Habitat Elevation Shapes Microbial Community Composition and Alter the Metabolic Functions in Wild Sable (Martes zibellina) Guts

In recent decades, wild sable (Carnivora Mustelidae Martes zibellina) habitats, which are often natural forests, have been squeezed by anthropogenic disturbances such as clear-cutting, tilling and grazing. Sables tend to live in sloped areas with relatively harsh conditions. Here, we determine effects of environmental factors on wild sable gut microbial communities between high and low altitude habitats using Illumina Miseq sequencing of bacterial 16S rRNA genes. Our results showed that despite wild sable gut microbial community diversity being resilient to many environmental factors, community composition was sensitive to altitude. Wild sable gut microbial communities were dominated by Firmicutes (relative abundance 38.23%), followed by Actinobacteria (30.29%), and Proteobacteria (28.15%). Altitude was negatively correlated with the abundance of Firmicutes, suggesting sable likely consume more vegetarian food in lower habitats where plant diversity, temperature and vegetation coverage were greater. In addition, our functional genes prediction and qPCR results demonstrated that energy/fat processing microorganisms and functional genes are enriched with increasing altitude, which likely enhanced metabolic functions and supported wild sables to survive in elevated habitats. Overall, our results improve the knowledge of the ecological impact of habitat change, providing insights into wild animal protection at the mountain area with hash climate conditions

Habitat Elevation Shapes Microbial Community Composition and Alter the Metabolic Functions in Wild Sable (Martes zibellina) Guts

In recent decades, wild sable (Carnivora Mustelidae Martes zibellina) habitats, which are often natural forests, have been squeezed by anthropogenic disturbances such as clear-cutting, tilling and grazing. Sables tend to live in sloped areas with relatively harsh conditions. Here, we determine effects of environmental factors on wild sable gut microbial communities between high and low altitude habitats using Illumina Miseq sequencing of bacterial 16S rRNA genes. Our results showed that despite wild sable gut microbial community diversity being resilient to many environmental factors, community composition was sensitive to altitude. Wild sable gut microbial communities were dominated by Firmicutes (relative abundance 38.23%), followed by Actinobacteria (30.29%), and Proteobacteria (28.15%). Altitude was negatively correlated with the abundance of Firmicutes, suggesting sable likely consume more vegetarian food in lower habitats where plant diversity, temperature and vegetation coverage were greater. In addition, our functional genes prediction and qPCR results demonstrated that energy/fat processing microorganisms and functional genes are enriched with increasing altitude, which likely enhanced metabolic functions and supported wild sables to survive in elevated habitats. Overall, our results improve the knowledge of the ecological impact of habitat change, providing insights into wild animal protection at the mountain area with hash climate conditions

Effects of Lactobacillus plantarum on the Fermentation Profile and Microbiological Composition of Wheat Fermented Silage Under the Freezing and Thawing Low Temperatures

The corruption and/or poor quality of silages caused by low temperature and freeze-thaw conditions makes it imperative to identify effective starters and low temperature silage fermentation technology that can assist the animal feed industry and improve livestock productivity. The effect of L. plantarum QZ227 on the wheat silage quality was evaluated under conditions at constant low temperatures followed by repeated freezing and thawing at low temperatures. QZ227 became the predominant strain in 10 days and underwent a more intensive lactic acid bacteria fermentation than CK. QZ227 accumulated more lactic acid, but lower pH and ammonia nitrogen in the fermentation. During the repeated freezing and thawing process, the accumulated lactic acid in the silage fermented by QZ227 remained relatively stable. Relative to CK, QZ227 reduced the abundance of fungal pathogens in silage at a constant 5°C, including Aspergillus, Sporidiobolaceae, Hypocreaceae, Pleosporales, Cutaneotrichosporon, Alternaria, and Cystobasidiomycetes. Under varying low temperature conditions from days 40 to days 60, QZ227 reduced the pathogenic abundance of fungi such as Pichia, Aspergillus, Agaricales, and Plectosphaerella. QZ227 also reduced the pathogenic abundance of Mucoromycota after the silage had been exposed to oxygen. In conclusion, QZ227 can be used as a silage additive in the fermentation process at both constant and variable low temperatures to ensure fast and vigorous fermentation because it promotes the rapid accumulation of lactic acid, and reduces pH values and aerobic corruption compared to the CK

Failure Analysis of a New Irrigation Water Allocation Mode Based on Copula Approaches in the Zhanghe Irrigation District, China

