Plasmonic gold nanodiscs using piezoelectric substrate birefringence for liquid sensing

This article presents the simulation, fabrication, and experimental characterization of a surface plasmonic resonance (SPR) sensor integrated with an acoustic sensing compatible substrate. The SPR sensor is designed to work in the visible region with gold nanodisc arrays fabricated on LiNbO3, which is both piezoelectric and birefringent. A linear relationship between resonance wavelength and varying liquid refractive indices were observed in experiments, and a sensitivity of 165 nm/refractive index unit was obtained. Polarization effects of the birefringent property of the Y-cut LiNbO3 substrate have been investigated, which can also be applied to X-cut LiNbO3. Our study demonstrates the feasibility of an SPR sensor device utilizing a birefringent substrate, which has acoustic wave compatibility and can pave the way toward much more robust and flexible biosensing device

Hybridisation of plasmonic and acoustic biosensing devices

Monolithically integrating multiple sensing technologies shows a great potential to perform quantitative measurements for multiple biomarkers of diseases and also provide more insight towards one single biochemical event. The localised surface plasmon resonance spectroscopy measures the change in the refractive index arising from the molecular adsorption on the metallic nanostructures. Acoustic sensors, such as surface acoustic wave sensor and quartz crystal microbalance, measure the variation of its mechanical oscillation caused by the sum of the deposited molecules and the solvent coupled to the adsorbed molecules. Both techniques are known independently as having applications in in-situ, label-free sensing and analysis of biological binding reactions. Due to their complementary properties, the integration of both can prove to be a valuable tool for studying biomolecules on sensing surface. This thesis reports on the development of a hybrid biosensing device that integrates localised surface plasmonic sensing and acoustic sensing technologies. Gold nanodisk arrays as localised surface plasmon resonance sensing device was studied in visible region using three substrates: borosilicate glass, lithium niobate and quartz. The design, simulation, fabrication and characterisation of the gold nanodisk arrays, and the sensing performance optimisation were investigated using glass substrate. Lithium niobate, as a piezoelectric material has surface acoustic wave compatibility and this study can pave the way towards the development of hybrid sensing devices. The study on lithium niobate demonstrated the feasibility of a localised surface plasmon resonance device utilising a high refractive index, birefringent and piezoelectric substrate. Using quartz as the substrate, the design and fabrication of a hybrid sensor were performed, which integrated localised surface plasmonic resonance into a quartz crystal microbalance for studying biochemical surface binding reactions. The coupling of localised plasmon resonance nanostructures and a quartz crystal microbalance allows optical spectra and quartz crystal microbalance resonant frequency shifts to be recorded simultaneously, and analysed in real time for a given surface adsorption process. This integration has the potential to be miniaturised for application in point-of-care diagnostics

Hybrid localized surface plasmon resonance and quartz crystal microbalance sensor for label free biosensing

We report on the design and fabrication of a hybrid sensor that integrates transmission-mode localized surface plasmonic resonance (LSPR) into a quartz crystal microbalance (QCM) for studying biochemical surface reactions. The coupling of LSPR nanostructures and a QCM allows optical spectra and QCM resonant frequency shifts to be recorded simultaneously and analyzed in real time for a given surface adsorption process. This integration simplifies the conventional combination of SPR and QCM and has the potential to be miniaturized for application in point-of-care (POC) diagnostics. The influence of antibody-antigen recognition effect on both the QCM and LSPR has been analyzed and discussed.`

Monolithic Integration of a Plasmonic Sensor with CMOS Technology

Monolithic integration of nanophotonic sensors with CMOS detectors can transform the laboratory based nanophotonic sensors into practical devices with a range of applications in everyday life. In this work, by monolithically integrating an array of gold nanodiscs with the CMOS photodiode we have developed a compact and miniaturized nanophotonic sensor system having direct electrical read out. Doing so eliminates the need of expensive and bulky laboratory based optical spectrum analyzers used currently for measurements of nanophotonic sensor chips. The experimental optical sensitivity of the gold nanodiscs is measured to be 275 nm/RIU which translates to an electrical sensitivity of 5.4 V/RIU. This integration of nanophotonic sensors with the CMOS electronics has the potential to revolutionize personalized medical diagnostics similar to the way in which the CMOS technology has revolutionized the electronics industry

