penularan penyakit COVID-19 di Indonesia. Tujuan pengabdian untuk mengedukasi masyarakat tentang vaksinasi COVID-19, meyakinkan masyarakat tentang pentingnya melakukan vaksinasi dan menyukseskan program vaksinasi yang dilakukan oleh Pemerintah. Metode pelaksanaan yang telah dilakukan adalah sosialisasi melalui presentasi dan penyebaran flayer informasi vaksin, serta penyuluhan informasi terkait vaksin dan aspek terapeutik vaksinasi kepada 300 peserta yang bekerja sama dengan tim vaksinator Puskesmas Citeureup di puskesmas Citeureup, Jawa Barat. Maka dapat disimpulkan bahwa dengan adanya sosialisasi dan edukasi vaksinasi ini, warga mendapatkan manfaat, kepercayaan diri, pengetahuan terhadap jenis vaksin yang diberikan, proses mengatasi efek kejadian setelah vaksin serta peningkatan kesadaran terhadap protokol kesehatan. Sehingga kegiatan ini dapat membantu pemerintah untuk menekan tingkat penularan COVID-19 di wilayah Kecamatan Citeureup, Jawa Barat