The risk analysis of an irrigation water allocation strategy based on physical mechanisms is critically important in practice. Conventional risk analysis only considers the role of the channel system and ignores the factors related to on-farm ponds. This paper proposes a channel-pond joint water supply mode (CPJM) based on copula approaches. Two copulas, the Plackett copula and No.16 copula, are chosen and two types of analyses are carried out with the proposed mode: (1) a risk assessment of CPJM with joint probability and conditional probability; and (2) determination of the water supply strategy given the pond water supply frequency. With a case study of the second channel in the Zhanghe Irrigation District (ZID), Southern China, nine combinations of channel water supply frequency (CWSF) and pond water supply frequency (PWSF) are studied. The results reveal that the failure probabilities of the joint distribution and the conditional distribution of the CPJM are 0.02%–16.54% and 0.45%–33.08%, respectively, with corresponding return period of 42–5000 and 10–222 years. Nevertheless, a previous study has shown that the real probability is 33.3%, which means that the return period is equals to three years. Therefore, the objective failure evaluation of the irrigation water-use strategy is useful for water saving in this channel system. Moreover, the irrigation water allocation strategy can be determined and the failure charts relating the CWSF and PWSF can be obtained for a predetermined PWSF. Thus, the channel-pond joint water supply mode provides a more reasonable estimate of the irrigation water allocation strategy reliability

Immune-mediated disease associated microbial community responded to PAH stress in phyllosphere of roadside greenspaces in Shanghai

Microorganisms in urban greenspaces play key roles in ecosystem service provision and potentially influence human health. Increasing evidence suggests that anthropogenic disturbance poses constant stress on urban microbial communities, yet, as previous studies have focused on non-contaminated greenspaces, it has remained largely unknown how microorganisms respond to anthropogenic stress in roadside greenspaces with contamination. Our previous effort determined phyllosphere PAHs of camphor trees in 84 sites of roadside greenspaces along the urban-rural gradient in Shanghai. Here, we further investigated the phyllosphere microbial communities (PMCs) of the same sites across the same urban categories, including urban, suburban, and rural areas using high-throughput DNA sequencing. We aimed to explore how PMCs, especially those associated with immune-mediated diseases (IMDs), were affected by PAHs and the surrounding land-use types. We found that several microorganisms associated with increasing IMD risk were stimulated by PAHs. The composition of PMCs differed between the three urban categories which can be largely explained by the variation of phyllosphere PAH concentration and the surrounding land-use types. Similar to our previous study, suburban areas were linked with the most potential adverse health effects, where we observed the lowest bacterial diversity, the highest relative abundance of IMD-associated bacteria, and the highest relative abundance of Pathotroph. Urban greenblue infrastructure (GBI) was positively correlated with the diversity of PMCs, whereas urban grey infrastructure tended to homogenize PMCs. Notably, GBI also reduced the relative abundance of IMD-associated and pathogenic microbes, indicating the potential health benefits of GBI in land-use planning. Taken together, our study emphasizes the need to further investigate environmental communities in contaminated traffic environments, as human microbiomes are directly exposed to risky microorganisms.Peer reviewe

Neighborhood garden's age shapes phyllosphere microbiota associated with respiratory diseases in cold seasons

Neighborhood gardens serve as sensitive sites for human microbial encounters, with phyllosphere microbes directly impacting our respiratory health. Yet, our understanding remains limited on how factors like season, garden age, and land use shape the risk of respiratory diseases (RDs) tied to these garden microbes. Here we examined the microbial communities within the phyllosphere of 72 neighborhood gardens across Shanghai, spanning different seasons (warm and cold), garden ages (old and young), and locales (urban and rural). We found a reduced microbial diversity during the cold season, except for Gammaproteobacteria which exhibited an inverse trend. While land use influenced the microbial composition, urban and rural gardens had strikingly similar microbial profiles. Alarmingly, young gardens in the cold season hosted a substantial proportion of RDs-associated species, pointing towards increased respiratory inflammation risks. In essence, while newer gardens during colder periods show a decline in microbial diversity, they have an increased presence of RDs-associated microbes, potentially escalating respiratory disease prevalence. This underscores the pivotal role the garden age plays in enhancing both urban microbial diversity and respiratory health

Neighborhood garden's age shapes phyllosphere microbiota associated with respiratory diseases in cold seasons

Neighborhood gardens serve as sensitive sites for human microbial encounters, with phyllosphere mi-crobes directly impacting our respiratory health. Yet, our understanding remains limited on how factors like season, garden age, and land use shape the risk of respiratory diseases (RDs) tied to these garden microbes. Here we examined the microbial communities within the phyllosphere of 72 neighborhood gardens across Shanghai, spanning different seasons (warm and cold), garden ages (old and young), and locales (urban and rural). We found a reduced microbial diversity during the cold season, except for Gammaproteobacteria which exhibited an inverse trend. While land use influenced the microbial composition, urban and rural gardens had strikingly similar microbial profiles. Alarmingly, young gar-dens in the cold season hosted a substantial proportion of RDs-associated species, pointing towards increased respiratory inflammation risks. In essence, while newer gardens during colder periods show a decline in microbial diversity, they have an increased presence of RDs-associated microbes, potentially escalating respiratory disease prevalence. This underscores the pivotal role the garden age plays in enhancing both urban microbial diversity and respiratory health.(c) 2023 The Authors. Published by Elsevier B.V. on behalf of Chinese Society for Environmental Sciences, Harbin Institute of Technology, Chinese Research Academy of Environmental Sciences. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Peer reviewe