Ultra-narrow line width polarization-insensitive filter using a symmetry-breaking selective plasmonic metasurface

Plasmonic metasurfaces provide unprecedented control of the properties of light. By designing symmetry-breaking nanoholes in a metal sheet and engineering the optical properties of the metal using geometry, highly selective transmission and polarisation control of light is obtained. To date such plasmonic filters have exhibited broad (> 200 nm) transmission linewidths in the NIR and as such are unsuitable for applications requiring narrow passbands, e.g. multi-spectral imaging. Here we present a novel sub-wavelength elliptical and circular nanohole array in a metallic film that simultaneously exhibits high transmission efficiency, polarisation insensitivity and narrow linewidth. The experimentally obtained linewidth is 79 nm with a transmission efficiency of 44%. By examining the electric and magnetic field distributions for various incident polarisations at the transmission peak we show that the narrowband characteristics are due to a Fano resonance. Good agreement is obtained between the experimental data, simulations and analytical calculations. Our design can be modified to operate in other regions of the electromagnetic spectrum and these filters may be integrated with suitable detectors such as photodiodes and single photon avalanche diode (SPAD) arrays

Calibration of the Radical Installation Limit Error of the Accelerometer in the Gravity Gradient Instrument

Gravity gradient instrument (GGI) is the core of the gravity gradiometer, so the structural error of the sensor has a great impact on the measurement results. In order not to affect the aimed measurement accuracy, limit error is required in the installation of the accelerometer. In this paper, based on the established measuring principle model, the radial installation limit error is calibrated, which is taken as an example to provide a method to calculate the other limit error of the installation under the premise of ensuring the accuracy of the measurement result. This method provides the idea for deriving the limit error of the geometry structure of the sensor, laying the foundation for the mechanical precision design and physical design

Characterization of extracellular cellulose-degrading enzymes from Bacillus thuringiensis strains

The gram-positive spore-forming bacteria, Bacillus thuringiensis (Bt) strains produced novel cellulases which could liberate glucose from soluble cellulose, carboxymethyl cellulose (CMC), and insoluble crystalline cellulose. The maximal cellulase activities were obtained after 60 hrs incubation at 28\ubaC in a LB broth medium with 1% CMC. Maximum CMCase activities were got at 40\ubaC and pH 4.0, respectively, and more than 50% of its maximal activity was retained at 40-60\ubaC for 1 hr, while approximately 40% of its maximal activity was also retained after incubating at 70\ubaC for 1 hr. Most metal ions and reagents such as Ca2+, Mg2+, Cd2+, Pb2+, Zn2+, Cu2+, EDTA, and SDS inhibited the enzyme activities, but K+ and Mn2+ activated the activities. The enzymes from Bacillus thuringiensis strains could be applied in bioconversion of lignocellulosic biomass into fermentable sugars

Mathematical Modeling of the Working Principle of Gravity Gradient Instrument

Gravity field is of great significance in geoscience, national economy and national security, and gravitational gradient measurement has been extensively studied due to its higher accuracy than gravity measurement. Gravity gradient sensor, being one of core devices of the gravity gradient instrument, plays a key role in measuring accuracy. Therefore, this paper starts from analyzing the working principle of the gravity gradient sensor by Newton's law, and then considers the relative motion between inertial and non-inertial systems to build a relatively adequate mathematical model, laying a foundation for the measurement error calibration, measurement accuracy improvement

Analysis of the Risk Factors for Elevated D-Dimer Level After Breast Cancer Surgery: A Multicenter Study Based on Nursing Follow-Up Data

D-dimer level is often used to assess the severity of trauma as well as the risk of thrombosis. This study investigated the risk factors for high postoperative D-dimer level. This study included a total of 2706 patients undergoing breast cancer surgery to examine the associations between various clinicopathological factors and variation in D-dimer levels. After adjusting for other factors, T stage, neoadjuvant chemotherapy, blood loss, surgery type, diabetes, and elevated leukocyte and neutrophil counts were found to be significant risk factors for D-dimer variation. This study identified several factors associated with elevated D-dimer levels and consequent thrombosis after breast cancer surgery, which may aid in the development of more precise preventive measures and interventions as well as serve as a reference for future research