Urbanization influences the indoor transfer of airborne antibiotic resistance genes, which has a seasonally dependent pattern

Over the last few years, the cumulative use of antibiotics in healthcare institutions, as well as the rearing of livestock and poultry, has resulted in the accumulation of antibiotic resistance genes (ARGs). This presents a substantial danger to human health worldwide. The characteristics of airborne ARGs, especially those transferred from outdoors to indoors, remains largely unexplored in neighborhoods, even though a majority of human population spends most of their time there. We investigated airborne ARGs and mobile genetic element (MGE, IntI1), plant communities, and airborne microbiota transferred indoors, as well as respiratory disease (RD) prevalence using a combination of metabarcode sequencing, real-time quantitative PCR and questionnaires in 72 neighborhoods in Shanghai. We hypothesized that (i) urbanization regulates ARGs abundance, (ii) the urbanization effect on ARGs varies seasonally, and (iii) land use types are associated with ARGs abundance. Supporting these hypotheses, during the warm season, the abundance of ARGs in peri-urban areas was higher than in urban areas. The abundance of ARGs was also affected by the surrounding land use and plant communities: an increase in the proportion of gray infrastructure (e.g., residential area) around neighborhoods can lead to an increase in some ARGs (mecA, qnrA, ermB and mexD). Additionally, there were variations observed in the relationship between ARGs and bacterial genera in different seasons. Specifically, Stenotrophomonas and Campylobacter were positively correlated with vanA during warm seasons, whereas Pseudomonas, Bacteroides, Treponema and Stenotrophomonas positively correlated with tetX in the cold season. Interstingly, a noteworthy positive correlation was observed between the abundance of vanA and the occurrence of both rhinitis and rhinoconjunctivitis. Taken together, our study underlines the importance of urbanization and season in controlling the indoor transfer of airborne ARGs. Furthermore, we also highlight the augmentation of green–blue infrastructure in urban environments has the potential to mitigate an excess of ARGs

Screening of Cold-Adapted Strains and Its Effects on Physicochemical Properties and Microbiota Structure of Mushroom Residue Composting

Composting is an effective way to dispose of agricultural waste; however, its application is limited in the winter and in areas with low average annual temperatures. This study screened out a composite microbial agent (CMA) including Bacillus (B.) cereus QS7 and B. pumilus QM6 that could grow at 10–15 °C and investigated the effects of the CMA as an inoculant on the physicochemical parameters and microbial communities of compost made from mushroom residue mixed with sheep manure. The results showed that CMA inoculation prolonged the days of high temperature above 60 °C. When the ratio of mushroom residue to sheep manure was at 9:1, compost increased the accumulation of nitrogen, and reduced the electrical conductivity (EC). Under this ratio, the inoculation of CMA accelerated the degradation of organic matter (OM) (decreased by 12.22%) and total organic carbon (TOC) (decreased by 8.13%) and increased the germination rate and seed germination index (GI) to 100% and 106.86, respectively. Microbial community structure analysis showed that the relative abundance of Flavobacterium was 30.62% on day 15 and was higher after CMA inoculation in the high mushroom residue condition, which was higher than that of other groups, and the relative abundance of thermophilic fungi increased. This study demonstrated that CMA improves the quality and efficiency of mushroom residue and sheep manure composting, and it provides evidence to improve the efficiency of low-temperature composting

Tailoring Defect Passivation for Efficient and Stable Perovskite Solar Cells via an Ionic Liquid Additive

As a shining star in the photovoltaic community, perovskite solar cells (PSCs) have been making significant progress in recent years. However, poor long-term operation stability caused by various defects seriously restricts their commercialization process. In this work, a multifunctional ionic liquid passivator, 1-aminoethyl-3-methylimidazolium tetrafluoroborate (AMFB), is incorporated to passivate A+, B2+, and X– defects in the perovskite absorber and enhance device stability further. It is found that AMFB can cooperate with undercoordinated Pb2+ and from hydrogen bonds with organic cations and I– ions, leading to a sufficiently passivated perovskite film. Besides, the more suitable energy arrangement and hydrophobicity of the resultant interface contribute to more facilitated carrier transport and durable stability of devices. As a result, the champion power conversion efficiency (PCE) of optimized devices significantly increases from 22.16 to 24.41% with negligible hysteresis, and the large-area devices also boost from 19.86 to 23.24%. Surprisingly, the unpackaged AMFB devices demonstrate excellent robustness against moisture and thermal stresses, maintaining over 90% of their initial PCE after 1500 h of continuous heating at 85 °C and 500 h of aging in moisture air with relative humidity of 70 ± 5%. This work provides an effective and feasible method for improving the performance of PSCs and facilitating their commercialization process